LOCUS       sequence-358639-       13129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible CRISPRi plasmid with gRNA targeting to psbD gene in 
            Synechocystis 6803.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Liu D, Johnson VM, Pakrasi HB
  TITLE     A Reversibly Induced CRISPRi System Targeting Photosystem II in the 
            Cyanobacterium Synechocystis sp. PCC 6803.
  JOURNAL   ACS Synth Biol. 2020 Jun 19;9(6):1441-1449. doi: 
            10.1021/acssynbio.0c00106. Epub 2020 May 21.
  PUBMED    32379958
REFERENCE   2  (bases 1 to 13129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00106"; journalName: "ACS Synth Biol"; date: 
            "2020-06-19- 19"; volume: "9"; issue: "6"; pages: "1441-1449"
COMMENT     Sequence Label: pCRISPRi-D2
FEATURES             Location/Qualifiers
     source          1..13129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      809..1203
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            1545..1632
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2871..3842
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             4353..5192
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             5179..6030
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6426..7262)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNVSVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     primer_bind     complement(7258..7274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7258..7274)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7271..7293)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7282..7298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7306..7312,7313..7330,7331..7336))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             7731..7739
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(11842..12657)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(11842..12657)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12565..12584
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12649..12668
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 aaaaaaatga ccttcataaa tcgctaatct acaacagtag aaattattta aagttcttag
       61 accggctact aatacaaaag gctctagaca tctacaacag tagaaattat ttaaagttct
      121 tagactgaat tatctgaagg cacaggaata gtagcatcta caacagtaga aattatttaa
      181 agttcttaga cgactggggc gcgtccgact gatctacaac agtagaaatt atttaaagtt
      241 cttagacccg tttttgccta aatcagcttc tacctccttt gtatattata aacttacccg
      301 attcattacg accagtctaa aaagcgcctg aattcgcgac cttctcgtta ctgacaggaa
      361 aatgggccat tggcaaccag ggaaagatga acgtgatgat gttcacaatt tgctgaattg
      421 tggccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca tgctccagcc
      481 gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat tcctttccct
      541 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
      601 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
      661 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
      721 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
      781 gtgcccgtca ctcccgtact aactgtcacg aacccctgca ataactgtca cgcccccctg
      841 caataactgt cacgaacccc tgcaataact gtcacgcccc caaacctgca aacccagcag
      901 gggcgggggc tggcggggtg ttggaaaaat ccatccatga ttatctaaga ataatccact
      961 aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct tgcccaatat gcccggccag
     1021 aggccggata gctggtctat tcgctgcgct aggctacaca ccgccccacc gctgcgcggc
     1081 agggggaaag gcgggcaaag cccgctaaac cccacaccaa accccgcaga aatacgctgg
     1141 agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg cgcgtgtgca
     1201 gccccgcagg gcctgtctcg gtcgatcatt cagcccggct catccttctg gcgtggcggc
