LOCUS       sequence-358736-       10517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CcCDP monocistronically and CuCBP and BaSP 
            bicistronically.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Schwaiger KN, Voit A, Wiltschi B, Nidetzky B
  TITLE     Engineering cascade biocatalysis in whole cells for bottom-up 
            synthesis of cello-oligosaccharides: flux control over three 
            enzymatic steps enables soluble production.
  JOURNAL   Microb Cell Fact. 2022 Apr 9;21(1):61. doi: 
            10.1186/s12934-022-01781-w.
  PUBMED    35397553
REFERENCE   2  (bases 1 to 10517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-04-9"; volume: "21(1):61. doi"; pages: " 
            10.1186/s12934-022-01781-w"
COMMENT     Sequence Label: pDUBI
FEATURES             Location/Qualifiers
     source          1..10517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..40
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             56..76
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             96..113
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             2595..2606
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2622..2645
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      complement(4173..4220)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4227..4321
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(7325..7342)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             7361..7372
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    7388..7404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7412..7418,7419..7435,7436..7441,
                     7442..7456))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    7420..7436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    7483..7623
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7538..7557
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7638..7655)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7809..8397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7889..7908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8456..9247,9248..9316))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9079..9098
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     RBS             9323..9334
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(9344..10426)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10388..10407
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10427..10504)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     join(10514..10517,1..16)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(10514..10517,1..15)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taatgttgtt
       61 taactttaag aaggagatat catatgggca gcagccatca ccatcaccat cacgggtcga
      121 cccgctatgg tcattttgac gatgaagcac gcgagtacgt tatcaccaca ccgcatactc
      181 cgtacccttg gatcaactat ctgggttcgg aacagttctt cagtcttttg tcgcaccagg
      241 ctggcggcta ttcgttttat cgcgatgcca agatgcgtag attgacaaga tacagataca
      301 acaacatacc agcagacgcg ggaggccggt atctttatgt aaacgacggt ggggacgttt
      361 ggacgccatc gtggcttcct gttaaagcag acttagatca ttttgaagcc agacatggtc
      421 ttggctacag taccattaca ggtgaaagaa atggggtacg ggttgagacc ttgtttttcg
      481 tcccagtcgg tgagaacgcg gaggtccaga aagttacggt cactaatacc tctgactcgt
      541 ataaatccct gacgttgttt tcttttgtcg aattttgtct ttggaacgcg caagacgatc
      601 aaactaacta ccagcgtaat ctgagcatag gagaggttga agtggaacaa gaatctccgc
      661 atggcagtgc aatttatcac cgtacagagt accgggagag acgcgatcac tacgcggtat
      721 tcgccgttaa tacgcaggcc gagggtttcg atacagatcg cgacacattt gtgggagcct
      781 ataactcgct tggtgaagcc gcagttcccc ttaagggaga aagtgcaaat agcgttgcaa
      841 gtggatggta tcctattggc tctcatagcg tggcagtgtc tttagcgcct ggagaatcca
