LOCUS       sequence-35868-e       15036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15036)
  AUTHORS   Beier KT, Saunders A, Oldenburg IA, Miyamichi K, Akhtar N, Luo L, 
            Whelan SP, Sabatini B, Cepko CL
  TITLE     Anterograde or retrograde transsynaptic labeling of CNS neurons with
            vesicular stomatitis virus vectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9. Epub 2011 Aug
            8.
  PUBMED    21825165
REFERENCE   2  (bases 1 to 15036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-09-13"; volume: "108(37)"; pages: 
            "15414-9. Epub 2011 Aug 8"
COMMENT     Sequence Label: pVSV YFP LCMV-G
FEATURES             Location/Qualifiers
     source          1..15036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(73..75,76..78,79..792)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(118..139)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(379..398)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     726..747
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(12104..12122)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12118..12165
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(12233..12251)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12234..12251)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12541..12560)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(12770..12789)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12889..12911
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12949..12967)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13035..13139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13140..13208,13209..14000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13358..13377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14171..14759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14660..14679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14913..14930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        15018..15036
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc
       61 agaattctcg agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      121 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      181 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      241 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
      301 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      361 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      421 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      481 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      541 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      601 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      661 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
      721 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      781 ctgtacaagt aatggccata tgaaaaaaac taacagtaat caaaatgtct gttacagtca
      841 agagaatcat tgacaacaca gtcatagttc caaaacttcc tgcaaatgag gatccagtgg
      901 aatacccggc agattacttc agaaaatcaa aggagattcc tctttacatc aatactacaa
      961 aaagtttgtc agatctaaga ggatatgtct accaaggcct caaatccgga aatgtatcaa
     1021 tcatacatgt caacagctac ttgtatggag cattaaagga catccggggt aagttggata
     1081 aagattggtc aagtttcgga ataaacatcg ggaaagcagg ggatacaatc ggaatatttg
     1141 accttgtatc cttgaaagcc ctggacggcg tacttccaga tggagtatcg gatgcttcca