     1261 agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg catccattgc cgccatgagc
     1321 cgatcctccg gccactcgct gctgttcacc ttggccaaaa tcatggcccc caccagcacc
     1381 ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc ttgcccgcac ccgctgaatt
     1441 tcggcattga ttcgcgctcg ttgttcttcg agcttggcca gccgatccgc cgccttgttg
     1501 ctccccttaa ccatcttgac accccattgt taatgtgctg tctcgtaggc tatcatggag
     1561 gcacagcggc ggcaatcccg accctacttt gtaggggagg gcgcacttac cggtttctct
     1621 tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg ccattgcatg
     1681 gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt acggcgaaaa
     1741 ccggcagcag gtcgggcggc caatcggcca gggccaaggc cgactacatc cagcgcgaag
     1801 gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga atccgggcac atgccggagt
     1861 tcgtcgagcg gcccgccgac tactgggatg ctgccgacct gtatgaacgc gccaatgggc
     1921 ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac cagcagaagg
     1981 cgctggcgtc cgagttcgcc cagcacctga ccggtgccga gcgcctgccg tatacgctgg
     2041 ccatccatgc cggtggcggc gagaacccgc actgccacct gatgatctcc gagcggatca
     2101 atgacggcat cgagcggccc gccgctcagt ggttcaagcg gtacaacggc aagaccccgg
     2161 agaagggcgg ggcacagaag accgaagcgc tcaagcccaa ggcatggctt gagcagaccc
     2221 gcgaggcatg ggccgaccat gccaaccggg cattagagcg ggctggccac gacgcccgca
     2281 ttgaccacag aacacttgag gcgcagggca tcgagcgcct gcccggtgtt cacctggggc
     2341 cgaacgtggt ggagatggaa ggccggggca tccgcaccga ccgggcagac gtggccctga
     2401 acatcgacac cgccaacgcc cagatcatcg acttacagga ataccgggag gcaatagacc
     2461 atgaacgcaa tcgacagagt gaagaaatcc agaggcatca acgagttagc ggagcagatc
     2521 gaaccgctgg cccagagcat ggcgacactg gccgacgaag cccggcaggt catgagccag
     2581 acccagcagg ccagcgaggc gcaggcggcg gagtggctga aagcccagcg ccagacaggg
     2641 gcggcatggg tggagctggc caaagagttg cgggaggtag ccgccgaggt gagcagcgcc
     2701 gcgcagagcg cccggagcgc gtcgcggggg tggcactgga agctatggct aaccgtgatg
     2761 ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt gctcgacctg
     2821 acgccactga caaccgagga cggctcgatc tggctgcgct tggtggcccg atgaagaacg
     2881 acaggacttt gcaggccata ggccgacagc tcaaggccat gggctgtgag cgcttcgata
     2941 tcggcgtcag ggacgccacc accggccaga tgatgaaccg ggaatggtca gccgccgaag
     3001 tgctccagaa cacgccatgg ctcaagcgga tgaatgccca gggcaatgac gtgtatatca
     3061 ggcccgccga gcaggagcgg catggtctgg tgctggtgga cgacctcagc gagtttgacc
     3121 tggatgacat gaaagccgag ggccgggagc ctgccctggt agtggaaacc agcccgaaga
     3181 actatcaggc atgggtcaag gtggccgacg ccgcaggcgg tgaacttcgg gggcagattg
     3241 cccggacgct ggccagcgag tacgacgccg acccggccag cgccgacagc cgccactatg
     3301 gccgcttggc gggcttcacc aaccgcaagg acaagcacac cacccgcgcc ggttatcagc
     3361 cgtgggtgct gctgcgtgaa tccaagggca agaccgccac cgctggcccg gcgctggtgc
     3421 agcaggctgg ccagcagatc gagcaggccc agcggcagca ggagaaggcc cgcaggctgg
     3481 ccagcctcga actgcccgag cggcagctta gccgccaccg gcgcacggcg ctggacgagt
     3541 accgcagcga gatggccggg ctggtcaagc gcttcggtga tgacctcagc aagtgcgact
     3601 ttatcgccgc gcagaagctg gccagccggg gccgcagtgc cgaggaaatc ggcaaggcca
     3661 tggccgaggc cagcccagcg ctggcagagc gcaagcccgg ccacgaagcg gattacatcg
     3721 agcgcaccgt cagcaaggtc atgggtctgc ccagcgtcca gcttgcgcgg gccgagctgg
     3781 cacgggcacc ggcaccccgc cagcgaggca tggacagggg cgggccagat ttcagcatgt
     3841 agtgcttgcg ttggtactca cgcctgttat actatgagta ctcacgcaca gaagggggtt
     3901 ttatggaata cgaaaaaagc gcttcagggt cggtctacct gatcaaaagt gacaagggct
     3961 attggttgcc cggtggcttt ggttatacgt caaacaaggc cgaggctggc cgcttttcag
     4021 tcgctgatat ggccagcctt aaccttgacg gctgcacctt gtccttgttc cgcgaagaca
     4081 agcctttcgg ccccggcaag tttctcggtg actgatatga aagaccaaaa ggacaagcag
     4141 accggcgacc tgctggccag ccctgacgct gtacgccaag cgcgatatgc cgagcgcatg
