LOCUS       sequence-358743-       10244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid supports expression of 2 gRNAs targeting D.suzukii bTub, 
            Opie-mVenus tagged, and can be integrated with pBac..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Kandul NP, Liu J, Buchman A, Shriner IC, Corder RM, Warsinger-Pepe 
            N, Yang T, Yadav AK, Scott MJ, Marshall JM, Akbari OS
  TITLE     Precision Guided Sterile Males Suppress Populations of an Invasive 
            Crop Pest
  JOURNAL   GEN Biotechnology (2022) 1:4, 372-385
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GEN 
            Biotechnology (2022) 1:4, 372-385"
COMMENT     Sequence Label: 1056D
FEATURES             Location/Qualifiers
     source          1..10244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        5071..5490
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        5511..5586
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(6375..6377,6378..6380,6381..7094)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6420..6441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6681..6700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7028..7049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7213..7334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7250..7269)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7304..7323
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7347..7766
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        7787..7862
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     repeat_region   9722..9756
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9937..9953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9937..9953)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9950..9972)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9961..9977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9985..9991,9992..10009,10010..10015))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10030..10051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10168..10185)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg
     4081 gtttaaacac ccgtacttta agttatcctg atttggaaaa aaattttaac atttgaagtt
     4141 tttattgttt agaaaattca atggagtcgc cccacatatg ggacagggtt ggctttatct
     4201 ggtgcctgtc agtgtgttta atacttttcc atctattact gtcaagtata aactaaagtt
     4261 tatatagacc gttttcccat tttcataaac aaatttggaa gactgcaaaa tcttattttg
     4321 cttctccaag tttgtatgct cctttattaa gcattccttt gtttccttta tgaatttcaa
     4381 ctttagagac ctgcagaacc tgatagattg tggaaatcaa ttgttccata gtatatttcc
     4441 gctagaatca atcattcgta aaggagtttc tgctgtggcg aatatactgc catcgacatc
     4501 acacttctgt ggacctgcaa atccctgttt gtagttcgaa tgacctgagc ttccatcgta
     4561 tccataacta agtagaagtt cggcgattaa aaatgttgtc ccttgatctt tccttatttt
     4621 ctaaacaact tttttttgta aagttaaaat ccggccagct gtgtggttaa gcaaacttag
     4681 caaaggcact tctgcttttg tgtcattcgt tctttttgtt tgcaacttcg tagtacgagg
     4741 aaaaaggttg tttttcgttt gatctctaat tgagatattt tgttgcttgg ttaaattttg
     4801 atcttaaaga acagctagtg cgtcgttaaa tagtattttc tccaatttcg cgaggggctg
     4861 acttattaag gtgtgcatgc tcttggcttt ttcagggcag atagcaaaca gttttataat
     4921 acatgccaga tctgatttac tgcccttacg cgcagccgca ctagctgaat ttattaataa
     4981 tttaatattt ccagccgtgg atttgatcaa attttaagct gctcttctct gagcccttgc
     5041 tctcgacaac aaataaggca atgcggccgc tttttgctca cctgtgattg ctcctactca
     5101 aatacaaaaa catcaaattt tctgtcaata aagcatattt atttatattt attttacagg
     5161 aaagaattcc ttttaaagtg tattttaacc tataatgaaa aacgattaaa aaaaatacat
     5221 aaaataattc gaaaattttt gaatagccca ggttgataaa aattcatttc atacgtttta
     5281 taacttatgc ccctaagtat tttttgacca tagtgtttca attctacatt aattttacag
     5341 agtagaatga aacgccacct actcagccaa gaggcgaaaa ggttagctcg ccaagcagag
     5401 agggcgccag tgctcactac tttttataat tctcaacttc tttttccaga ctcagttcgt
     5461 atatatagac ctattttcaa tttaacgtcg cttgagtgtg caccagctgg gttttagagc
     5521 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     5581 cggtgctttt ttgcctacct ggagcctgag agttgttcaa taaaataaaa atgtttcgtt
     5641 tttttgcttt cgccagtatt tattattttt catcaatatg tattcaattt ggtatgtatt
     5701 tagtaattgt aatatataga caatggtttt ccgttgacgt acatacatct gacgtgtgtt
     5761 tatttagaca taatagttat gttttcacat ctttttaatg ttcgcttaat gcgtatgcat
     5821 taattaacat actcggtggc ctccccacca ccaacttttt tgcactgcaa aaaaacacgc
     5881 ttttgcacgc gggcccatac atagtacaaa ctctacgttt cgtagactat tttacataaa
     5941 tagtctacac cgttgtatac gctccaaata cactaccaca cattgaacct ttttgcagtg
     6001 caaaaaagta cgtgtcggca gtcacgtagg ccggccttat cgggtcgcgt cctgtcacgt
     6061 acgaatcaca ttatcggacc ggacgagtgt tgtcttatcg tgacaggacg ccagcttcct
     6121 gtgttgctaa ccgcagccgg acgcaactcc ttatcggaac aggacgcgcc tccatatcag
