LOCUS       sequence-358664-        8109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector for expressing CFL-SN and GCaMP6f.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Goto A, Bota A, Miya K, Wang J, Tsukamoto S, Jiang X, Hirai D, 
            Murayama M, Matsuda T, McHugh TJ, Nagai T, Hayashi Y
  TITLE     Stepwise synaptic plasticity events drive the early phase of memory 
            consolidation.
  JOURNAL   Science. 2021 Nov 12;374(6569):857-863. doi: 
            10.1126/science.abj9195. Epub 2021 Nov 11.
  PUBMED    34762472
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abj9195"; journalName: "Science"; date: 
            "2021-11-12- 12"; volume: "374"; issue: "6569"; pages: "857-863"
COMMENT     Sequence Label: pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..8109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        210..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1122..1144
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1206..1225
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1292..1325)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1376..1409)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(1418..1861,1862..1867,1868..2302,2303..2320,
                     2321..2590,2591..2596,2597..2653,2654..2680,2681..2704,
                     2705..2707,2708..2740,2741..2743,2744..2761,2762..2770))
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     1977..1996
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2236..2257
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2363..2384)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      2665..2674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2795..2851)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(2867..3574,3575..3580,3581..3583))
                     /codon_start=1
                     /product="monomeric variant of the photosensitizing red 
                     fluorescent protein KillerRed (Takemoto et al., 2013)"
                     /label=SuperNova
                     /translation="MGSEVGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFN
                     VHAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDG
                     TMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPSETHMFPHGPNAVRQTA
                     TIGFTTADGGKMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQTRDTSDKRDHVCQREVA
                     YAHSVPRITSAIGSDED"
     protein_bind    4094..4127
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4178..4211
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4236..4824
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4289..4309)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4707..4718)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4856..5332
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5055..5074)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5372..5512
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5372..5501
                     /label=AAV2 ITR
     rep_origin      5587..6042
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5674..5693)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5884..5905
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6059..6078)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6178..6200
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6238..6256)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6324..6428
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6429..6497,6498..7289)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6647..6666)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7460..8048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7949..7968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccrggsgg ggsggggsgg
      661 ggsggggggs ggggsggggs grggcggrga ggkgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      841 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      901 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      961 tggctgcgtg aaagccttga ggggctccgg gagcgccggc aggaaggaaa tgggcgggga
     1021 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1081 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1141 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1201 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgcggcccaa
     1261 cggtaccgga tcctctagag tcgactccgg aataacttcg tataggatac tttatacgaa
     1321 gttatgcaga atggtagctg gattgtagct gctattagca atatgaaacc tcttaataac
     1381 ttcgtatagc atacattata cgaagttatg gcgcgcctca cttcgctgtc atcatttgta
     1441 caaactcttc gtagtttacc tgaccatccc catcgatgtc tgcttccctg atcatttcat
     1501 caacctcttc atctgttaac ttctctccaa ggtttgtcat cacgtggcga agctctgctg
     1561 cactgatgta gccattgcca tccttatcaa acacaccgaa cgcttctcta atttcttctt
     1621 ccgtgtccct gtatttcatt tttcttgcca tcattgtcag gaactcaggg aagtcgattg
     1681 tgccgtcacc gtcggcatct acttcattga tcatgtcctg cagctctgct tctgtggggt
     1741 tctgccccag agaccgcatc accgtcccca gctccttggt tgttattgtc ccatccccgt
     1801 ccttgtcaaa tagggagaat tcctctttaa attctgcgat ctgctcttca gtcagttggt
     1861 ccggcaggtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     1921 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     1981 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gatgtagcct tcgggcatgg
     2041 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     2101 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     2161 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc accctcgccg gacacgctga
     2221 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     2281 gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc tcgtccatgc
     2341 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     2401 tggggtcttt cgaaagtttg gactgcacgc tcaggtagtg gttgtcgggc agcagcacgg
     2461 ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc gagctgcacg ccgccgtcct
     2521 cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cttctgcttg tcggccttga
