LOCUS       sequence-358934-        3324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of  WT human  MOV10 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Arora R, Bodak M, Penouty L, Hackman C, Ciaudo C
  TITLE     Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts 
            retrotransposition.
  JOURNAL   EMBO Rep. 2022 Jul 20:e54458. doi: 10.15252/embr.202154458.
  PUBMED    35856394
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Jul 20:e54458. doi: 10.15252/embr.202154458."
COMMENT     Sequence Label: T10-T7-hMOV10
FEATURES             Location/Qualifiers
     source          1..3324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             182..214
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    2891..2902
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttaccatgg atctcccaga
      121 cgatcattac ctgtccaccc agacaatcct gagcaaagat cttaatgggg taccaggttc
      181 aatggctagc atgactggtg gacagcaaat gggtcccggg agcggcggtg agggctcagc
      241 cggcggagga ccggtcggag gcggatccat gcccagtaag ttcagctgcc ggcagctccg
      301 ggaggcgggc cagtgtttcg agagtttcct ggtcgttcgg ggactggaca tggagacaga
      361 tcgcgagcgg ctgcggacca tttataaccg cgacttcaag atcagctttg ggacccccgc
      421 ccctggcttc tcctccatgc tgtatggaat gaagattgca aatctggcct acgtcaccaa
      481 gactcgggtc aggttcttca gactcgaccg ctgggccgac gtgcggttcc cagaaaagag
      541 gagaatgaag ctggggtcag atatcagcaa acaccacaag tcactgctag ccaagatctt
      601 ttatgacagg gctgagtatc ttcatgggaa acatggtgtg gatgtggaag tccaggggcc
      661 ccatgaagcc cgagatgggc agctccttat ccgcctggat ttgaaccgca aagaggtgct
      721 gaccctgagg cttcggaatg gcggaaccca gtctgttacc ctcactcacc tcttcccact
      781 ctgccggaca ccccagtttg ctttctacaa tgaagaccag gagttgccct gtccactggg
      841 ccccggtgaa tgctatgaac tccatgtcca ttgtaagacc agctttgtgg gctacttccc
      901 agccacagtg ctctgggagc tgctgggacc tggggagtcg ggttcagaag gagccggcac
      961 attctacatt gcccgcttct tggctgccgt cgcccacagc cccctggctg cacagctgaa
     1021 gcccatgact cccttcaagc ggacccggat caccggaaac cctgtggtga ccaatcggat
     1081 agaggaagga gagagacctg accgcgctaa gggctatgac ctggagttaa gtatggcgct
     1141 ggggacatac tacccacctc cccgcctcag gcagctgctc cccatgcttc ttcagggaac
     1201 aagtatcttc actgccccta aggagatcgc agagatcaag gcccagctgg agacagccct
     1261 gaagtggagg aactatgagg tgaagctgcg gctgctgctg cacctggagg aactgcagat
     1321 ggagcatgat atccggcact atgacctgga gtcggtgccc atgacctggg accctgtgga
     1381 ccagaacccc aggctgctca cgctggaggt tcctggagtg actgagagcc gcccctcagt
     1441 gctacggggc gaccacctgt ttgccctttt gtcctcggag acacaccagg aggaccccat
     1501 cacatataag ggctttgtgc acaaggtgga attggaccgt gtcaagctga gcttttccat
     1561 gagcctcctg agccgctttg tggatgggct gaccttcaag gtgaacttta ccttcaaccg
     1621 ccagccgctg cgagtccagc accgtgccct ggagctgaca gggcgctggc tgctgtggcc
     1681 catgctcttt cctgtggcac ctcgggacgt cccgctgctg ccctcagatg tgaaactcaa
     1741 gctgtacgac cggagtctgg agtcaaaccc agagcagctg caggccatga ggcacattgt
     1801 tacgggcacc acccgtccag ccccctacat catctttggg cctccaggca ccggcaagac
     1861 tgtcacgtta gtggaggcaa ttaagcaggt ggtgaagcac ttgcccaaag cccacatctt
     1921 ggcctgcgct ccatccaact caggggctga cctactctgt caaaggctcc gggtccacct
     1981 tcctagctcc atctaccgcc tcctggcccc cagcagggac atccgcatgg tacctgagga
     2041 catcaagccc tgctgcaact gggacgcaaa gaagggggag tatgtatttc ccgccaagaa
     2101 gaagctgcag gaataccggg tcttaattac caccctcatc actgccggca ggttggtctc
     2161 ggcccagttt cccattgatc acttcacaca catcttcatc gatgaggctg gccactgcat
     2221 ggagcctgag agtctggtag ctatagcagg gctgatggaa gtaaaggaaa caggtgatcc
     2281 aggagggcag ctggtgctgg caggagaccc tcggcagctg gggcctgtgc tgcgttcccc
     2341 actgacccag aagcatggac tgggatactc actgctggag cggctgctca cctacaactc
     2401 cctgtacaag aagggccctg atggctatga cccccagttc ataaccaagc tgctccgcaa
     2461 ctacaggtct catcccacca tcctggacat tcctaaccag ctctattatg aaggggagct
     2521 gcaggcctgt gctgatgtcg tggatcgaga acgcttctgc cgctgggcgg gcctacctcg
     2581 acagggcttt cccatcatct ttcacggcgt aatgggcaaa gatgagcgtg aaggcaacag
     2641 cccatccttc ttcaaccctg aagaggctgc cacagtgact tcctacctga agctgctcct
     2701 ggccccctcc tccaagaagg gcaaagctcg cctgagccct cgaagtgtgg gcgtcatctc
     2761 cccgtaccgg aaacaggtgg agaaaatccg ttactgcatc accaaacttg acagggagct
     2821 tcgaggactg gatgacatca aggacttgaa ggtgggttca gtagaagaat tccaaggcca
     2881 agaacgaagc gtcatcctca tctccaccgt gcgaagcagc cagagctttg tgcagctgga
     2941 tctggacttt aatctgggtt tccttaagaa ccccaagagg ttcaatgtag ctgtgacccg
     3001 ggccaaggcc ctgctcatca tcgtggggaa cccccttctc ctgggccatg accctgactg
     3061 gaaagtattc ctggagttct gtaaagaaaa cggagggtat accgggtgtc ccttccctgc
     3121 caaactggac ctgcaacagg gacagaattt actgcaaggt ctgagcaagc tcagcccctc
     3181 tacctcaggg ccccacagcc atgactacct cccccaggag cgggagggtg aagggggcct
     3241 gtctctgcaa gtggagccag agtggaggaa tgagctctga gaattctgca gatatccagc
     3301 acagtggcgg ccgctcgagt ctag
//