     1201 gaaccagcgc agatgacaaa tggttgcctt tgtatctact tggcttatac agagtgggca
     1261 gaacacaaat gcctgaatac agaaaaaagc tcatggatgg gctgacaaat caatgcaaaa
     1321 tgatcaatga acagtttgaa cctcttgtgc cagaaggtcg tgacattttt gatgtgtggg
     1381 gaaatgacag taattacaca aaaattgtcg ctgcagtgga catgttcttc cacatgttca
     1441 aaaaacatga atgtgcctcg ttcagatacg gaactattgt ttccagattc aaagattgtg
     1501 ctgcattggc aacatttgga cacctctgca aaataaccgg aatgtctaca gaagatgtaa
     1561 cgacctggat cttgaaccga gaagttgcag atgaaatggt ccaaatgatg cttccaggcc
     1621 aagaaattga caaggccgat tcatacatgc cttatttgat cgactttgga ttgtcttcta
     1681 agtctccata ttcttccgtc aaaaaccctg ccttccactt ctgggggcaa ttgacagctc
     1741 ttctgctcag atccaccaga gcaaggaatg cccgacagcc tgatgacatt gagtatacat
     1801 ctcttactac agcaggtttg ttgtacgctt atgcagtagg atcctctgcc gacttggcac
     1861 aacagttttg tgttggagat aacaaataca ctccagatga tagtaccgga ggattgacga
     1921 ctaatgcacc gccacaaggc agagatgtgg tcgaatggct cggatggttt gaagatcaaa
     1981 acagaaaacc gactcctgat atgatgcagt atgcgaaaag agcagtcatg tcactgcaag
     2041 gcctaagaga gaagacaatt ggcaagtatg ctaagtcaga atttgacaaa tgaccctata
     2101 attctcagat cacctattat atattatgct acatatgaaa aaaactaaca gatatcatgg
     2161 ataatctcac aaaagttcgt gagtatctca agtcctactc tcgtctagat caggcggtag
     2221 gagagataga tgagatcgaa gcacaacgag ctgaaaagtc caattatgag ttgttccaag
     2281 aggacggagt ggaagagcat actaggccct cttattttca ggcagcagat gattctgaca
     2341 cagaatctga accagaaatt gaagacaatc aaggcttgta tgtaccagat ccggaagctg
     2401 agcaagttga aggctttata caggggcctt tagatgacta tgcagatgag gacgtggatg
     2461 ttgtattcac ttcggactgg aaacagcctg agcttgaatc cgacgagcat ggaaagacct
     2521 tacggttgac attgccagag ggtttaagtg gagagcagaa atcccagtgg cttttgacga
     2581 ttaaagcagt cgttcaaagt gccaaacact ggaatctggc agagtgcaca tttgaagcat
     2641 cgggagaagg ggtcatcata aaaaagcgcc agataactcc ggatgtatat aaggtcactc
     2701 cagtgatgaa cacacatccg taccaatcag aagccgtatc agatgtttgg tctctctcaa
     2761 agacatccat gactttccaa cccaagaaag caagtcttca gcctctcacc atatccttgg
     2821 atgaattgtt ctcatctaga ggagaattca tctctgtcgg aggtaacgga cgaatgtctc
     2881 ataaagaggc catcctgctc ggtctgaggt acaaaaagtt gtacaatcag gcgagagtca
     2941 aatattctct gtagactatg aaaaaaagta acagatatca caatctaagt gttatcccaa
     3001 tccattcatc atgagttcct taaagaagat tctcggtctg aaggggaaag gtaagaaatc
     3061 taagaaatta gggatcgcac caccccctta tgaagaggac actagcatgg agtatgctcc
     3121 gagcgctcca attgacaaat cctattttgg agttgacgag atggacacct atgatccgaa
     3181 tcaattaaga tatgagaaat tcttctttac agtgaaaatg acggttagat ctaatcgtcc
     3241 gttcagaaca tactcagatg tggcagccgc tgtatcccat tgggatcaca tgtacatcgg
     3301 aatggcaggg aaacgtccct tctacaaaat cttggctttt ttgggttctt ctaatctaaa
     3361 ggccactcca gcggtattgg cagatcaagg tcaaccagag tatcacgctc actgcgaagg
     3421 cagggcttat ttgccacata ggatggggaa gacccctccc atgctcaatg taccagagca
     3481 cttcagaaga ccattcaata taggtcttta caagggaacg attgagctca caatgaccat
     3541 ctacgatgat gagtcactgg aagcagctcc tatgatctgg gatcatttca attcttccaa
     3601 attttctgat ttcagagaga aggccttaat gtttggcctg attgtcgaga aaaaggcatc
     3661 tggagcgtgg gtcctggatt ctatcagcca cttcaaatga gctagtctag cttccagctt
     3721 ctgaacaatc cccggtttac tcagtctctc ctaattccag cctttcgaac aactaatatc
     3781 ctgtcttttc tatccctatg aaaaaaacta acagagatcg atctgtttcc ttgacacgcg
     3841 tcgcaccggg gatcctaggc tttttggatt gcgctttcct ctagatcaac tgggtgtcag