      901 gagaattggt ttatgtgctt gggtacgtgg aaaatcctga tgaggagaag tgggcagacg
      961 acgcaaaaca ggtcgtgaat aaagaaagag ctcacgcttt attatcaaga tttgctacgt
     1021 ccgaacagac tgacgcagct ttcgccgctt tgaaggatta ttggacggat ttgttatcga
     1081 cgtattccgt tagttccaat gacgaaaaat tggaccggat ggttaatata tggaatcagt
     1141 accaatgcat ggtaaccttt aacatgagtc gctcggcgag ctttttcgag accggtattg
     1201 ggagagggat ggggtttcgt gatagcaacc aagaccttct gggctttgtt cacttaatcc
     1261 cagagcgggc gcgtgagcgc atcattgaca tagcgtcaac ccagttcgcg gacggttccg
     1321 cttatcacca ataccaacct ttaacgaagc gcggtaacaa cgacattggc tctgggttta
     1381 acgacgatcc tctttggtta atcgcgggaa ctgccgccta catcaaagaa actggtgact
     1441 tctccatatt agatgagccc gtaccttttg ataacgaacc ggggtcagaa gttccgttat
     1501 ttgagcacct gacccgtagt tttgagttta ccgtaaccca tcggggtccc cacggtttgc
     1561 ctcttattgg ccgcgcagat tggaacgatt gtttgaatct taattgcttc agtacaaccc
     1621 cgggcgagtc atttcagaca acggagaacc aagccggtgg cgtagcggag tcaacgttca
     1681 tagccgcgca attcgtttta tatggggagc agtatgccga gttggcagcg cggcgcgggt
     1741 tggccgacgt agccgataga gcaagagggc atgtggctga aatgagagac gcacttctga
     1801 ccgacgggtg ggacggcagc tggttcttga gagcttatga ctattatgga aaccctatcg
     1861 gcaccgacgc acatgacgaa gggaagattt ggatcgaacc gcaagggttc gcggtgatgg
     1921 ctggagtcgg tgtaggcgaa gggcctcaag atacggacgc tcccgctatt aaggcgcttg
     1981 attctgtgaa cgaaatgttg gcaaccgatc acggtatggt ccttcaatac ccagcataca
     2041 ctacatacca agtacacatg ggtgaagtat ccacttatcc acccgggtat aaagagaatg
     2101 gaggtatatt ttgccacaat aatccttggg ttatcatagc ggaaactgtc gtcggaagag
     2161 gcggacgggc atttgattac tacaagagaa ttactcccgc gtatcgcgag gacataagcg
     2221 acgttcaccg tcttgaaccc tatgtctatg cacaaatgat agcagggaag gaggcggtcc
     2281 gtcacggtga agcaaagaac agctggctta ccgggactgc ggcttggaac tttgtgaccg
     2341 tgtcacagta ccttcttggg gttcgcccag agtacgacgg cttggtggta gacccgcaga
     2401 tcggacctga cgtacctagc tttacggtta cgcgtgtcgc ccgtggagcc acttacgaga
     2461 taacagtcac taacagtggg accgacggat cacgcggtcg ccttgtcgtt gatggaacac
     2521 ctgtagaagg caacttggtt ccttatgccc ccgcagggtc aacagttcgt gtcgatgtga
     2581 cattgtagtg tacaaaagag gagaaaaccc ccatggcaag ctggagccac ccgcagttcg
     2641 aaaagggtac catgaaaaat aaagtccaac tgattaccta tgcggatcgt ctgggtgatg
     2701 gcaccattaa aagcatgacg gacatcctgc gtacccgctt tgatggcgtt tatgacggtg
     2761 tccacattct gccgtttttc accccgtttg atggcgccga cgcaggtttc gatccgatcg
     2821 accataccaa agttgatgaa cgtctgggta gctgggatga cgtcgccgaa ctgtctaaaa
     2881 cccacaacat tatggtggat gcaatcgtta atcacatgtc atgggaatcg aaacagttcc
     2941 aagatgttct ggccaaaggc gaagaatcag aatattaccc gatgtttctg accatgagca
     3001 gcgtgttccc gaacggtgcc acggaagaag acctggcagg catttatcgt ccgcgtccgg
     3061 gtctgccgtt tacccactac aaattcgcag gcaaaacgcg tctggtctgg gtgtctttta
     3121 ccccgcagca agtggacatc gatacggaca gtgataaagg ttgggaatat ctgatgtcca
     3181 ttttcgacca aatggcggcc agccatgtgt cttatatccg cctggatgcg gttggctacg
     3241 gtgccaaaga agcaggcacc agttgcttta tgaccccgaa aacgttcaaa ctgatttccc
     3301 gtctgcgcga agaaggcgtg aaacgcggtc tggaaattct gatcgaagtc cacagctatt
     3361 acaaaaaaca ggtggaaatc gcgtctaaag tggatcgcgt ttatgacttt gccctgccgc
     3421 cgctgctgct gcatgccctg agtaccggcc acgtcgaacc ggtggcccat tggacggata
     3481 ttcgtccgaa caatgctgtt accgtcctgg atacgcatga cggcatcggc gttattgata
     3541 tcggttcaga tcagctggac cgctcgctga aaggtctggt gccggacgaa gatgtggata
     3601 acctggtgaa tacgattcat gcgaacaccc acggcgaatc acaggcagct accggtgcgg
     3661 cggcatcgaa cctggatctg taccaagtta atagtaccta ctactccgct ctgggctgta
     3721 acgatcagca ctacatcgct gcgcgtgcgg tgcagttttt cctgccgggt gttccgcaag
     3781 tctattacgt gggcgccctg gcaggtaaaa atgatatgga actgctgcgc aaaaccaaca
     3841 atggtcgtga cattaaccgc cattattaca gcacggccga aatcgatgaa aatctgaaac
     3901 gtccggtggt taaagcactg aacgctctgg cgaaatttcg caatgaactg gatgcttttg
