LOCUS       sequence-358730-       10400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli 
            BL21(DE3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Schwaiger KN, Voit A, Wiltschi B, Nidetzky B
  TITLE     Engineering cascade biocatalysis in whole cells for bottom-up 
            synthesis of cello-oligosaccharides: flux control over three 
            enzymatic steps enables soluble production.
  JOURNAL   Microb Cell Fact. 2022 Apr 9;21(1):61. doi: 
            10.1186/s12934-022-01781-w.
  PUBMED    35397553
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-04-9"; volume: "21(1):61. doi"; pages: " 
            10.1186/s12934-022-01781-w"
COMMENT     Sequence Label: pPOLY_new
FEATURES             Location/Qualifiers
     source          1..10400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..29
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10..28
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    29..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             69..89
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             2608..2619
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2638..2655
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             5629..5640
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5656..5679
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      complement(7207..7254)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      7261..7355
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    7379..7519
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7434..7453
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7534..7551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7705..8293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7785..7804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8352..9143,9144..9212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8975..8994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     RBS             9219..9230
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(9240..10322)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10284..10303
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10323..10400)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 gaattcagtt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
       61 aaataatgtt gtttaacttt aagaaggaga tatcatatgg gcagcagcca tcaccatcac
      121 catcacgggt cgacccgcta tggtcatttt gacgatgaag cacgcgagta cgttatcacc
      181 acaccgcata ctccgtaccc ttggatcaac tatctgggtt cggaacagtt cttcagtctt
      241 ttgtcgcacc aggctggcgg ctattcgttt tatcgcgatg ccaagatgcg tagattgaca
      301 agatacagat acaacaacat accagcagac gcgggaggcc ggtatcttta tgtaaacgac
      361 ggtggggacg tttggacgcc atcgtggctt cctgttaaag cagacttaga tcattttgaa
      421 gccagacatg gtcttggcta cagtaccatt acaggtgaaa gaaatggggt acgggttgag
      481 accttgtttt tcgtcccagt cggtgagaac gcggaggtcc agaaagttac ggtcactaat
      541 acctctgact cgtataaatc cctgacgttg ttttcttttg tcgaattttg tctttggaac
      601 gcgcaagacg atcaaactaa ctaccagcgt aatctgagca taggagaggt tgaagtggaa
      661 caagaatctc cgcatggcag tgcaatttat caccgtacag agtaccggga gagacgcgat
      721 cactacgcgg tattcgccgt taatacgcag gccgagggtt tcgatacaga tcgcgacaca
      781 tttgtgggag cctataactc gcttggtgaa gccgcagttc cccttaaggg agaaagtgca
      841 aatagcgttg caagtggatg gtatcctatt ggctctcata gcgtggcagt gtctttagcg
      901 cctggagaat ccagagaatt ggtttatgtg cttgggtacg tggaaaatcc tgatgaggag