     3901 gccctatcct acagaaggat gggtcagatt gtgacaatgt ttgaggctct gcctcacatc
     3961 atcgatgagg tgatcaacat tgtcattatt gtgcttatcg tgatcacggg tatcaaggct
     4021 gtctacaatt ttgccacctg tgggatattc gcattgatca gtttcctact tctggctggc
     4081 aggtcctgtg gcatgtacgg tcttaaggga cccgacattt acaaaggagt ttaccaattt
     4141 aagtcagtgg agtttgatat gtcacatctg aacctgacca tgcccaacgc atgttcagcc
     4201 aacaactccc accattacat cagtatgggg acttctggac tagaattgac cttcaccaat
     4261 gattccatca tcagtcacaa cttttgcaat ctgacctctg ccttcaacaa aaagaccttt
     4321 gaccacacac tcatgagtat agtttcgagc ctacacctca gtatcagagg gaactccaac
     4381 tataaggcag tatcctgcga cttcaacaat ggcataacca tccaatacaa cttgacattc
     4441 tcagatcgac aaagtgctca gagccagtgt agaaccttca gaggtagagt cctagatatg
     4501 tttagaactg ccttcggggg gaaatacatg aggagtggct ggggctggac aggctcagat
     4561 ggcaagacca cctggtgtag ccagacgagt taccaatacc tgattataca aaatagaacc
     4621 tgggaaaacc actgcacata tgcaggtcct tttgggatgt ccaggattct cctttcccaa
     4681 gagaagacta agttcttcac taggagacta gcgggcacat tcacctggac tttgtcagac
     4741 tcttcagggg tggagaatcc aggtggttat tgcctgacca aatggatgat tcttgctgca
     4801 gagcttaagt gtttcgggaa cacagcagtt gcgaaatgca atgtaaatca tgatgccgaa
     4861 ttctgtgaca tgctgcgact aattgactac aacaaggctg ctttgagtaa gttcaaagag
     4921 gacgtagaat ctgccttgca cttattcaaa acaacagtga attctttgat ttcagatcaa
     4981 ctactgatga ggaaccactt gagagatctg atgggggtgc catattgcaa ttactcaaag
     5041 ttttggtacc tagaacatgc aaagaccggc gaaactagtg tccccaagtg ctggcttgtc
     5101 accaatggtt cttacttaaa tgagacccac ttcagtgatc aaatcgaaca ggaagccgat
     5161 aacatgatta cagagatgtt gaggaaggat tacataaaga ggcaggggag taccccccta
     5221 gcattgatgg accttctgat gttttccaca tctgcatatc tagtcagcat cttcctgcac
     5281 cttgtcaaaa taccaacaca caggcacata aaaggtggct catgtccaaa gccacaccga
     5341 ttaaccaaca aaggaatttg tagttgtggt gcatttaagg tgcctggtgt aaaaaccgtc
     5401 tggaaaagac gctgagcggc cgccctgcac aacagattct tcatgtttga accaaatcaa
     5461 cttgtgatat catgctcaaa gaggccttaa ttatatttta atttttaatt tttatgaaaa
     5521 aaactaacag caatcatgga agtccacgat tttgagaccg acgagttcaa tgatttcaat
     5581 gaagatgact atgccacaag agaattcctg aatcccgatg agcgcatgac gtacttgaat
     5641 catgctgatt acaatttgaa ttctcctcta attagtgatg atattgacaa tttgatcagg
     5701 aaattcaatt ctcttccgat tccctcgatg tgggatagta agaactggga tggagttctt
     5761 gagatgttaa catcatgtca agccaatccc atctcaacat ctcagatgca taaatggatg
     5821 ggaagttggt taatgtctga taatcatgat gccagtcaag ggtatagttt tttacatgaa
     5881 gtggacaaag aggcagaaat aacatttgac gtggtggaga ccttcatccg cggctggggc
     5941 aacaaaccaa ttgaatacat caaaaaggaa agatggactg actcattcaa aattctcgct
     6001 tatttgtgtc aaaagttttt ggacttacac aagttgacat taatcttaaa tgctgtctct
     6061 gaggtggaat tgctcaactt ggcgaggact ttcaaaggca aagtcagaag aagttctcat
     6121 ggaacgaaca tatgcaggat tagggttccc agcttgggtc ctacttttat ttcagaagga
     6181 tgggcttact tcaagaaact tgatattcta atggaccgaa actttctgtt aatggtcaaa
     6241 gatgtgatta tagggaggat gcaaacggtg ctatccatgg tatgtagaat agacaacctg
     6301 ttctcagagc aagacatctt ctcccttcta aatatctaca gaattggaga taaaattgtg
     6361 gagaggcagg gaaatttttc ttatgacttg attaaaatgg tggaaccgat atgcaacttg
     6421 aagctgatga aattagcaag agaatcaagg cctttagtcc cacaattccc tcattttgaa
     6481 aatcatatca agacttctgt tgatgaaggg gcaaaaattg accgaggtat aagattcctc
