LOCUS       sequence-358946-        1929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing L3S1P22 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_037
FEATURES             Location/Qualifiers
     source          1..1929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     146..165
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(384..488)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      591..1179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1080..1099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1452..1929,1..314,315..383))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
       61 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
      121 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
      181 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
      241 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
      301 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
      361 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
      421 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      481 gttccgcgca catttccccg aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag
      541 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      601 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      661 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      961 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1201 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1261 gattctgtgg ataaccgtag tcggtctcat tcttaagacg aacaataagg ccgcaaatcg
     1321 cggccttttt tattgataac aaaaaacgtg agaccaagat tatcaaaaag gatcttcacc
     1381 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1441 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1501 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1561 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
     1621 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1681 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1741 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1801 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1861 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1921 gtgttatca
//