      961 aagtgggcag acgacgcaaa acaggtcgtg aataaagaaa gagctcacgc tttattatca
     1021 agatttgcta cgtccgaaca gactgacgca gctttcgccg ctttgaagga ttattggacg
     1081 gatttgttat cgacgtattc cgttagttcc aatgacgaaa aattggaccg gatggttaat
     1141 atatggaatc agtaccaatg catggtaacc tttaacatga gtcgctcggc gagctttttc
     1201 gagaccggta ttgggagagg gatggggttt cgtgatagca accaagacct tctgggcttt
     1261 gttcacttaa tcccagagcg ggcgcgtgag cgcatcattg acatagcgtc aacccagttc
     1321 gcggacggtt ccgcttatca ccaataccaa cctttaacga agcgcggtaa caacgacatt
     1381 ggctctgggt ttaacgacga tcctctttgg ttaatcgcgg gaactgccgc ctacatcaaa
     1441 gaaactggtg acttctccat attagatgag cccgtacctt ttgataacga accggggtca
     1501 gaagttccgt tatttgagca cctgacccgt agttttgagt ttaccgtaac ccatcggggt
     1561 ccccacggtt tgcctcttat tggccgcgca gattggaacg attgtttgaa tcttaattgc
     1621 ttcagtacaa ccccgggcga gtcatttcag acaacggaga accaagccgg tggcgtagcg
     1681 gagtcaacgt tcatagccgc gcaattcgtt ttatatgggg agcagtatgc cgagttggca
     1741 gcgcggcgcg ggttggccga cgtagccgat agagcaagag ggcatgtggc tgaaatgaga
     1801 gacgcacttc tgaccgacgg gtgggacggc agctggttct tgagagctta tgactattat
     1861 ggaaacccta tcggcaccga cgcacatgac gaagggaaga tttggatcga accgcaaggg
     1921 ttcgcggtga tggctggagt cggtgtaggc gaagggcctc aagatacgga cgctcccgct
     1981 attaaggcgc ttgattctgt gaacgaaatg ttggcaaccg atcacggtat ggtccttcaa
     2041 tacccagcat acactacata ccaagtacac atgggtgaag tatccactta tccacccggg
     2101 tataaagaga atggaggtat attttgccac aataatcctt gggttatcat agcggaaact
     2161 gtcgtcggaa gaggcggacg ggcatttgat tactacaaga gaattactcc cgcgtatcgc
     2221 gaggacataa gcgacgttca ccgtcttgaa ccctatgtct atgcacaaat gatagcaggg
     2281 aaggaggcgg tccgtcacgg tgaagcaaag aacagctggc ttaccgggac tgcggcttgg
     2341 aactttgtga ccgtgtcaca gtaccttctt ggggttcgcc cagagtacga cggcttggtg
     2401 gtagacccgc agatcggacc tgacgtacct agctttacgg ttacgcgtgt cgcccgtgga
     2461 gccacttacg agataacagt cactaacagt gggaccgacg gatcacgcgg tcgccttgtc
     2521 gttgatggaa cacctgtaga aggcaacttg gttccttatg cccccgcagg gtcaacagtt
     2581 cgtgtcgatg tgacattgta gtgtacaaaa gaggagaaaa ctagtatgag aggatcgcat
     2641 caccatcacc atcacggatc cattaccaaa gtgaccccgc gtagctgcaa actgaccccg
     2701 ttcgagattc tgaaacaaaa attcggtagc attattgcga tgagcggctg cgcggatgcg
     2761 atctacagcg acagcagctg caagaacgtg atcggtatta ccggcctgcc gatcaaagcg
     2821 ccggtgattc aggttctgct ggagaacagc aagctggcgg cgttcctgaa cgaatacgtg
     2881 caaaacaaaa gcgtttgcct ggcgctggag ggtatgcgtt attgcctggt gtttgaagac
     2941 ggccagaagg ttaaactgcc gcaagagctg gacgatatca ttcagagcac cgtgagcgat
     3001 gttaaaaact gggcgggtac cctgaccgag aacggcgaac acgtgatcga cctgaagaaa
     3061 ccgagcccgg gtccgcactt ctacgttaac ctgctgattg gtaaccgtct gggctttaac
     3121 cgtaccctgc aaaccacccc gaagagcgtg gttgatcgtt tcggtcgtgg cagctttcgt
     3181 agccatgcgg cgacccaggt tctggcgacc cgttacgaca tgcgtcaaga ggaaaacggc
     3241 ttcccggcga accgtcagtt ttacctgtat gagaacggca agcaaatctt ctacagcggc
     3301 agcgtggacg atgacaacat cctggaagcg aaatgcattc acagctgcaa ccgtaccgtt
     3361 attaagtata aaaccgcgtg caagctggag atcacccgta ccatttttct gtgcccgcag
     3421 aagaaaggtc tgccgctggc gaccgagatc caaatcatca acatcaagaa caacagcgaa
     3481 cgtaaacgta acctgagcat cacctacacc ggcatgtttg gtaccgcggc gacccacgcg
     3541 atctttgaag atgtgaccta caccaacgtt attatgcaga gcgcggcgct gtataacgac
     3601 aacggcgagt tcatcggcat taccccggat tactatcgta agaaatacaa cgaagacacc
     3661 cgtttcgtga ccatgtttgt tcgtaaggac ggtaaacgta tcttcccgaa gagcttctgc