     6541 catgatcaga taatgagtgt gaaaacagtg gatctcacac tggtgattta tggatcgttc
     6601 agacattggg gtcatccttt tatagattat tacactggac tagaaaaatt acattcccaa
     6661 gtaaccatga agaaagatat tgatgtgtca tatgcaaaag cacttgcaag tgatttagct
     6721 cggattgttc tatttcaaca gttcaatgat cataaaaagt ggttcgtgaa tggagacttg
     6781 ctccctcatg atcatccctt taaaagtcat gttaaagaaa atacatggcc cacagctgct
     6841 caagttcaag attttggaga taaatggcat gaacttccgc tgattaaatg ttttgaaata
     6901 cccgacttac tagacccatc gataatatac tctgacaaaa gtcattcaat gaataggtca
     6961 gaggtgttga aacatgtccg aatgaatccg aacactccta tccctagtaa aaaggtgttg
     7021 cagactatgt tggacacaaa ggctaccaat tggaaagaat ttcttaaaga gattgatgag
     7081 aagggcttag atgatgatga tctaattatt ggtcttaaag gaaaggagag ggaactgaag
     7141 ttggcaggta gatttttctc cctaatgtct tggaaattgc gagaatactt tgtaattacc
     7201 gaatatttga taaagactca tttcgtccct atgtttaaag gcctgacaat ggcggacgat
     7261 ctaactgcag tcattaaaaa gatgttagat tcctcatccg gccaaggatt gaagtcatat
     7321 gaggcaattt gcatagccaa tcacattgat tacgaaaaat ggaataacca ccaaaggaag
     7381 ttatcaaacg gcccagtgtt ccgagttatg ggccagttct taggttatcc atccttaatc
     7441 gagagaactc atgaattttt tgagaaaagt cttatatact acaatggaag accagacttg
     7501 atgcgtgttc acaacaacac actgatcaat tcaacctccc aacgagtttg ttggcaagga
     7561 caagagggtg gactggaagg tctacggcaa aaaggatgga gtatcctcaa tctactggtt
     7621 attcaaagag aggctaaaat cagaaacact gctgtcaaag tcttggcaca aggtgataat
     7681 caagttattt gcacacagta taaaacgaag aaatcgagaa acgttgtaga attacagggt
     7741 gctctcaatc aaatggtttc taataatgag aaaattatga ctgcaatcaa aatagggaca
     7801 gggaagttag gacttttgat aaatgacgat gagactatgc aatctgcaga ttacttgaat
     7861 tatggaaaaa taccgatttt ccgtggagtg attagagggt tagagaccaa gagatggtca
     7921 cgagtgactt gtgtcaccaa tgaccaaata cccacttgtg ctaatataat gagctcagtt
     7981 tccacaaatg ctctcaccgt agctcatttt gctgagaacc caatcaatgc catgatacag
     8041 tacaattatt ttgggacatt tgctagactc ttgttgatga tgcatgatcc tgctcttcgt
     8101 caatcattgt atgaagttca agataagata ccgggcttgc acagttctac tttcaaatac
     8161 gccatgttgt atttggaccc ttccattgga ggagtgtcgg gcatgtcttt gtccaggttt
     8221 ttgattagag ccttcccaga tcccgtaaca gaaagtctct cattctggag attcatccat
     8281 gtacatgctc gaagtgagca tctgaaggag atgagtgcag tatttggaaa ccccgagata
     8341 gccaagtttc gaataactca catagacaag ctagtagaag atccaacctc tctgaacatc
     8401 gctatgggaa tgagtccagc gaacttgtta aagactgagg ttaaaaaatg cttaatcgaa
     8461 tcaagacaaa ccatcaggaa ccaggtgatt aaggatgcaa ccatatattt gtatcatgaa
     8521 gaggatcggc tcagaagttt cttatggtca ataaatcctc tgttccctag atttttaagt
     8581 gaattcaaat caggcacttt tttgggagtc gcagacgggc tcatcagtct atttcaaaat
     8641 tctcgtacta ttcggaactc ctttaagaaa aagtatcata gggaattgga tgatttgatt
     8701 gtgaggagtg aggtatcctc tttgacacat ttagggaaac ttcatttgag aaggggatca
     8761 tgtaaaatgt ggacatgttc agctactcat gctgacacat taagatacaa atcctggggc
     8821 cgtacagtta ttgggacaac tgtaccccat ccattagaaa tgttgggtcc acaacatcga
     8881 aaagagactc cttgtgcacc atgtaacaca tcagggttca attatgtttc tgtgcattgt
     8941 ccagacggga tccatgacgt ctttagttca cggggaccat tgcctgctta tctagggtct
     9001 aaaacatctg aatctacatc tattttgcag ccttgggaaa gggaaagcaa agtcccactg
     9061 attaaaagag ctacacgtct tagagatgct atctcttggt ttgttgaacc cgactctaaa
     9121 ctagcaatga ctatactttc taacatccac tctttaacag gcgaagaatg gaccaaaagg
     9181 cagcatgggt tcaaaagaac agggtctgcc cttcataggt tttcgacatc tcggatgagc
