LOCUS       sequence-358696-        3354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For gateway cloning of N-terminal tagged MACROD2 constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Zaja R, Aydin G, Lippok BE, Feederle R, Luscher B, Feijs KLH
  TITLE     Comparative analysis of MACROD1, MACROD2 and TARG1 expression, 
            localisation and interactome.
  JOURNAL   Sci Rep. 2020 May 19;10(1):8286. doi: 10.1038/s41598-020-64623-y.
  PUBMED    32427867
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-05-19"; volume: "10(1):8286. doi"; pages: " 
            10.1038/s41598-020-64623-y"
COMMENT     Sequence Label: pDONR/zeo-MACROD2
FEATURES             Location/Qualifiers
     source          1..3354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(389..475)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     524..546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     538..555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     539..555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    571..670
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1952..2051)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2068..2087)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2069..2087)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2092..2108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2092..2108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(2176..2550)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2569..2616)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      2706..3294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3195..3214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
      241 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      301 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
      361 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
      421 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
      481 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
      541 aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg
      601 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      661 aaagcaggct tcatgtaccc cagcaacaag aagaaaaagg tgtggagaga ggagaaagaa
      721 cgtttattga agatgacctt agaagagaga cgcaaagaat acctaagaga ctatattccc
      781 ctgaacagca ttctgtcatg gaaggaggag atgaagggca agggccaaaa tgatgaagaa
      841 aatactcagg aaacatccca ggtgaagaaa agtttgactg aaaaagtttc tctctataga
      901 ggtgacatca cattgctaga ggtagatgct atagtcaatg ccgcaaatgc cagtcttctt
      961 ggaggaggag gtgtggatgg ctgtattcat agagcagccg gcccctgttt gctagctgaa
     1021 tgtcgtaacc tgaatggctg tgatactgga catgcaaaaa tcacatgtgg ctatgacctt
     1081 cctgcaaaat atgtcatcca tactgtaggg ccaatagcca ggggccatat taatggttcc
     1141 cacaaggaag accttgcaaa ttgctataaa tcatctctga agctcgtgaa agaaaataac
     1201 atccgatcag ttgcatttcc ctgcatctca acaggcattt atggctttcc caacgagcct
     1261 gctgcagtca ttgccctcaa caccattaag gaatggcttg ccaagaatca ccatgaggtg
     1321 gatcggatca ttttctgtgt cttcttagaa gttgacttca aaatctacaa aaagaaaatg
     1381 aatgagtttt tctccgtaga cgataataat gaagaagaag aggatgttga aatgaaagaa
     1441 gattcagatg agaacggtcc agaggagaag caaagtgtgg aagaaatgga agagcagagc
     1501 caagatgcag atggtgtcaa cactgtcact gtgcccggcc ctgcttcaga agaggcagtt
     1561 gaagactgta aagatgaaga ttttgcaaag gatgaaaata ttacaaaagg cggtgaagtg
     1621 acagatcatt ctgtgcgtga ccaagatcat cccgatggac aagagaatga ttcaacgaag
     1681 aatgaaataa aaattgaaac agaatcgcag agctcatata tggaaacaga agaactttca
     1741 tcaaaccaag aagatgccgt gattgtggag caaccagaag tgattccatt aacagaggac
     1801 caagaagaaa aagaaggtga aaaagctcca ggcgaggaca cacctaggat gcctgggaaa
     1861 agtgaaggct ccagtgacct agaaaatact ccaggtcctg atgcaggggc acaagatgaa
     1921 gcgaaggaac aaagaaatgg aactaaatga gacccagctt tcttgtacaa agttggcatt
     1981 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2041 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2101 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2161 aatatatcat catgatcagt cctgctcctc ggccacgaag tgcacgcagt tgccggccgg
     2221 gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg atctcggtca tggccggccc
     2281 ggaggcgtcc cggaagttcg tggacacgac ctccgaccac tcggcgtaca gctcgtccag
     2341 gccgcgcacc cacacccagg ccagggtgtt gtccggcacc acctggtcct ggaccgcgct
     2401 gatgaacagg gtcacgtcgt cccggaccac accggcgaag tcgtcctcca cgaagtcccg
     2461 ggagaacccg agccggtcgg tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag
     2521 caccggaacg gcactggtca acttggccat ggtttagttc ctcaccttgt cgtattatac
     2581 tatgccgata tactatgccg atgattaatt gtcaacacgt gctgatcatg accaaaatcc
     2641 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2821 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3061 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3121 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3181 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3241 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3301 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catg
//