     3961 acggcacctt ctcatatacc acggatgacg atacgagtat ttcctttacc tggcgtggtg
     4021 aaacgtcgca ggccaccctg acgttcgaac cgaaacgcgg cctgggtgtt gataatacca
     4081 cgccggtcgc aatgctggaa tgggaagaca gtgctggtga ccatcgctcc gacgacctga
     4141 tcgctaatcc gccggttgtt gcgtaaaagc ttcaaaaaac ccctcaagac ccgtttagag
     4201 gccccaaggg gttatgctag gagctcgact cctgttgata gatccagtaa tgacctcaga
     4261 actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa
     4321 tggatcctaa aaaaaatcct tagctttcgc taaggatgct agcttacttg aactccaggg
     4381 tcacgctatg ctctttacca tcggtgaaca tcgggataat gcagttgtcg gttttcttac
     4441 cgtcaatacg cagatcgtac gcgctgtcga tcagacgcgc aaagccacgc ggtttggtaa
     4501 tggtgatgtt atagctgcaa cccggcgcct tcaggctgta ttccagacgg gtttcgtcct
     4561 cacgcagaac cgggctgaac ataatgccat ccttcacgta ctcaataccc gccaggctgg
     4621 tgatggtcag gttcagccag gtcgcggtac cgctcagcag cggaccaatg ttctcgccgc
     4681 tgtccgcgtt aatatactgg gtgcagatac gcgggttacc cgccagtttg aacgggtctt
     4741 ccaggttctt gtacggcagc accagatcga tcatatagta cgccagacgc gccatctctt
     4801 tcgccagcgc tttatcctta acgcttttcg ccgccttcgc cagcgccgcg gtcgccatca
     4861 tgctcgcatg cttaaacacc gcgccgtttt cacggtcacc atagaagtag tgatcggtcg
     4921 cggtgtcgtt cgccagtttg ttcagatccg ccggggtggt cagacggatg ccatacgggg
     4981 tcagcatgta tttcttcagg gtgtcaacca taatgccgat ctgctcatcg gtcgccacac
     5041 cgctcagaat cgcccagcta aagctgttca ggaaataggt accatcgatg ttcgggtccg
     5101 cgctcagttt gtcgcccttc gcaccaacat aggtgaagct gccatctttg taacggttca
     5161 gcagcacacg cgcaaagaag ttacccttcc acgcgtattt ttgaatcttt tcgctcagcg
     5221 cgctgctgat tttacgcatg ctctgttcca gctccgcgtc cttatccagt tccgcgatct
     5281 ccgccaggtc atcaaccgcc accttcagca gaaacgcgtt cataacgctc tcgctatagt
     5341 cgctcatgaa acgatcgccg tatttgccac cggtttcttt cagttgctta tagtacagtt
     5401 tctccttctg cgcaccgtcc acatagtcgt tatcaatctt caggcaatcg ttccagtccg
     5461 ccagatccag cagcggcaga ccatggttac caacgctgat gaacgcgctg tactgaatga
     5521 tcgctttcag ggtgtcacgc accgcacgct tttcgttgtt accatccgca accggcactt
     5581 cctcgttcag gatgctggta tccttggtat aaatgatgta acgatagtac gcttgcagca
     5641 gccacaggct gtcatcgcta tacaggcccg gctgtttacc aacccagtag aagttgtggt
     5701 tcgcatagcc cattttgtaa acgttgctgg tccactcaaa cagcaggttc ttcacgaagt
     5761 cttggtaacc caggttgatg aagtagtaca tgctcgcgta cagatcctga atttcacgga
     5821 agccgatctc acgataaccc ttttgggtct ggcaaaagct acggctcatg aaggtttggt
     5881 acaggatctg aaacgccagg tttttgttaa aacccgcgtt gaacatcttg tcttcggtct
     5941 tgaactcgat gtacttgcag tacttttctt ggaagtcgat aacctcacgc agcgcgctct
     6001 gcacgctatc gctcgcttcg aaataacgca gcagcgcttc cagctctttc ggcatggtgg
     6061 tgtcatcgta gttcgggtta tccttgctgc tgctcatacc ggtaaaggta tcaatctcga
     6121 tgtcttggcc cggcataatg gtgaacgggg tacccagcgc aaagaagccc ggacctttac
     6181 ggttcagctt gttgttcagg tgttcgccac ccgccgggtg ctccagggta ccgttaccca
     6241 caaagtcgtt ataatcggtg cagaagctct tcgggaagat acgtttaccg tccttacgaa
     6301 caaacatggt cacgaaacgg gtgtcttcgt tgtatttctt acgatagtaa tccggggtaa
     6361 tgccgatgaa ctcgccgttg tcgttataca gcgccgcgct ctgcataata acgttggtgt
     6421 aggtcacatc ttcaaagatc gcgtgggtcg ccgcggtacc aaacatgccg gtgtaggtga
     6481 tgctcaggtt acgtttacgt tcgctgttgt tcttgatgtt gatgatttgg atctcggtcg
     6541 ccagcggcag acctttcttc tgcgggcaca gaaaaatggt acgggtgatc tccagcttgc
     6601 acgcggtttt atacttaata acggtacggt tgcagctgtg aatgcatttc gcttccagga
     6661 tgttgtcatc gtccacgctg ccgctgtaga agatttgctt gccgttctca tacaggtaaa
     6721 actgacggtt cgccgggaag ccgttttcct cttgacgcat gtcgtaacgg gtcgccagaa
     6781 cctgggtcgc cgcatggcta cgaaagctgc cacgaccgaa acgatcaacc acgctcttcg
     6841 gggtggtttg cagggtacgg ttaaagccca gacggttacc aatcagcagg ttaacgtaga
     6901 agtgcggacc cgggctcggt ttcttcaggt cgatcacgtg ttcgccgttc tcggtcaggg