     9241 catggtgggt tcgcatctca gagcactgca gcattgacca ggttgatggc aactacagac
     9301 accatgaggg atctgggaga tcagaatttc gactttttat tccaagcaac gttgctctat
     9361 gctcaaatta ccaccactgt tgcaagagac ggatggatca ccagttgtac agatcattat
     9421 catattgcct gtaagtcctg tttgagaccc atagaagaga tcaccctgga ctcaagtatg
     9481 gactacacgc ccccagatgt atcccatgtg ctgaagacat ggaggaatgg ggaaggttcg
     9541 tggggacaag agataaaaca gatctatcct ttagaaggga attggaagaa tttagcacct
     9601 gctgagcaat cctatcaagt cggcagatgt ataggttttc tatatggaga cttggcgtat
     9661 agaaaatcta ctcatgccga ggacagttct ctatttcctc tatctataca aggtcgtatt
     9721 agaggtcgag gtttcttaaa agggttgcta gacggattaa tgagagcaag ttgctgccaa
     9781 gtaatacacc ggagaagtct ggctcatttg aagaggccgg ccaacgcagt gtacggaggt
     9841 ttgatttact tgattgataa attgagtgta tcacctccat tcctttctct tactagatca
     9901 ggacctatta gagacgaatt agaaacgatt ccccacaaga tcccaacctc ctatccgaca
     9961 agcaaccgtg atatgggggt gattgtcaga aattacttca aataccaatg ccgtctaatt
    10021 gaaaagggaa aatacagatc acattattca caattatggt tattctcaga tgtcttatcc
    10081 atagacttca ttggaccatt ctctatttcc accaccctct tgcaaatcct atacaagcca
    10141 tttttatctg ggaaagataa gaatgagttg agagagctgg caaatctttc ttcattgcta
    10201 agatcaggag aggggtggga agacatacat gtgaaattct tcaccaagga catattattg
    10261 tgtccagagg aaatcagaca tgcttgcaag ttcgggattg ctaaggataa taataaagac
    10321 atgagctatc ccccttgggg aagggaatcc agagggacaa ttacaacaat ccctgtttat
    10381 tatacgacca ccccttaccc aaagatgcta gagatgcctc caagaatcca aaatcccctg
    10441 ctgtccggaa tcaggttggg ccaattacca actggcgctc attataaaat tcggagtata
    10501 ttacatggaa tgggaatcca ttacagggac ttcttgagtt gtggagacgg ctccggaggg
    10561 atgactgctg cattactacg agaaaatgtg catagcagag gaatattcaa tagtctgtta
    10621 gaattatcag ggtcagtcat gcgaggcgcc tctcctgagc cccccagtgc cctagaaact
    10681 ttaggaggag ataaatcgag atgtgtaaat ggtgaaacat gttgggaata tccatctgac
    10741 ttatgtgacc caaggacttg ggactatttc ctccgactca aagcaggctt ggggcttcaa
    10801 attgatttaa ttgtaatgga tatggaagtt cgggattctt ctactagcct gaaaattgag
    10861 acgaatgtta gaaattatgt gcaccggatt ttggatgagc aaggagtttt aatctacaag
    10921 acttatggaa catatatttg tgagagcgaa aagaatgcag taacaatcct tggtcccatg
    10981 ttcaagacgg tcgacttagt tcaaacagaa tttagtagtt ctcaaacgtc tgaagtatat
    11041 atggtatgta aaggtttgaa gaaattaatc gatgaaccca atcccgattg gtcttccatc
    11101 aatgaatcct ggaaaaacct gtacgcattc cagtcatcag aacaggaatt tgccagagca
    11161 aagaaggtta gtacatactt taccttgaca ggtattccct cccaattcat tcctgatcct
    11221 tttgtaaaca ttgagactat gctacaaata ttcggagtac ccacgggtgt gtctcatgcg
    11281 gctgccttaa aatcatctga tagacctgca gatttattga ccattagcct tttttatatg
    11341 gcgattatat cgtattataa catcaatcat atcagagtag gaccgatacc tccgaacccc
    11401 ccatcagatg gaattgcaca aaatgtgggg atcgctataa ctggtataag cttttggctg
    11461 agtttgatgg agaaagacat tccactatat caacagtgtt tggcagttat ccagcaatca
    11521 tttccgatta ggtgggaggc tatttcagta aaaggaggat acaagcagaa gtggagtact
    11581 agaggtgatg ggctcccaaa agatacccga atttcagact ccttggcccc aatcgggaac
    11641 tggatcagat ctttggaatt ggtccgaaac caagttcgtc taaatccatt caataagatc
    11701 ttgttcaatc agctatgtcg tacagtggat aatcatttga agtggtcaaa tttgcgaaaa
    11761 aacacaggaa tgattgaatg gatcaatggg cgaatttcaa aagaagaccg gtctatactg
    11821 atgttgaaga gtgacctaca tgaggaaaac tcttggagag attaaaaaat caggaggaga
    11881 ctccaaactt taagtatgaa aaaaactttg atccttaaga ccctcttgtg gtttttattt
    11941 ttttatctgg ttttgtggtc ttcgtgggtc ggcatggcat ctccacctcc tcgcggtccg
    12001 acctgggcat ccgaaggagg acgtcgtcca ctcggatggc taagggagag ctcggatccg
    12061 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
    12121 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
    12181 atatccggat cgagatcctc tagagtcgac ctgcaggcat gcaagcttgt attctatagt
    12241 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
    12301 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
    12361 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
    12421 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
    12481 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
    12541 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
    12601 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
    12661 ccttgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
    12721 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
    12781 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
    12841 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
    12901 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
    12961 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    13021 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    13081 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    13141 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    13201 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    13261 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    13321 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    13381 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    13441 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    13501 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    13561 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    13621 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    13681 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    13741 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    13801 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    13861 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    13921 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    13981 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    14041 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    14101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    14161 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    14221 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    14281 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    14341 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    14401 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    14461 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    14521 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    14581 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    14641 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    14701 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    14761 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    14821 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    14881 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    14941 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaggggga
    15001 tctcgatccc gcgaaattaa tacgactcac tatagg
//
