LOCUS       sequence-358685-        9880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid that directs the expression of NGLY1 in mammalian
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Forcina GC, Pope L, Murray M, Dong W, Abu-Remaileh M, Bertozzi CR, 
            Dixon SJ
  TITLE     Ferroptosis regulation by the NGLY1/NFE2L1 pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2118646119. doi: 
            10.1073/pnas.2118646119. Epub 2022 Mar 10.
  PUBMED    35271393
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-03-15"; volume: "119(11):e2118646119. 
            doi"; pages: " 10.1073/pnas.2118646119. Epub 2022 Mar 10"
COMMENT     Sequence Label: pLenti-CMV-NGLY1-Puro
FEATURES             Location/Qualifiers
     source          1..9880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5130..5718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5183..5203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5601..5612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5732..6231
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5767..5790)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6164..6183
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6252..6851
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6252..6271)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6748..6768
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6989..7222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7294..7415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7331..7350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7385..7404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7455..7590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7610..7629)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7611..7629)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7639..7656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7639..7655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7648..7670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7797..8252
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7884..7903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8094..8115
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8278..8382
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8383..8451,8452..9243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8601..8620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9414..9880,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat ggcggcggcg gcattgggca gctcctcagg ctcggcgtcc
     3181 ccggccgtgg ctgagctctg ccagaacacc ccggagacct ttttggaggc ctccaagctg
     3241 ctgctcacct atgctgacaa catcctcaga aaccctaatg atgaaaaata tagatccatc
     3301 cggattggaa acacagcctt ttctactaga ctcttgcctg tcagaggagc tgttgaatgt
     3361 ttatttgaaa tgggctttga agagggagaa acacatctca tctttcctaa aaaagcttca
     3421 gtggagcagc tgcaaaaaat tcgtgacctg attgccatag agagaagtag cagactggat
     3481 ggctcaaata agagccacaa agtaaagtca tctcagcaac ctgcagccag tacccagctt
     3541 cctacaacac catcttcaaa tcccagtggg ttaaaccagc acacaaggaa ccgtcaaggg
     3601 cagtcatcag atccaccatc tgcttcaacg gttgctgctg actcagccat tctagaagtt
     3661 cttcagtcca acattcagca tgtgctggtc tatgaaaatc ctgctcttca ggagaaagcg
     3721 ttggcttgta ttccggtcca agaactaaaa aggaaatcac aagaaaagtt atcgagagct
     3781 agaaaattgg ataaaggtat caatataagt gatgaggatt ttcttttgct ggagcttttg
     3841 cactggttta aggaagaatt ttttcactgg gtgaataacg ttttgtgcag caaatgtggt
     3901 ggacagacta ggtctagaga tagatcatta ctgcccagtg atgatgagct gaagtggggt
     3961 gcaaaggaag tggaagatca ttactgtgat gcctgccagt tcagcaatcg attcccaaga
     4021 tataataacc ctgagaaact tttggaaaca agatgtggac ggtgtggcga gtgggccaat
     4081 tgttttacac tgtgctgccg agctgtaggg tttgaagctc gctatgtttg ggattacaca
     4141 gaccatgtct ggacagaagt ctattctcct tctcagcagc ggtggctgca ctgtgatgca
     4201 tgtgaagatg tctgtgacaa gccactcctt tatgaaatag gatggggcaa gaagctttcc
     4261 tatgtcatag cattttcaaa agatgaggta gttgatgtca cttggcgata ttcctgcaaa
     4321 catgaagagg tgattgccag aagaactaag gttaaagaag cattacttcg agacactatt
     4381 aatgggctta ataagcagag gcaactgttt ttgtcagaaa acagaaggaa agaacttctc
     4441 cagaggataa ttgtggagct tgttgaattt atatctccca aaacccctaa acctggagaa
     4501 cttgggggaa gaatatctgg gtcagtggct tggagagtag cccgaggtga aatgggtcta
     4561 cagagaaaag aaaccttgtt tattccctgt gaaaatgaga agatttctaa acagctccac
     4621 ctttgttaca atattgtgaa agatcgttat gttcgagttt caaataacaa tcaaaccatt
     4681 tctggatggg agaatggcgt gtggaaaatg gaatctatat tcagaaaagt tgaaacagac
     4741 tggcacatgg tatatttggc ccgaaaggaa ggatcatctt ttgcttatat ttcctggaag
     4801 tttgagtgtg ggtcagttgg cctaaaagta gatagcattt ctattagaac aagtagtcaa
     4861 acttttcaga ctggaacagt agaatggaaa ttgcgatctg atacagcaca agtagaactg
     4921 acaggcgata acagtcttca ctcctatgct gatttttctg gtgccactga agttattttg
     4981 gaagcagaat taagcagagg agatggtgat gtcgcttggc aacacaccca gctgtttaga
     5041 caaagcttaa atgaccatga agaaaattgt ttggagataa ttataaaatt cagtgacctt
     5101 taaatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     5161 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5221 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5281 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5341 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5401 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5461 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5521 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5581 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5641 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5701 tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag
     5761 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     5821 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     5881 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     5941 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     6001 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     6061 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     6121 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     6181 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     6241 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6301 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6361 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6421 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6481 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6541 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6601 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6661 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6721 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6781 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6841 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     6901 atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6961 agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa
     7021 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7081 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7141 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7201 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7261 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7321 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7381 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7441 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7561 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     7621 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7681 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7741 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     7801 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7861 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7921 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7981 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8041 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8101 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8161 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8221 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8401 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8461 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8521 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8581 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     8641 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8701 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8761 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8821 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8881 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8941 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9001 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9061 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9121 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9181 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9541 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9841 gcggacaggt atccggtaag cggcagggtc ggaacaggag
//