     4201 aaggccaaag ggatgcgtca gcgcaagttc tggctgaccg acgacgaata cgaggcgctg
     4261 cgcgagtgcc tggaagaact cagagcggcg cagggcgggg gtagtgaccc cgccagcgcc
     4321 taaccaccaa ctgcctgcaa aggaggcaat caatggctac ccataagcct atcaatattc
     4381 tggaggcgtt cgcagcagcg ccgccaccgc tggactacgt tttgcccaac atggtggccg
     4441 gtacggtcgg ggcgctggtg tcgcccggtg gtgccggtaa atccatgctg gccctgcaac
     4501 tggccgcaca gattgcaggc gggccggatc tgctggaggt gggcgaactg cccaccggcc
     4561 cggtgatcta cctgcccgcc gaagacccgc ccaccgccat tcatcaccgc ctgcacgccc
     4621 ttggggcgca cctcagcgcc gaggaacggc aagccgtggc tgacggcctg ctgatccagc
     4681 cgctgatcgg cagcctgccc aacatcatgg ccccggagtg gttcgacggc ctcaagcgcg
     4741 ccgccgaggg ccgccgcctg atggtgctgg acacgctgcg ccggttccac atcgaggaag
     4801 aaaacgccag cggccccatg gcccaggtca tcggtcgcat ggaggccatc gccgccgata
     4861 ccgggtgctc tatcgtgttc ctgcaccatg ccagcaaggg cgcggccatg atgggcgcag
     4921 gcgaccagca gcaggccagc cggggcagct cggtactggt cgataacatc cgctggcagt
     4981 cctacctgtc gagcatgacc agcgccgagg ccgaggaatg gggtgtggac gacgaccagc
     5041 gccggttctt cgtccgcttc ggtgtgagca aggccaacta tggcgcaccg ttcgctgatc
     5101 ggtggttcag gcggcatgac ggcggggtgc tcaagcccgc cgtgctggag aggcagcgca
     5161 agagcaaggg ggtgccccgt ggtgaagcct aagaacaagc acagcctcag ccacgtccgg
     5221 cacgacccgg cgcactgtct ggcccccggc ctgttccgtg ccctcaagcg gggcgagcgc
     5281 aagcgcagca agctggacgt gacgtatgac tacggcgacg gcaagcggat cgagttcagc
     5341 ggcccggagc cgctgggcgc tgatgatctg cgcatcctgc aagggctggt ggccatggct
     5401 gggcctaatg gcctagtgct tggcccggaa cccaagaccg aaggcggacg gcagctccgg
     5461 ctgttcctgg aacccaagtg ggaggccgtc accgctgatg ccatggtggt caaaggtagc
     5521 tatcgggcgc tggcaaagga aatcggggca gaggtcgata gtggtggggc gctcaagcac
     5581 atacaggact gcatcgagcg cctttggaag gtatccatca tcgcccagaa tggccgcaag
     5641 cggcaggggt ttcggctgct gtcggagtac gccagcgacg aggcggacgg gcgcctgtac
     5701 gtggccctga accccttgat cgcgcaggcc gtcatgggtg gcggccagca tgtgcgcatc
     5761 agcatggacg aggtgcgggc gctggacagc gaaaccgccc gcctgctgca ccagcggctg
     5821 tgtggctgga tcgaccccgg caaaaccggc aaggcttcca tagatacctt gtgcggctat
     5881 gtctggccgt cagaggccag tggttcgacc atgcgcaagc gccgccagcg ggtgcgcgag
     5941 gcgttgccgg agctggtcgc gctgggctgg acggtaaccg agttcgcggc gggcaagtac
     6001 gacatcaccc ggcccaaggc ggcaggctga ccccccccac tctattgtaa acaagacatt
     6061 tttatctttt atattcaatg gcttattttc ctgctaattg gtaataccat gaaaaatacc
     6121 atgctcagaa aaggcttaac aatattttga aaaattgcct actgagcgct gccgcacagc
     6181 tccataggcc gctttcctgg ctttgcttcc agatgtatgc tcttcttaat tgacaattga
     6241 caattcccca cttagataaa aaatccggtc aggatgcggt caacattata caaaaactgt
     6301 ccaaagaaca gggccgtatt gtgttgctga ttacctatag tcactgtgaa aaagcaacaa
     6361 aaaacccgcg ggagcgggct aacattgaat tgaagtcagt tttttagtaa ttgccaaaac
     6421 tgtaattatt gcagaaagcc atcccgtccc tgacgaatat cacgcggtga ccagttaaac
     6481 tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg ataggcgatg
     6541 tcagtaacgc tggcctcact gtggcgtagc agatgtcggg ctttcatcag tcgcaggcgg
     6601 ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg tagcgtacgc
     6661 agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc aaaatggtcc
     6721 tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa
     6781 ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact ggcggtcgag
     6841 ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc
     6901 aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc ttgtggcagc
     6961 aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag cacattggtc
     7021 agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac gaaacagacc
     7081 gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt gccatgttcg