     3721 accgattata acgactttgt gggtaacggt accctggagc acccggcggg tggcgaacac
     3781 ctgaacaaca agctgaaccg taaaggtccg ggcttctttg cgctgggtac cccgttcacc
     3841 attatgccgg gccaagacat cgagattgat acctttaccg gtatgagcag cagcaaggat
     3901 aacccgaact acgatgacac caccatgccg aaagagctgg aagcgctgct gcgttatttc
     3961 gaagcgagcg atagcgtgca gagcgcgctg cgtgaggtta tcgacttcca agaaaagtac
     4021 tgcaagtaca tcgagttcaa gaccgaagac aagatgttca acgcgggttt taacaaaaac
     4081 ctggcgtttc agatcctgta ccaaaccttc atgagccgta gcttttgcca gacccaaaag
     4141 ggttatcgtg agatcggctt ccgtgaaatt caggatctgt acgcgagcat gtactacttc
     4201 atcaacctgg gttaccaaga cttcgtgaag aacctgctgt ttgagtggac cagcaacgtt
     4261 tacaaaatgg gctatgcgaa ccacaacttc tactgggttg gtaaacagcc gggcctgtat
     4321 agcgatgaca gcctgtggct gctgcaagcg tactatcgtt acatcattta taccaaggat
     4381 accagcatcc tgaacgagga agtgccggtt gcggatggta acaacgaaaa gcgtgcggtg
     4441 cgtgacaccc tgaaagcgat cattcagtac agcgcgttca tcagcgttgg taaccatggt
     4501 ctgccgctgc tggatctggc ggactggaac gattgcctga agattgataa cgactatgtg
     4561 gacggtgcgc agaaggagaa actgtactat aagcaactga aagaaaccgg tggcaaatac
     4621 ggcgatcgtt tcatgagcga ctatagcgag agcgttatga acgcgtttct gctgaaggtg
     4681 gcggttgatg acctggcgga gatcgcggaa ctggataagg acgcggagct ggaacagagc
     4741 atgcgtaaaa tcagcagcgc gctgagcgaa aagattcaaa aatacgcgtg gaagggtaac
     4801 ttctttgcgc gtgtgctgct gaaccgttac aaagatggca gcttcaccta tgttggtgcg
     4861 aagggcgaca aactgagcgc ggacccgaac atcgatggta cctatttcct gaacagcttt
     4921 agctgggcga ttctgagcgg tgtggcgacc gatgagcaga tcggcattat ggttgacacc
     4981 ctgaagaaat acatgctgac cccgtatggc atccgtctga ccaccccggc ggatctgaac
     5041 aaactggcga acgacaccgc gaccgatcac tacttctatg gtgaccgtga aaacggcgcg
     5101 gtgtttaagc atgcgagcat gatggcgacc gcggcgctgg cgaaggcggc gaaaagcgtt
     5161 aaggataaag cgctggcgaa agagatggcg cgtctggcgt actatatgat cgatctggtg
     5221 ctgccgtaca agaacctgga agacccgttc aaactggcgg gtaacccgcg tatctgcacc
     5281 cagtatatta acgcggacag cggcgagaac attggtccgc tgctgagcgg taccgcgacc
     5341 tggctgaacc tgaccatcac cagcctggcg ggtattgagt acgtgaagga tggcattatg
     5401 ttcagcccgg ttctgcgtga ggacgaaacc cgtctggaat acagcctgaa ggcgccgggt
     5461 tgcagctata acatcaccat taccaaaccg cgtggctttg cgcgtctgat cgacagcgcg
     5521 tacgatctgc gtattgacgg taagaaaacc gacaactgca ttatcccgat gttcaccgat
     5581 ggtaaagagc atagcgtgac cctggagttc aagtaacaat tgtgtacaaa agaggagaaa
     5641 acccccatgg caagctggag ccacccgcag ttcgaaaagg gtaccatgaa aaataaagtc
     5701 caactgatta cctatgcgga tcgtctgggt gatggcacca ttaaaagcat gacggacatc
     5761 ctgcgtaccc gctttgatgg cgtttatgac ggtgtccaca ttctgccgtt tttcaccccg
     5821 tttgatggcg ccgacgcagg tttcgatccg atcgaccata ccaaagttga tgaacgtctg
     5881 ggtagctggg atgacgtcgc cgaactgtct aaaacccaca acattatggt ggatgcaatc
     5941 gttaatcaca tgtcatggga atcgaaacag ttccaagatg ttctggccaa aggcgaagaa
     6001 tcagaatatt acccgatgtt tctgaccatg agcagcgtgt tcccgaacgg tgccacggaa
     6061 gaagacctgg caggcattta tcgtccgcgt ccgggtctgc cgtttaccca ctacaaattc
     6121 gcaggcaaaa cgcgtctggt ctgggtgtct tttaccccgc agcaagtgga catcgatacg
     6181 gacagtgata aaggttggga atatctgatg tccattttcg accaaatggc ggccagccat
     6241 gtgtcttata tccgcctgga tgcggttggc tacggtgcca aagaagcagg caccagttgc
     6301 tttatgaccc cgaaaacgtt caaactgatt tcccgtctgc gcgaagaagg cgtgaaacgc
     6361 ggtctggaaa ttctgatcga agtccacagc tattacaaaa aacaggtgga aatcgcgtct
     6421 aaagtggatc gcgtttatga ctttgccctg ccgccgctgc tgctgcatgc cctgagtacc
