LOCUS       sequence-358948-       14133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary plant vector expressing eYGFPuv and GUS, both driven by a 35S
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14133)
  AUTHORS   Yuan G, Lu H, Tang D, Hassan MM, Li Y, Chen JG, Tuskan GA, Yang X
  TITLE     Expanding the application of a UV-visible reporter for transient 
            gene expression and stable transformation in plants.
  JOURNAL   Hortic Res. 2021 Nov 1;8(1):234. doi: 10.1038/s41438-021-00663-3.
  PUBMED    34719678
REFERENCE   2  (bases 1 to 14133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hortic 
            Res."; date: "2021-11-1"; volume: "8(1):234. doi"; pages: " 
            10.1038/s41438-021-00663-3"
COMMENT     Sequence Label: pAXY0002
FEATURES             Location/Qualifiers
     source          1..14133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2400..2648
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3338..3354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3338..3354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3351..3373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3362..3378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3386..3392,3393..3410,3411..3416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3431..3452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3643..4319
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4387..5184
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4444..4463)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5054..5073
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5224..5398
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5476..5500
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5925..6719
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6806..7394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7295..7314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7548..7565
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7580..7720
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7646..7665)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7806..7828
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8064..8258
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8324..9397)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9826..10455)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11756..11780
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11802..12054)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12086..12103)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12110..13927,14118..14132))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13928..14117
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatcttcat gctgcaacga acggaggtta
     1981 caccaacacc aggaaggaga tctatgaaga cggcggcatc ttggaggtca acttccgtta
     2041 cacttacgag ttcaacaaga tcatcggtga cgtcgagtgc attggacatg gattcccaag
     2101 tcagagtccg atcttcaagg acacgatcgt gaagagttgt cccacggtgg acctgatgtt
     2161 gccaatgtcc gggaacatca tcgccagctc ctacgcttac gccttccaac tgaaggacgg
     2221 ctctttctac acggcagaag tcaagaacaa catagacttc aagaatccaa tccacgagtc
     2281 cttctcgaag tcagggccca tgttcaccca cagacgtgtc gaggagactc tcaccaagga
     2341 gaaccttgcc atagtggagt accagcaggt tttcaacagc gccccaagag acatgtagat
     2401 atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt
     2461 gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt
     2521 aagatctcat tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg
     2581 atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta
     2641 aagataagat gggctcatag agtaaaacga ggcgagggac ctataaacct cccttcatca
     2701 tgctatttca tgatctattt tataaaataa agatgtagaa aaaagtaagc gtaataaccg
     2761 caaaacaaat gatttaaaac atggcacata atgaggagat taagttcggt ttacgtttat
     2821 tttagtacta attgtaacgt gagactacgt atcgggaatc gcctaattaa agcattaatg
     2881 cgaacctgat tagattcacc gaccctccta tcgtgtcgac ctttctgttt cttagaattt
     2941 tttggtagtc tatgtactaa taatgtcagc ttcgtattta tttcataagc aatttgcatt
     3001 tgcaatttgt tttttacttt tatttttatt gtattgtgga atgtggactc gtaccaacat
     3061 gaagttatat accaccaaaa aaattacagt tagtcaaaag attcacgagt gagagctact
     3121 tatgattgtc ttttacgtat atgtctaatt gtctatttgc tcaataatct ttgtactttc
     3181 ttttgtcgtt gataaaatca caaagttcca aaagtaatcg aatgatttgc ttttaagaaa
     3241 agaagagctc aataattcaa catatatctg tacacaatta ccgcctttga gtgagctcta
     3301 gatgccacct gacgtctaag aagaattcgt aatcatggtc atagctgttt cctgtgtgaa
     3361 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     3421 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     3481 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3541 gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc
     3601 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     3661 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     3721 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     3781 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     3841 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg
     3901 tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa
     3961 gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
     4021 cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc
     4081 atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag
     4141 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     4201 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     4261 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa
     4321 atcaccagtc tctctctaca aatctatctc tctcgagctt tcgcagatcc cggggggcaa
     4381 tgagatatgg ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4441 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4501 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4561 aatgaactcc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4621 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4681 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     4741 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     4801 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     4861 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     4921 atgcccgacg gcgaggatct cgtcgtgaca catggcgatg cctgcttgcc gaatatcatg
     4981 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5041 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5101 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5161 cgccttcttg acgagttctt ctgaagtaga tgccgaccgg atctgtcgat cgacaagctc
     5221 gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg
     5281 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     5341 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc
     5401 ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     5461 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     5521 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     5581 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     5641 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     5701 tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc
     5761 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     5821 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     5881 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     5941 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     6001 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     6061 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     6121 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     6181 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     6241 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     6301 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     6361 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     6421 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     6481 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     6541 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     6601 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     6661 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
