LOCUS       sequence-358642-        9591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EYFP driven by inducible promoter Prha-rsw.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Liu D, Johnson VM, Pakrasi HB
  TITLE     A Reversibly Induced CRISPRi System Targeting Photosystem II in the 
            Cyanobacterium Synechocystis sp. PCC 6803.
  JOURNAL   ACS Synth Biol. 2020 Jun 19;9(6):1441-1449. doi: 
            10.1021/acssynbio.0c00106. Epub 2020 May 21.
  PUBMED    32379958
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00106"; journalName: "ACS Synth Biol"; date: 
            "2020-06-19- 19"; volume: "9"; issue: "6"; pages: "1441-1449"
COMMENT     Sequence Label: pRha-rsw-EYFP
FEATURES             Location/Qualifiers
     source          1..9591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(200..1036)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNVSVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     primer_bind     complement(1032..1048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1032..1048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1045..1067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1056..1072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1080..1086,1087..1104,1105..1110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             1505..1513
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             join(1523..1525,1526..1528,1529..2242)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1829..1848)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2176..2197
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      2262..2333
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2349..2376
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    2383..2403
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(2514..3329)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2514..3329)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3237..3256
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3321..3340
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4187..4581
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            4923..5010
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6249..7220
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             7731..8570
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             8557..9408
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
ORIGIN
        1 taattgacaa ttgacaattc cccacttaga taaaaaatcc ggtcaggatg cggtcaacat
       61 tatacaaaaa ctgtccaaag aacagggccg tattgtgttg ctgattacct atagtcactg
      121 tgaaaaagca acaaaaaacc cgcgggagcg ggctaacatt gaattgaagt cagtttttta
      181 gtaattgcca aaactgtaat tattgcagaa agccatcccg tccctgacga atatcacgcg
      241 gtgaccagtt aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac
      301 agcgataggc gatgtcagta acgctggcct cactgtggcg tagcagatgt cgggctttca
      361 tcagtcgcag gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc
      421 gatgtagcgt acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat
      481 cggcaaaatg gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca
      541 ggttctcctg caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc
      601 gactggcggt cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct
      661 gtcgcacctg ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca
      721 gctcttgtgg cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat
      781 acagcacatt ggtcagacac agattatcgg tatgttcata cagatgccga tcatgatcgc
      841 gtacgaaaca gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac
      901 ccgtgccatg ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg
      961 cctgcgggag ccggggttct atcgccacgg acacgttacc agacggaaaa aaatccacac
     1021 tatgtaatac ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     1081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaattg
     1141 agacttttct gattttgcaa aggttttgct ttagttaaac ccaattgatt agtgtcccct
     1201 gcccatttgg tgggggatta ttatttttaa gataatccta ttttttggag tgaggccagt
     1261 tacctattag acgcgcgact cgaaagtcgt tcaggggagt tggaacggct tccaaaaacc
     1321 tttccccgct ggtgttccac aattcagcaa attgtgaaca tcatcacgtt catctttccc
     1381 tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt
     1441 tagactggtc gtaatgaata ccggtgatac cagcatcgtc ttgatgccct tggcagcacc
     1501 ctgctaagga ggtaacaaca agatggtgag caagggcgag gagctgttca ccggggtggt
     1561 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1621 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1681 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc
     1741 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta
     1801 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1861 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1921 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1981 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     2041 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2101 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     2161 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2221 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     2281 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2341 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagta gcggccgctg
     2401 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgtgtcc
     2461 cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc tgattagaaa
     2521 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     2581 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     2641 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     2701 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     2761 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
     2821 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     2881 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     2941 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     3001 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     3061 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     3121 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     3181 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     3241 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgag
     3301 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     3361 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     3421 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc
     3481 acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac
     3541 tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat
     3601 ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc
     3661 tattctgacc ttgccatcac gactgtgctg gtcattaaac gcgtattcag gctgaccctg
     3721 cgcgctgcgc agggctttat tgattccatt tttacactga tgaatgttcc gttgcgctgc
     3781 ccggattaca gctgaaagcg accaggtgct cggcgtggca agactcgcag cgaacccgta
     3841 gaaagccatg ctccagccgc ccgcattgga gaaattcttc aaattcccgt tgcacatagc
     3901 ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa
     3961 cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat
