LOCUS       sequence-358938-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Deletion of BHLH domain of mouse TFAP4 in combination with 
            TFAP4_BHLH_3-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Schaefer M, Nabih A, Spies D, Hermes V, Bodak M, Wischnewski H, 
            Stalder P, Ngondo RP, Liechti LA, Sajic T, Aebersold R, Gatfield D, 
            Ciaudo C
  TITLE     Global and precise identification of functional miRNA targets in 
            mESCs by integrative analysis.
  JOURNAL   EMBO Rep. 2022 Jul 28:e54762. doi: 10.15252/embr.202254762.
  PUBMED    35899551
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Jul 28:e54762. doi: 10.15252/embr.202254762."
COMMENT     Sequence Label: TFAP4_BHLH_5-1
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     124..143
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        223..298
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    346..463
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        532..743
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      763..772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             772..795
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             802..822
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
       61 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      121 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      181 ttgtggaaag gacgaaacac cggggtcagt ggaatgttgg ctgttttaga gctagaaata
      241 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      301 ttttgaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
      361 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
      421 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
      481 cagcagagat ccactttggc gccggctcga gtggctccgg tgcccgtcag tgggcagagc
      541 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
      601 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      661 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      721 acgggtttgc cgccagaaca caggtgtcgt gacgcgggat ccgccaccat ggattacaaa
      781 gacgatgacg ataagatggc cccaaagaag aagcggaagg tcggtatcca cggagtccca
      841 gcagccgaca agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc
      901 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
      961 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
     1021 gaggccaccc ggctgaagag accgccagag aatatacacc gaacggaaga cccggatctg
     1081 ctatctgcaa gagatct
//