     6181 ccgcgcgtta tctcatgcgc gtgaccggac acgaggcgcc cgtcccgctt atcgcgccta
     6241 taaatacagc ccgcaacgat ctggtaaaca cagttgaaca gcatctgtta cagcgacaca
     6301 acatgagccg gtccaacaac gccaacgcgc ccacgccatc caaccgccgc cgcaacctgt
     6361 ctctggtggc tagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     6481 gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg
     6541 tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc gcccgctacc
     6601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     6721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     6781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg
     6841 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     6901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6961 tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc aacgagaagc
     7021 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     7081 agctgtacaa gtaagcgctg actctagatc ataatcagcc ataccacatt tgtagaggtt
     7141 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7201 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7261 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7321 atcaatgtat cttaaagctt cttaagtttt tgctcacctg tgattgctcc tactcaaata
     7381 caaaaacatc aaattttctg tcaataaagc atatttattt atatttattt tacaggaaag
     7441 aattcctttt aaagtgtatt ttaacctata atgaaaaacg attaaaaaaa atacataaaa
     7501 taattcgaaa atttttgaat agcccaggtt gataaaaatt catttcatac gttttataac
     7561 ttatgcccct aagtattttt tgaccatagt gtttcaattc tacattaatt ttacagagta
     7621 gaatgaaacg ccacctactc agccaagagg cgaaaaggtt agctcgccaa gcagagaggg
     7681 cgccagtgct cactactttt tataattctc aacttctttt tccagactca gttcgtatat
     7741 atagacctat tttcaattta acgtcgtgtt ctggatgtgg tgcgaagttt tagagctaga
     7801 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     7861 gcttttttgc ctacctggag cctgagagtt gttcaataaa ataaaaatgt ttcgtttttt
     7921 tgctttcgcc agtatttatt atttttcatc aatatgtatt caatttggta tgtatttagt
     7981 aattgtaata tatagacaat ggttttccgt tgacgtacat acatctgacg tgtgtttatt
     8041 tagacataat agttatgttt tcacatcttt ttaatgttcg cttaatgcgt atgcatctcg
     8101 agcctatcta tgcaaatctc agtgtgggat gtaagccaag ttgagtactt tttcttgaaa
     8161 tgattaaaat ttcttttgca atatgtctat ttgcgggaca atctataaca aaaagtttcg
     8221 ctttgttttt ttaaaacatc ctcataagca tctgaaacag ctattgaagc tctaaatctg
     8281 tcaaatattg cctgctgttt ctcacgcttt ttctctccag aactccaagt ctcaaacaat
     8341 tctgaattgg ccaacctgag gttgtgacct aaaaatgtat attttagtgt tagtttttat
     8401 tgttaatttg tacacgtaaa gcggcaatta aaagcactca acacaaacaa atccaacttg
     8461 tgacgaccac aaacatatag tacgaaccgc tctctttcga tgccatgaca acttgtttga
     8521 tataaactta gattggtgct ttatttttaa taattttaaa cggactcttt tttccgcaga
     8581 gtagctttcg tgcacccgtg ataccgaaca gctgttttaa cagcctatat attttaacag
     8641 atcgtgactc agataaaatt tctgttacgt aacattgaag tttgatttta atattaacga
     8701 ctttcaaaaa atggattttt gcccacaaaa cgtgatcagc cagcctaaag tgcactcgct
     8761 gcaaatagga ccgcacgcgt aatgtatttt agtattttct acgagttaaa ttaatttttg
     8821 tatttgacgt ttcgggatgt ttttaaaaaa ggtagttgtt gttgcggcag tacccagtag
     8881 ccttccagcg gctcgaaact gaacatttga tctcatcctg aaaagaaata ttgctatgct
     8941 catttctaag aaaatgacca acctccatct tgtgtcctgc ttggcaagac tttgcacctg
     9001 ttgcaccaaa agtacattat tttcactacg cttgcttgaa tagaattctc atcggcgcgc
     9061 ctaggccggc cgatctcgga tctgacaatg ttcagtgcag agactcggct acgcctcgtg
     9121 gactttgaag ttgaccaaca atgtttattc ttacctctaa tagtcctctg tggcaaggtc
     9181 aagattctgt tagaagccaa tgaagaacct ggttgttcaa taacattttg ttcgtctaat
     9241 atttcactac cgcttgacgt tggctgcact tcatgtacct catctataaa cgcttcttct
     9301 gtatcgctct ggacgtcatc ttcacttacg tgatctgata tttcactgtc agaatcctca
     9361 ccaacaagct cgtcatcgct ttgcagaaga gcagagagga tatgctcatc gtctaaagaa
     9421 ctacccattt tattatatat tagtcacgat atctataaca agaaaatata tatataataa
     9481 gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa
     9541 agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc
     9601 agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg
     9661 tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat
     9721 gcatgcgtca attttacgca gactatcttt ctagggttaa aaaagatttg cgctttactc
     9781 gacctaaact ttaaacacgt catagaatct tcgtttgaca aaaaccacat tgtggccaag
     9841 ctgtgtgacg cgacgcgcgc taaagaatgg caaaccaagt cgcgcgagcg tcgactctag
     9901 aggatccccg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     9961 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10021 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10081 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10141 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10201 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggt
//