     4021 cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt
     4081 tacatacagt ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt
     4141 gctagacatt gccagccagt gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat
     4201 aactgtcacg cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca
     4261 aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt
     4321 atctaagaat aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg
     4381 cccaatatgc ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc
     4441 gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac
     4501 cccgcagaaa tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag
     4561 ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gcccggctca
     4621 tccttctggc gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca
     4681 tccattgccg ccatgagccg atcctccggc cactcgctgc tgttcacctt ggccaaaatc
     4741 atggccccca ccagcacctt gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt
     4801 gcccgcaccc gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag cttggccagc
     4861 cgatccgccg ccttgttgct ccccttaacc atcttgacac cccattgtta atgtgctgtc
     4921 tcgtaggcta tcatggaggc acagcggcgg caatcccgac cctactttgt aggggagggc
     4981 gcacttaccg gtttctcttc gagaaactgg cctaacggcc acccttcggg cggtgcgctc
     5041 tccgagggcc attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt
     5101 atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg gccaaggccg
     5161 actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg cacgccgaat
     5221 ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct gccgacctgt
     5281 atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga
     5341 ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgacc ggtgccgagc
     5401 gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac tgccacctga
     5461 tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt
     5521 acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc aagcccaagg
     5581 catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca ttagagcggg
     5641 ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc gagcgcctgc
     5701 ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc cgcaccgacc
     5761 gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac ttacaggaat
     5821 accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag aggcatcaac
     5881 gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc cgacgaagcc
     5941 cggcaggtca tgagccagac ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa
     6001 gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg ggaggtagcc
     6061 gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag
     6121 ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct gatcgcatcg
     6181 ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg gctgcgcttg
     6241 gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc aaggccatgg
     6301 gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac cggccagatg atgaaccggg
     6361 aatggtcagc cgccgaagtg ctccagaaca cgccatggct caagcggatg aatgcccagg
     6421 gcaatgacgt gtatatcagg cccgccgagc aggagcggca tggtctggtg ctggtggacg
     6481 acctcagcga gtttgacctg gatgacatga aagccgaggg ccgggagcct gccctggtag
     6541 tggaaaccag cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg
     6601 aacttcgggg gcagattgcc cggacgctgg ccagcgagta cgacgccgac ccggccagcg
     6661 ccgacagccg ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca
     6721 cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag accgccaccg
     6781 ctggcccggc gctggtgcag caggctggcc agcagatcga gcaggcccag cggcagcagg
     6841 agaaggcccg caggctggcc agcctcgaac tgcccgagcg gcagcttagc cgccaccggc
     6901 gcacggcgct ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg
     6961 acctcagcaa gtgcgacttt atcgccgcgc agaagctggc cagccggggc cgcagtgccg
     7021 aggaaatcgg caaggccatg gccgaggcca gcccagcgct ggcagagcgc aagcccggcc
     7081 acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc
     7141 ttgcgcgggc cgagctggca cgggcaccgg caccccgcca gcgaggcatg gacaggggcg
     7201 ggccagattt cagcatgtag tgcttgcgtt ggtactcacg cctgttatac tatgagtact
     7261 cacgcacaga agggggtttt atggaatacg aaaaaagcgc ttcagggtcg gtctacctga
     7321 tcaaaagtga caagggctat tggttgcccg gtggctttgg ttatacgtca aacaaggccg
     7381 aggctggccg cttttcagtc gctgatatgg ccagccttaa ccttgacggc tgcaccttgt
     7441 ccttgttccg cgaagacaag cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa
     7501 gaccaaaagg acaagcagac cggcgacctg ctggccagcc ctgacgctgt acgccaagcg
     7561 cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac
     7621 gacgaatacg aggcgctgcg cgagtgcctg gaagaactca gagcggcgca gggcgggggt
     7681 agtgaccccg ccagcgccta accaccaact gcctgcaaag gaggcaatca atggctaccc
     7741 ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg gactacgttt
     7801 tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt gccggtaaat
     7861 ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg
     7921 gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc accgccattc
     7981 atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg
     8041 acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc ccggagtggt
     8101 tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac acgctgcgcc
     8161 ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg
     8221 aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg
     8281 cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg gtactggtcg
     8341 ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc gaggaatggg
     8401 gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag gccaactatg
     8461 gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc aagcccgccg
     8521 tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac
     8581 agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct gttccgtgcc
     8641 ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta cggcgacggc
     8701 aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg catcctgcaa
     8761 gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc caagaccgaa
     8821 ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac cgctgatgcc
     8881 atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt
     8941 ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt atccatcatc
     9001 gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc cagcgacgag
     9061 gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt catgggtggc
     9121 ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga aaccgcccgc
     9181 ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa ggcttccata
     9241 gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc
     9301 cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac ggtaaccgag
     9361 ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc ccccccactc
     9421 tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct gctaattggt
     9481 aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa aattgcctac
     9541 tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccca c
//
