LOCUS       sequence-358624-        8709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding a protein fusion composed of an
            N-terminal ER targeting sequence of hemagglutinin (HA), followed by 
            EGFP and murine CDMPR (without its signal peptide).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Buser DP, Bader G, Spiess M
  TITLE     Retrograde transport of CDMPR depends on several machineries as 
            analyzed by sulfatable nanobodies.
  JOURNAL   Life Sci Alliance. 2022 Mar 21;5(7). pii: 5/7/e202101269. doi: 
            10.26508/lsa.202101269. Print 2022 Mar.
  PUBMED    35314489
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-03-21"; volume: "5(7). pii: 5/7/e202101269. 
            doi"; pages: " 10.26508/lsa.202101269. Print 2022 Mar"
COMMENT     Sequence Label: pQCXIP-EGFP-CDMPR
FEATURES             Location/Qualifiers
     source          1..8709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2315..2317,2318..2320,2321..3031)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2315..2317,2318..2320,2321..3031)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2360..2381)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2621..2640)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2968..2989
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3836..4410,4411..4413,4414..4422)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4003..4020)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4230..4249
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4442..5041
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4442..4461)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4938..4958
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5415..5840
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5943..5965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6101..6121)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6106..6435
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6286..6421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6457..6476
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6557..6574)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6728..7316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6808..6827)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7487..8278,8279..8347))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8110..8129
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8348..8452)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8520..8538
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(8638..8709,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgcc cgccaccatg aagacgatca tcgccctgag
     2281 ctacatcttc tgcctggtgt tcgccaggga atctatggtg agcaagggcg aggagctgtt
     2341 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     2401 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     2461 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     2521 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2581 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2641 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2701 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2761 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2821 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2881 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     2941 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     3001 gatcactctc ggcatggacg agctgtacaa gggtaccaca gaagaaaaat cgtgtgacct
     3061 ggtaggagag aaggataagg agtcaaagaa cgaggtggct ctcctggaga ggttacggcc
     3121 actgtttaac aaaagttttg agagtactgt gggccagggc tcagacacat acagctacat
     3181 attcagagta tgccgggaag ctagcaacca ctcctctgga gcaggcctgg tccagatcaa
     3241 caaaagcaat gacaaggaga cagtggttgg gagaatcaac gagactcaca tcttcaatgg
     3301 aagtaattgg atcatgctga tatataaagg gggtgatgaa tatgacaacc actgtggcaa
     3361 agagcagcgc cgtgcagtgg tgatgatctc ctgcaatcgg cacacactag cggctaattt
     3421 taacccagtg tctgaggaac gaggcaaagt ccaggattgc ttctacctct ttgagatgga
     3481 tagcagcctg gcctgttcac cagaggtctc acacctcagt gtgggctcga tcttacttgt
     3541 catatttgca tcattggttg ctgtctatat cattgggggt ttcttatacc agcgattggt
     3601 agtgggggcc aagggaatgg agcagtttcc tcatctggcc ttctggcagg atcttggcaa
     3661 cctagtagct gatggttgtg actttgtgtg ccgatccaaa ccccgcaatg tgcctgcagc
     3721 atatcgtgga gtgggagatg accagctggg ggaagagtcg gaagaaaggg atgatcatct
     3781 gctaccaatg tgataattaa ttaacggatc cggaattccg cccccccccc ccccccccct
     3841 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     3901 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     3961 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4021 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4081 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4141 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4201 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     4261 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     4321 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     4381 tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga gacgaccttc
     4441 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     4501 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     4561 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4621 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4681 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4741 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4801 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4861 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4921 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4981 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5041 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atggctccga
     5101 ccgaagccac ccggggcggc cccgccgacc ccgcacccgc ccccgaggcc caccgactct
     5161 agtcgagggc tgcagcgctg cagaggccga gtgcagaact gctccaaagg gacctcaagg
     5221 ctttccgagg gacactaggc tgactccatc gagccagtgt agagataagc ttatcgatta
     5281 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat
     5341 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc
     5401 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     5461 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     5521 ggaacagatg gaacagggtc gaccctagag aaccatcaga tgtttccagg gtgccccaag
     5581 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     5641 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     5701 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     5761 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     5821 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     5881 caccgaccca ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa
     5941 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6001 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     6061 acccagtcac gtagcgatag cggagtgtag atccggctgt ggaatgtgtg tcagttaggg
     6121 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6181 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6241 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6301 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6361 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6421 ctaggctttt gcaaaaagct tactggctta actatgcggc atcagagcag attgtactga
     6481 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6541 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6601 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6721 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6781 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6841 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6901 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6961 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7021 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7081 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7141 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     7201 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7501 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7561 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7621 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7681 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7741 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7801 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7861 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7921 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7981 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8041 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8101 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8161 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8221 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8281 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8341 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8401 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8461 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     8521 ataggcgtat cacgaggccc tttcgtcttc aagaattagc ttggccattg catacgttgt
     8581 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
     8641 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     8701 atatggagt
//
