LOCUS       sequence-358846-        7881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express SpCas9-mSA*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Liang SQ, Liu P, Smith JL, Mintzer E, Maitland S, Dong X, Yang Q, 
            Lee J, Haynes CM, Zhu LJ, Watts JK, Sontheimer EJ, Wolfe SA, Xue W
  TITLE     Genome-wide detection of CRISPR editing in vivo using GUIDE-tag.
  JOURNAL   Nat Commun. 2022 Jan 21;13(1):437. doi: 10.1038/s41467-022-28135-9.
  PUBMED    35064134
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-01-21"; volume: "13(1):437. doi"; pages: " 
            10.1038/s41467-022-28135-9"
COMMENT     Sequence Label: SpCas9-mSA*
FEATURES             Location/Qualifiers
     source          1..7881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          813..945
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     975..994
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        975..993
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1014..1023
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1029..1055
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1092..1118
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1164..1190
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1191..5291
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1191..5291
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1191..5291
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5304..5324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5325..5414
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5427..5453
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             5496..5837
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     primer_bind     complement(5866..5882)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5866..5882)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5895..5921
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5964..5990
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     complement(6054..6073)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    6083..6164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6108..6127
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6362..6950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6442..6461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7031..7825)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7031..7825)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7142..7161)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7752..7771
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacaggggta ccgaagccgc
      961 tagcgctacc ggtctaatac gactcactat agggcgaatt gggtaccggg cccgccacca
     1021 tggctagccc cgccgccaag cgcgtgaagc tggacggcgg cagcggcggc ggcagcggcg
     1081 gcggcagcgg cccggcagct aagagggtga aactggatgg cggtagcggc ggaggcagcg
     1141 gtggcggcag cggccccctc gagccggcag ctaagagggt gaaactggat gacaagaagt
     1201 actccattgg gctcgatatc ggtaccaaca gcgtcggctg ggccgtcatt acggacgagt
     1261 acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga
     1321 agaacctcat tggagccctc ctgttcgact ccggggagac ggccgaagcc acgcggctca
     1381 aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga
     1441 tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct
     1501 ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg
     1561 aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag ctggtagaca
     1621 gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcacatg atcaaatttc
     1681 ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct
     1741 ttatccaact ggttcagact tacaatcagc ttttcgagga gaacccgatc aacgcatccg
     1801 gcgttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc
     1861 tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt atcgccctgt
     1921 cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat gccaagctgc
     1981 aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag atcggcgacc
     2041 agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg ctgagtgata
     2101 ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg atcaagcgct
     2161 atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag caactgcctg
     2221 agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg
     2281 gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa aaaatggacg
     2341 gcaccgagga gctgctggta aagctgaaca gagaagatct gttgcgcaaa cagcgcactt
     2401 tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct atcctcaggc
     2461 ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag aaaatcctca
     2521 catttcggat accctactat gtaggccccc tcgctcgggg aaattccaga ttcgcgtgga
     2581 tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc gtggataagg
     2641 gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat ctgcctaacg
     2701 aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat aacgagctca
     2761 ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct ggagagcaga
     2821 agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg aaacagctca
     2881 aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc ggagtggagg
     2941 atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt aaagacaagg
     3001 acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc acccttacgt
     3061 tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat ctcttcgacg
     3121 acaaagtcat gaaacagctc aagagacgcc gatatacagg atgggggcgg ctgtcaagaa
     3181 aactgatcaa tggcatccga gacaagcaga gtggaaagac aatcctggat tttcttaagt
     3241 ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct ctcaccttta
     3301 aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac gagcacatcg
     3361 ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg
     3421 atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc gagatggccc
     3481 gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg aagaggattg
     3541 aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt gaaaacaccc
     3601 agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac atgtacgtgg
     3661 atcaggaact ggacatcaac cggttgtccg actacgacgt ggatcatatc gtgccccaaa
     3721 gctttctcaa agatgattct attgataata aagtgttgac aagatccgat aaaaatagag
     3781 ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc
     3841 agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact aaggctgaac
     3901 gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt gttgagacac
     3961 gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc aagtacgatg
     4021 aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag ctggtctcag
     4081 atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac caccatgcgc
     4141 atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat cccaagctgg
     4201 aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg atcgcaaagt
     4261 ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat attatgaatt
     4321 ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca cttatcgaaa
     4381 caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg acagtccgca
     4441 aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag accggaggct
     4501 tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag
     4561 attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac agtgtactgg
     4621 ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg
     4681 gcatcacaat catggagcga tccagcttcg agaaaaaccc catcgacttt ctcgaagcga
     4741 aaggatataa agaggtcaaa aaagacctca tcattaagct gcccaagtac tctctctttg
     4801 agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg
     4861 agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac tatgaaaagc
     4921 tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa cacaaacact
     4981 accttgatga gatcatcgag caaataagcg agttctccaa aagagtgatc ctcgccgacg
     5041 ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc atcagggagc
     5101 aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct gcagccttca
     5161 agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag gtcctggacg
     5221 ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac ctctctcagc
     5281 tcggtggaga cggcaccggc gggcccaaga agaagaggaa ggtataccca tacgatgttc
     5341 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcgtat ccttatgacg
     5401 ttccagatta cgctggatcc gccgctccgg cagctaagaa aaagaaactg gatttcgaat
     5461 ccggagaatt cggggcccag ccggccagat ctatggcgga agcgggtatc accggcacgt
     5521 ggtacaacca gtctggttct accttcaccg ttaccgcggg tgcggacggt aacctgaccg
     5581 gtcagtacga aaaccgtgcg cagggcactg gttgccagaa ctctccgtac accctgaccg
     5641 gtcgttacaa cggtaccaaa ctggaatggc gtgttgaatg gaacaactct accgaaaact
     5701 gccactctcg taccgaatgg cgtggtcagt accagggtgg tgcggaagcg cgtatcaaca
     5761 cccagtggaa cctgacctac gaaggtggtt ctggtccggc gaccgaacag ggtcaggaca
     5821 ccttcaccaa agttaaaccg tctgcggcgt ctggatcccc cgggggatcc actagttcta
     5881 gaggatccgc cgctcccgcc gccaagcgcg tgaagctgga cggcggcagc ggcggcggca
     5941 gcggcggcgg cagcggctcc ggaccggcag ctaagagggt gaaactggat tgataagcgg
     6001 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat ctcgatgagt
     6061 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     6121 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     6181 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     6241 tctacaaatg tggtacttaa gagggggaga ccaaagggcg agacgttaag gcctcacgtg
     6301 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6361 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6421 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6481 gctctcctgt tccgaccctg ccgcttacgg gatacctgtc cgcctttctc ccttcgggaa
     6541 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6601 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6661 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6721 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6781 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6841 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6901 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6961 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7021 tcatgccgtc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     7081 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     7141 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     7201 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     7261 catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt
     7321 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     7381 ccatccgagt acgtgctctc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     7441 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     7501 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     7561 ttcccgcttc agtgacaacg tcgagtacag ctgcgcaagg aacgcccgtc gtggccagcc
     7621 acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga
     7681 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     7741 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     7801 cgtgcaatcc atcttgttca atcataatat tattgaagca tttatcaggg ttcgtctcgt
     7861 cccggtctcc tcccatgcat g
//
