LOCUS       sequence-358695-        2535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For gateway cloning of N-terminal tagged TARG1 constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Zaja R, Aydin G, Lippok BE, Feederle R, Luscher B, Feijs KLH
  TITLE     Comparative analysis of MACROD1, MACROD2 and TARG1 expression, 
            localisation and interactome.
  JOURNAL   Sci Rep. 2020 May 19;10(1):8286. doi: 10.1038/s41598-020-64623-y.
  PUBMED    32427867
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-05-19"; volume: "10(1):8286. doi"; pages: " 
            10.1038/s41598-020-64623-y"
COMMENT     Sequence Label: pDONR/zeo-TARG1
FEATURES             Location/Qualifiers
     source          1..2535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(389..475)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     524..546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     538..555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     539..555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    571..670
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1133..1232)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1249..1268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1250..1268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1273..1289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1273..1289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1357..1731)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1750..1797)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      1887..2475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2376..2395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
      241 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      301 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
      361 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
      421 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
      481 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
      541 aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg
      601 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      661 aaagcaggct tcatggccag cagccttaat gaagatccag aaggaagcag aatcacttat
      721 gtgaaaggag acctttttgc atgcccgaaa acagactctt tagcccactg tatcagtgag
      781 gattgtcgca tgggcgctgg gatagctgtc ctctttaaga agaaatttgg aggggtgcaa
      841 gaacttttaa atcaacaaaa gaaatctgga gaagtggctg ttctgaagag agatgggcga
      901 tatatatatt acttgattac aaagaaaagg gcttcgcaca agccaactta tgaaaactta
      961 cagaagagtt tagaggcaat gaagtctcat tgtctgaaga atggagtcac tgacctctcc
     1021 atgcccagga ttggatgtgg tcttgatcgt ctgcaatggg aaaatgtatc tgcgatgatc
     1081 gaggaggtat ttgaggcaac agacatcaaa attactgtgt acacactctg agacccagct
     1141 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1201 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     1261 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1321 tgttacattg cacaagataa aaatatatca tcatgatcag tcctgctcct cggccacgaa
     1381 gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc
     1441 gatctcggtc atggccggcc cggaggcgtc ccggaagttc gtggacacga cctccgacca
     1501 ctcggcgtac agctcgtcca ggccgcgcac ccacacccag gccagggtgt tgtccggcac
     1561 cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca caccggcgaa
     1621 gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg gtccagaact cgaccgctcc
     1681 ggcgacgtcg cgcgcggtga gcaccggaac ggcactggtc aacttggcca tggtttagtt
     1741 cctcaccttg tcgtattata ctatgccgat atactatgcc gatgattaat tgtcaacacg
     1801 tgctgatcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     1861 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1921 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1981 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2041 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2101 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2161 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2221 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2281 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2341 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2401 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2461 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2521 ccttttgctc acatg
//