     6961 tacccgccca gtttttaaca tcgctcacgg tgctctgaat gatatcgtcc agctcttgcg
     7021 gcagtttaac cttctggccg tcttcaaaca ccaggcaata acgcataccc tccagcgcca
     7081 ggcaaacgct tttgttttgc acgtattcgt tcaggaacgc cgccagcttg ctgttctcca
     7141 gcagaacctg aatcaccggc gctttgatcg gcaggccggt aataccgatc acgttcttgc
     7201 agctgctgtc gctgtagatc gcatccgcgc agccgctcat cgcaataatg ctaccgaatt
     7261 tttgtttcag aatctcgaac ggggtcagtt tgcagctacg cggggtcact ttggtaatgg
     7321 agccgtgatg gtgatggtga tgcgatcctc tcatactagt tttctcctct ttagattctg
     7381 tgtgaaattg ttatccgctc acaattgaat ctattataat tgttatccgc tcacaaagca
     7441 aataaatttt ttatgatttg gatccgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     7501 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     7561 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7621 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7681 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7741 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7801 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7861 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7921 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7981 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8041 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8101 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8161 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8221 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8281 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8341 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8401 gatcctttga tctctcgagt aaaaaaaatc cttagctttc gctaaggatc tgcagttacc
     8461 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8521 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8581 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8641 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8701 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8761 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8821 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8881 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8941 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9001 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9061 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9121 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9181 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9241 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9301 aatgttgaat actcatgctt aatttctcct ctttaataga tcttcactgc ccgctttcca
     9361 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9421 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     9481 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     9541 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcctcgg
     9601 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
     9661 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     9721 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     9781 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     9841 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     9901 cgaccagatg ctccacgccc agtcgcgtac cgtcctcatg ggagaaaata atactgttga
     9961 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
    10021 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
    10081 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
    10141 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
    10201 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
    10261 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
    10321 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
    10381 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
    10441 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg
    10501 tgtcgaattc agttaat
//
