LOCUS       sequence-358818-        1942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing L3S2P21 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_039
FEATURES             Location/Qualifiers
     source          1..1942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     480..499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(718..822)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      925..1513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1414..1433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1799..1942,1..648,649..717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
       61 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
      121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
      181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
      241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
      301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
      361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
      421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
      481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
      541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
      601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
      661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
      721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
      781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
      841 gccacctgtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      901 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      961 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1021 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1081 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1141 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1201 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1261 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1321 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1381 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1441 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1501 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1561 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtagtcggtc
     1621 tcattcttaa ctcggtacca aattccagaa aagaggcctc ccgaaagggg ggcctttttt
     1681 cgttttggtc caacgtgaga ccaagattat caaaaaggat cttcacctag atccttttaa
     1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1921 gtgctgcaat gataccgcgg ga
//