     6721 acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6781 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6841 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6901 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6961 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7021 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7081 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7141 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7201 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7261 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7321 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7381 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7441 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7501 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7561 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7621 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7681 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     7741 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     7801 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     7861 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
     7921 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
     7981 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
     8041 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
     8101 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
     8161 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
     8221 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
     8281 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
     8341 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
     8401 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
     8461 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
     8521 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
     8581 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
     8641 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
     8701 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
     8761 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
     8821 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
     8881 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
     8941 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
     9001 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
     9061 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
     9121 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
     9181 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
     9241 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
     9301 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
     9361 cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt
     9421 tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt
     9481 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
     9541 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
     9601 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
     9661 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
     9721 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
     9781 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
     9841 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
     9901 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
     9961 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
    10021 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
    10081 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
    10141 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
    10201 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
    10261 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
    10321 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
    10381 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
    10441 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
    10501 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
    10561 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
    10621 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
    10681 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
    10741 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
    10801 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
    10861 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
    10921 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
    10981 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
    11041 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
    11101 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
    11161 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
    11221 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
    11281 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
    11341 agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc
    11401 gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct
    11461 agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg
    11521 gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg
    11581 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac
    11641 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
    11701 cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta
    11761 cccgccaata tatcctgtca aacactgata gtttaattcc cgatctagta acatagatga
    11821 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
    11881 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
    11941 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
    12001 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa
    12061 attcgagctg gtcaccaatt cacacgtgat ggtgatggtg atggctagcg ttcttgtagc
    12121 cgaaatctgg aatgttggtc cagcgctcgc gaaagacgtg cgcggcgagc ttcggcttgc
    12181 ggtcacgagt gaacacgccc ttcttgtttc cttggacgcg catcacgccc tgagaggtcg
    12241 cgaagtccgc gaagttccac gcttgctcac ccacgaagtt ctcaaactca tcgaacacga
    12301 cgtggttcgc ctggtagtac tcgacttgat attcctcggt gaacatcact ggatcaatgt
    12361 cgtgaaagcc cgcaacggtg tctgcgccgt actcagtgat catgatcggc tttcctgggc
    12421 aacgcttgtt ccacgcgtga aattcctggc ggagatggac tttggccgct tcgagatcac
    12481 cgccatcgaa gtaccatccg ttatagcgat tgagcgcgat gacgtcaatc agttcggcga
    12541 ctttgtccgt ctccggggta gccatcacaa acagcacgat cgtgaccgga cgcttctgtg
    12601 ggtcgagttc cttggtcagc tccaccaacg gcttgaagta ctcgtacgcg ccctcttcct
    12661 cagtcgccgc ctcgttggcg atgctccaca tcacgacgct tggatggttc ttgtcacgag
    12721 acaccagttc acggagaacg tcttgatggt gctcaaacgt ccgaatcttc tcccaggtac
    12781 tgacgcgctc gctgccttcg ccgagtcccg tggtggccat gaagttgagg tgcacgccaa
    12841 ctgccggagt ctcgtcgatc acgaccagac cctcgcgatc cgcaagacgc atcaactctt
    12901 cagagtacgg atagtgtgcg gtccggaagc tgttggcgcc gatccatttg aggatattga
    12961 aatccatcac attgctcgct tcgttaaagc cacggccgtt gataggagtg tcctcatgtt
    13021 tgccaaagcc cttgaagtag aacggtttgt tgttgatgag gaacttgccg tcgttgactt
    13081 ccacggtccg cacgccgaac ggctcttcat agacatcgat ggtcagtccg tcgttcacca
    13141 gttccacttt gatctggtag agatacgtgt tcagtggttc ccagaggatg acattcggaa
    13201 tctccacgtt accgctcagg ccctcggtgc ttgcgaccac tttgccttcc tcatccacga
    13261 ccgacacttt cacggtctcg gctttgcctt gaaagtccac cgtataggtc acagtcccgg
    13321 ttgggccatt gaagtcggtc acaaccgaga tgtcctcgac gtacgtaaac ggggtcgtgt
    13381 agattttcac cggacggtgc aggcctgcat agttgaagaa gtcgaagttc ggcttgttac
    13441 gaatgacttt tccgaggccc tcttcgtggc gctcgctgta cagccccacc gggagggtgc
    13501 tatcgtcgag gatgttgtcc acggcgacgg tgacgcgatt catgccatca cgcagcgagt
    13561 tgttgatttc cgcttcgaat ggcaggaatc cgcccttgtg ctccacgacc agctcaccat
    13621 tgacatagac aattgctttg tgagttgcag agccgaagcg gagcacgata cgctgatcct
    13681 tcagataggc cggcaccgtg aactcacgtt cgtaccagac atatccgata tggttgcgga
    13741 tttccttggt cacgccaatg tcattgtaac tgcttgggac ggccatacta atagtgtcgg
    13801 tcagcttgct ttcgtaccac ttctcttcca gtcctttccc gtagtccagc ttgaagttcc
    13861 agacgccatt gaggtcgaag acgccacggg tctcggtgtt gatcgggtac agactagttc
    13921 gtcggttctg taactatcat catcatcata gacacacgaa ataaagtaat cagattatca
    13981 gttaaagcta tgtaatattt acaccataac caatcaatta aaaaatagat cagtttaaag
    14041 aaagatcaaa gctcaaaaaa ataaaaagag aaaagggtcc taaccaagaa aatgaaggag
    14101 aaaaactaga aatttaccct cagatctacc atg
//