     6481 ggccacgtcg aaccggtggc ccattggacg gatattcgtc cgaacaatgc tgttaccgtc
     6541 ctggatacgc atgacggcat cggcgttatt gatatcggtt cagatcagct ggaccgctcg
     6601 ctgaaaggtc tggtgccgga cgaagatgtg gataacctgg tgaatacgat tcatgcgaac
     6661 acccacggcg aatcacaggc agctaccggt gcggcggcat cgaacctgga tctgtaccaa
     6721 gttaatagta cctactactc cgctctgggc tgtaacgatc agcactacat cgctgcgcgt
     6781 gcggtgcagt ttttcctgcc gggtgttccg caagtctatt acgtgggcgc cctggcaggt
     6841 aaaaatgata tggaactgct gcgcaaaacc aacaatggtc gtgacattaa ccgccattat
     6901 tacagcacgg ccgaaatcga tgaaaatctg aaacgtccgg tggttaaagc actgaacgct
     6961 ctggcgaaat ttcgcaatga actggatgct tttgacggca ccttctcata taccacggat
     7021 gacgatacga gtatttcctt tacctggcgt ggtgaaacgt cgcaggccac cctgacgttc
     7081 gaaccgaaac gcggcctggg tgttgataat accacgccgg tcgcaatgct ggaatgggaa
     7141 gacagtgctg gtgaccatcg ctccgacgac ctgatcgcta atccgccggt tgttgcgtaa
     7201 aagcttcaaa aaacccctca agacccgttt agaggcccca aggggttatg ctaggagctc
     7261 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     7321 tcggttgccg ccgggcgttt tttattggtg agaatggatc cgttggcggg tgtcggggcg
     7381 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     7441 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     7501 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7561 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7621 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7681 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7741 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7801 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7861 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7921 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8041 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8101 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8161 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8221 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8281 aaaaggatct caagaagatc ctttgatctc tcgagtaaaa aaaatcctta gctttcgcta
     8341 aggatctgca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9181 ataagggcga cacggaaatg ttgaatactc atgcttaatt tctcctcttt aatagatctt
     9241 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9301 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
     9361 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
     9421 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
     9481 catgagctgt cctcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc
     9541 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
     9601 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
     9661 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
     9721 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
     9781 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ctcatgggag
     9841 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
     9901 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
     9961 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
    10021 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
    10081 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
    10141 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
    10201 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
    10261 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
    10321 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
    10381 ttgcaccatt cgatggtgtc
//