     7141 acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg cgggagccgg
     7201 ggttctatcg ccacggacac gttaccagac ggaaaaaaat ccacactatg taatacggtc
     7261 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7321 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taattgagac ttttctgatt
     7381 ttgcaaaggt tttgctttag ttaaacccaa ttgattagtg tcccctgccc atttggtggg
     7441 ggattattat ttttaagata atcctatttt ttggagtgag gccagttacc tattagacgc
     7501 gcgactcgaa agtcgttcag gggagttgga acggcttcca aaaacctttc cccgctggtg
     7561 ttccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
     7621 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa
     7681 tgaataccgg tgataccagc atcgtcttga tgcccttggc agcaccctgc taaggaggta
     7741 acaacaagat gtcaatttat caagaatttg ttaataaata tagtttaagt aaaactctaa
     7801 gatttgagtt aatcccacag ggtaaaacac ttgaaaacat aaaagcaaga ggtttgattt
     7861 tagatgatga gaaaagagct aaagactaca aaaaggctaa acaaataatt gataaatatc
     7921 atcagttttt tatagaggag atattaagtt cggtttgtat tagcgaagat ttattacaaa
     7981 actattctga tgtttatttt aaacttaaaa agagtgatga tgataatcta caaaaagatt
     8041 ttaaaagtgc aaaagatacg ataaagaaac aaatatctga atatataaag gactcagaga
     8101 aatttaagaa tttgtttaat caaaacctta tcgatgctaa aaaagggcaa gagtcagatt
     8161 taattctatg gctaaagcaa tctaaggata atggtataga actatttaaa gccaatagtg
     8221 atatcacaga tatagatgag gcgttagaaa taatcaaatc ttttaaaggt tggacaactt
     8281 attttaaggg ttttcatgaa aatagaaaaa atgtttatag tagcaatgat attcctacat
     8341 ctattattta taggatagta gatgataatt tgcctaaatt tctagaaaat aaagctaagt
     8401 atgagagttt aaaagacaaa gctccagaag ctataaacta tgaacaaatt aaaaaagatt
     8461 tggcagaaga gctaaccttt gatattgact acaaaacatc tgaagttaat caaagagttt
     8521 tttcacttga tgaagttttt gagatagcaa actttaataa ttatctaaat caaagtggta
     8581 ttactaaatt taatactatt attggtggta aatttgtaaa tggtgaaaat acaaagagaa
     8641 aaggtataaa tgaatatata aatctatact cacagcaaat aaatgataaa acactcaaaa
     8701 aatataaaat gagtgtttta tttaagcaaa ttttaagtga tacagaatct aaatcttttg
     8761 taattgataa gttagaagat gatagtgatg tagttacaac gatgcaaagt ttttatgagc
     8821 aaatagcagc ttttaaaaca gtagaagaaa aatctattaa agaaacacta tctttattat
     8881 ttgatgattt aaaagctcaa aaacttgatt tgagtaaaat ttattttaaa aatgataaat
     8941 ctcttactga tctatcacaa caagtttttg atgattatag tgttattggt acagcggtac
     9001 tagaatatat aactcaacaa atagcaccta aaaatcttga taaccctagt aagaaagagc
     9061 aagaattaat agccaaaaaa actgaaaaag caaaatactt atctctagaa actataaagc
     9121 ttgccttaga agaatttaat aagcatagag atatagataa acagtgtagg tttgaagaaa
     9181 tacttgcaaa ctttgcggct attccgatga tatttgatga aatagctcaa aacaaagaca
     9241 atttggcaca gatatctatc aaatatcaaa atcaaggtaa aaaagaccta cttcaagcta
     9301 gtgcggaaga tgatgttaaa gctatcaagg atcttttaga tcaaactaat aatctcttac
     9361 ataaactaaa aatatttcat attagtcagt cagaagataa ggcaaatatt ttagacaagg
     9421 atgagcattt ttatctagta tttgaggagt gctactttga gctagcgaat atagtgcctc
     9481 tttataacaa aattagaaac tatataactc aaaagccata tagtgatgag aaatttaagc
     9541 tcaattttga gaactcgact ttggctaatg gttgggataa aaataaagag cctgacaata
     9601 cggcaatttt atttatcaaa gatgataaat attatctggg tgtgatgaat aagaaaaata
     9661 acaaaatatt tgatgataaa gctatcaaag aaaataaagg cgagggttat aaaaaaattg
     9721 tttataaact tttacctggc gcaaataaaa tgttacctaa ggttttcttt tctgctaaat
     9781 ctataaaatt ttataatcct agtgaagata tacttagaat aagaaatcat tccacacata
     9841 caaaaaatgg tagtcctcaa aaaggatatg aaaaatttga gtttaatatt gaagattgcc
     9901 gaaaatttat agatttttat aaacagtcta taagtaagca tccggagtgg aaagattttg
     9961 gatttagatt ttctgatact caaagatata attctataga tgaattttat agagaagttg
    10021 aaaatcaagg ctacaaacta acttttgaaa atatatcaga gagctatatt gatagcgtag
