LOCUS       sequence-35890-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Miller HB, Saunders KO, Tomaras GD, Garcia-Blanco MA
  TITLE     Tat-SF1 is not required for Tat transactivation but does regulate 
            the relative levels of unspliced and spliced HIV-1 RNAs.
  JOURNAL   PLoS One. 2009  . 4(5):e5710.
  PUBMED    19479034
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2009  . 4(5):e5710."
COMMENT     Sequence Label: pSuper-TatSF1 AB
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(105..121)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(106..122)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(146..165)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(147..165)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(175..192)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(175..191)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(184..206)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(470..491)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     682..701
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgggatct tataagttct gtatgagacc acagatcccc gagtcagatg acaaggaagt
       61 tcaagagact tccttgtcat ctgactcttt ttggaaaagc ttatcgatac cgtcgacctc
      121 gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
      181 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      241 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      301 ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa
      361 ttcgcgttaa atttttgtta aatcagctca tttttttaac caataggccg aaatcggcaa
      421 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
      481 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
      541 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
      601 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
      661 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
      721 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
//
