LOCUS       sequence-358733-        6764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6764)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ANO10 (TMEM16KA-c001)
FEATURES             Location/Qualifiers
     source          1..6764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  60..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    254..388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(278..297)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     332..351
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(546..569)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(570..599)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(600..620)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        complement(2666..2757)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(2724..2741)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(3109..3114,3115..3131,3132..3137)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3307..3840
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  3907..4131
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(4265..4282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4436..5024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4516..4535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5195..5986,5987..6055))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5818..5837
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6056..6160)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6186..6640)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6323..6344)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6534..6553
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtcacatgga gttggcagga tgtttgatta aaaacataac aggaagaaaa atgccccgct
       61 gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt aaggattatt
      121 tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta atacgaaaat
      181 taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttcggat ctcctaggct
      241 caagcagtga tcagatccag acatgataag atacattgat gagtttggac aaaccacaac
      301 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      361 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      421 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      481 ggctgattat gatcctctag tacttctcga caagcttgtc gacggagctc gaattcggat
      541 cctcacttat cgtcgtcatc cttgtaatca tggtggtgat ggtgatggtg atgatggtgc
      601 gattggaagt agaggttctc tgcggttgcc ttctccttcc cgctttccat tggttcctcc
      661 ttcaggttct cggtcacgag cttcatttgc tgctgcttga gtgcctccaa agactcaaat
      721 tccagtctgg ctagtttcat ctggatatgc cgtggcttat caggtatggc aaatgcaagt
      781 ataaacttta aagccaggag tgcgtgctcc actgctacta caatcaaaat gaggtctgct
      841 tttgattctg gaaagactgc attcacttgt ggtgacattc caatcagcgc acagttagtg
      901 accacagata taacactcat cgtttcaaaa gccaactgcc acacaccaat attggctgaa
      961 ggttctgaga atggacgttt gaagaccctg cacattttta aggcatctga atttacttca
     1021 gtgaagttat ttaacacagc aaaggcagct gctaatgggt aaacacagga gaaaaggctc
     1081 acataaccaa actgcaggaa taactccaag taatcatcaa aggtgcccaa ataagttccc
     1141 atttcttttt ccaggatgac ttgttcatat aatgtagcat caatgtctgc ctttaaagcc
     1201 tgcaccttcc tcttcacccg cacaccatgc ttcctttgga gccaataagg aagaaaagat
     1261 tccataattt ggttgaggat ctgggaggta attaggagag tggccaagct ctggcgcaaa
     1321 agcttcatat ctttcaagac aaaggcaata tagaagagtg aggcaaagca attgaggaag
     1381 ttgaacacta aaactttcag aattagatgg ttctgatagg cagattccaa tctgtgattc
     1441 tcccatgaag ttaaaaactc ggcagcatat cgatagagac gattcatgat ctcaatcaca
     1501 atggcataga tgatgctggg cacatacaac aggacactgg tccactcaga cccgctgttc
     1561 tcatgtagac ccaaggccca aacctccatg tcgaagtaaa tcatcatgac atacagtgag
     1621 aaatagaggc agaggcacac gaatggcagg gagaccaggt aaatgcgcaa ctgtctcttg
     1681 tagctggggt acagaggctc ctccttccca gtgatggaat tgatacccaa gacaccatga
     1741 aatcctggcc ggggctcctc aaactttctc ttcatgagca gtgtccccca cctgtaggtc
     1801 atgttggcac agccacgctt ccacagttcc agaatcaccg tggaccagat gaggttgaac
     1861 gaggcaaaga tcacgtactt gtcatagtct tcccacacaa acaagtagta aggtaaccca
     1921 atgacagcca tggggattaa tgcaaaagtg aaatactcca aaaatccaaa gtacagagca
     1981 attgtttccc caaagtagcc acgaatactg tctatgggct gatacttcaa agcaaaccga
     2041 gtgtaccagg tgtcctcaag cttcttcagg gcttcactgt catgcagtgg aaacacctga
     2101 atcacgatgc cagacgtgag caatcttctc aacaatgatt ttcctggata caactttgcc
     2161 tgagggtaac cagggatcat tttttcatct ttagctctaa gattttcaag ttcatgtttg
     2221 ataatgaatt gacattctgc cattgtcagg aaatcatcat tgttatcatc aaaacctttg
     2281 aagttctgtc tggttctgta tgtgaaggct ctcatggtgt tatcattgca ctcttttacc
     2341 aatcccactg cttctgcccc tagtaacatt ctaatcttgg aggcaccaac aagatataag
     2401 ttctgatttt ctagtgtttc ttgttcatat ttatttaaca atggtctaaa caacaactgg
     2461 gcacctccat cttttttttt agctataatt ctgtttttca gccattcttt ggtttcttct
     2521 ttgacatcct gagcaagttc tatgaccacc aaaggtgtga aagaactctc agaagtatcc
     2581 aaagctgata acgtcacttt catagtatat ctccttctta agtagatccg cgcccgatgg
     2641 tgggacggta tgaataatcc ggaatattta taggtttttt tattacaaaa ctgttacgaa
     2701 aacagtaaaa tacttattta tttgcgagat ggttatcatt ttaattatct ccatgatcta
     2761 ttaatattcc ggagtatacg tagccaacca ctagaactat agctagagtc ctgggcgaac
     2821 aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc
     2881 accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg
     2941 cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc
     3001 gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag
     3061 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtt gacataagcc
     3121 tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     3181 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     3241 ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     3301 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     3361 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
     3421 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc cacctactcc
     3481 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
     3541 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccaag
     3601 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg cgagcaccgg
     3661 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
     3721 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
     3781 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
     3841 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attgtcacaa
     3901 cgccgcgaat atagtcttta ccatgccctt ggccacgccc ctctttaata cgacgggcaa
     3961 tttgcacttc agaaaatgaa gagtttgctt tagccataac aaaagtccag tatgcttttt
     4021 cacagcataa ctggactgat ttcagtttac aactattctg tctagtttaa gactttattg
     4081 tcatagttta gatctatttt gttcagttta agactttatt gtccgcccac acccgcttac
     4141 gcagggcatc catttattac tcaaccgtaa ccgattttgc caggttacgc ggctggtcta
     4201 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     4261 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4321 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4381 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4441 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4501 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4561 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4621 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4681 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4741 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4801 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4861 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4921 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4981 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5041 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5101 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5161 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5221 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5281 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5341 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5401 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5461 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5521 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5581 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5641 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5701 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5761 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5821 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5881 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5941 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6001 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6061 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6121 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6181 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6241 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6301 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa gaacgtggac
     6361 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     6421 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     6481 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     6541 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     6601 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     6661 caaataagcg ttgatattca gtcaattaca aacattaata acgaagagat gacagaaaaa
     6721 ttttcattct gtgacagaga aaaagtagcc gaagatgacg gttt
//
