LOCUS       sequence-35891-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Miller HB, Saunders KO, Tomaras GD, Garcia-Blanco MA
  TITLE     Tat-SF1 is not required for Tat transactivation but does regulate 
            the relative levels of unspliced and spliced HIV-1 RNAs.
  JOURNAL   PLoS One. 2009  . 4(5):e5710.
  PUBMED    19479034
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2009  . 4(5):e5710."
COMMENT     Sequence Label: pSuper-TatSF1 GH
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(115..131)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(116..132)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(156..175)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(157..175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(185..202)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(185..201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(194..216)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(342..798)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(480..501)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     692..711
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttgggatctt ataagttctg tatgagacca cagatccccg agaatctgtg gaacttgcat
       61 taaagaagct tgtttaatgc aagttccaca gattctcttt ttggaaaagc ttatcgatac
      121 cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacgcgc
      181 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      241 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      301 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat
      361 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca tttttttaac caataggccg
      421 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
      481 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
      541 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
      601 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
      661 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
      721 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
      781 cgccgctaca gggcgcgtca
//