     2581 tatagacgtt ctcgagtgag ctcagccgac ctatagctct gactgcgtga cctgtcttat
     2641 tccacttacg acgtgatgag tcgaccatgg tggcgagatc cttatcgtca tcgtcgtaca
     2701 gatcccgacc catttgctgt ccaccagtca tgcttgccat accatgatga tgatgatgat
     2761 gagaacccat ggtgaccggt ggatcccggg atcctgggcc aggattctcc tcgacgtcac
     2821 ctgcttgttt caacagggag aagttagtgg ctccgcttcc ctcgagatcc tcgtcgctac
     2881 cgatggcgct ggtgatgcgg ggcacgctgt gggcgtaggc cacctcgcgc tggcacacgt
     2941 ggtcgcgctt gtcgctagtg tccctcgtct gcttggtgat gatggtcacg aagtgtgggc
     3001 cggggatctc gatggcgcgg ctgccgttga aggtcatctt gctgtcgaag tggcccatca
     3061 tcttgccgcc gtcggcggtg gtgaagccga tggtggcggt ctggcgcacg gcgttggggc
     3121 cgtgggggaa catgtgggtc tcgctgggca ggatgtccac cagctggtcg cgcatgatgg
     3181 ggccgtcggg ctggaagccg tcgcagttca cggtgatgcg gctcaccacg caggtgtcgt
     3241 ccagctcgta ggtgtggtgg ctggtcatgg tgccgtcgtt ctcgaagcgc acggtgcggt
     3301 cgatgctcag gccctcgggg aagcactcct gggcgaaatg gctgatgccg tcggggtagc
     3361 gggcgaagaa gggctcgccg tactggatca ggtggcagat gggcttccag ctcatgggca
     3421 gcttgccggt ctcgcacacg gcgtgcacgt tgaagtcgcc gtgggggaac ttgctgctgc
     3481 cgtcggccac gatggtgaac ttctggccgt tcacctcgcc gtcgatgaag attttgaagg
     3541 tcatgtcgct ctggaacagg gcggggccga cctctgaacc cattctagac aaaggcttgc
     3601 cctccaggga gatgacggca ctgcccccca gcttctctgc cagggtgcag cggtccttga
     3661 cctcctcgta gcagtttgct tgcaattcat gcttgatccc tgtcagcttc ttcttgatgg
     3721 cgtccttgga gctggcataa atcattttgc tcttaagtgg cgcagactcg ggggcccaga
     3781 agataaacac cagatcctcc ttcttgctct ccttggtctc ataggttgca tcatagaggg
     3841 catagcggca gtccttatct ggcagcatct tgacaaaggt ggcgtaggga tcgtcgacag
     3901 tctggcccac atcgcccacc aggatctcct tgccctcctc caggatgatg ttcttcttgt
     3961 cctcactcag gcagaagagc accgccttct tgcgcttctt cacctcctct ggcgttgaag
     4021 acttacgcac cttcatgtcg ttgaacacct tgatgacacc atcagagaca gccacaccgg
     4081 aggccatgct agcataactt cgtataaagt atcctatacg aagttatttg ccttaaccca
     4141 gaaattatca ctgttattct ttagaatggt gcaaagaata acttcgtata atgtatgcta
     4201 tacgaagtta tgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     4261 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4321 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4381 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4441 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4501 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4561 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4621 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
     4681 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4741 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4801 tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg
     4861 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
     4921 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
     4981 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
     5041 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
     5101 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
     5161 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
     5221 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
     5281 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
     5341 gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     5401 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     5461 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     5521 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     5581 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5641 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     5701 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5761 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     5821 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5881 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     5941 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     6001 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     6061 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     6121 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     6181 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     6241 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6301 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6361 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6421 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6481 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6541 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6601 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6661 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6721 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6781 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6841 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6901 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6961 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7021 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7081 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7141 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7201 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7261 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7321 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7381 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7441 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7501 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7561 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7621 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7681 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7741 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7801 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7861 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7921 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7981 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8041 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8101 ctcacatgt
//