    10081 ttaatcaggg taaattgtac ctattccaaa tctataataa agatttttca gcttatagca
    10141 aagggcgacc aaatctacat actttatatt ggaaagcgct gtttgatgag agaaatcttc
    10201 aagatgtggt ttataagcta aatggtgagg cagagctttt ttatcgtaaa caatcaatac
    10261 ctaaaaaaat cactcaccca gctaaagagg caatagctaa taaaaacaaa gataatccta
    10321 aaaaagagag tgtttttgaa tatgatttaa tcaaagataa acgctttact gaagataagt
    10381 ttttctttca ctgtcctatt acaatcaatt ttaaatctag tggagctaat aagtttaatg
    10441 atgaaatcaa tttattgcta aaagaaaaag caaatgatgt tcatatatta agtatagcta
    10501 gaggtgaaag acatttagct tactatactt tggtagatgg taaaggcaat atcatcaaac
    10561 aagatacttt caacatcatt ggtaatgata gaatgaaaac aaactaccat gataagcttg
    10621 ctgcaataga gaaagatagg gattcagcta ggaaagactg gaaaaagata aataacatca
    10681 aagagatgaa agagggctat ctatctcagg tagttcatga aatagctaag ctagttatag
    10741 agtataatgc tattgtggtt tttgaggatt taaattttgg atttaaaaga gggcgtttca
    10801 aggtagagaa gcaggtctat caaaagttag aaaaaatgct aattgagaaa ctaaactatc
    10861 tagttttcaa agataatgag tttgataaaa ctgggggagt gcttagagct tatcagctaa
    10921 cagcaccttt tgagactttt aaaaagatgg gtaaacaaac aggtattatc tactatgtac
    10981 cagctggttt tacttcaaaa atttgtcctg taactggttt tgtaaatcag ttatatccta
    11041 agtatgaaag tgtcagcaaa tctcaagagt tctttagtaa gtttgacaag atttgttata
    11101 accttgataa gggctatttt gagtttagtt ttgattataa aaactttggt gacaaggctg
    11161 ccaaaggcaa gtggactata gctagctttg ggagtagatt gattaacttt agaaattcag
    11221 ataaaaatca taattgggat actcgagaag tttatccaac taaagagttg gagaaattgc
    11281 taaaagatta ttctatcgaa tatgggcatg gcgaatgtat caaagcagct atttgcggtg
    11341 agagcgacaa aaagtttttt gctaagctaa ctagtgtcct aaatactatc ttacaaatgc
    11401 gtaactcaaa aacaggtact gagttagatt atctaatttc accagtagca gatgtaaatg
    11461 gcaatttctt tgattcgcga caggcgccaa aaaatatgcc tcaagatgct gatgccaatg
    11521 gtgcttatca tattgggcta aaaggtctga tgctactagg taggatcaaa aataatcaag
    11581 agggcaaaaa actcaatttg gttatcaaaa atgaagagta ttttgagttc gtgcagaata
    11641 ggaataacta aggaattcgg gcagtgaaaa aaaaaacccc gcccctgaca gggcggggtt
    11701 ttttttaacg tggctttgcg cattaaagct agacaaaaaa atgacctttc gactctagag
    11761 aggatctgta acaacaaagc cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta
    11821 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
    11881 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
    11941 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
    12001 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
    12061 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt
    12121 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
    12181 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
    12241 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
    12301 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
    12361 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
    12421 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
    12481 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
    12541 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
    12601 catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcataac
    12661 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
    12721 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg
    12781 aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt
    12841 ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg
    12901 tggctccctc actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac
    12961 accttcttca cgaggcagac ctcagcgcta ttctgacctt gccatcacga ctgtgctggt
    13021 cattaaacgc gtattcaggc tgaccctgcg cgctgcgcag ggctttattg attccatttt
    13081 tacactgatg aatgttccgt tgcgctgccc ggattacagc tgaaagcga
//
