LOCUS       sequence-358957-        2580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing mScarlet in E1.3b format.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_252
FEATURES             Location/Qualifiers
     source          1..2580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..68
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(287..391)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      494..1082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     983..1002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2006..2580,1..217,218..286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
       61 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
      121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
      181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
      241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
      301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
      361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgtca
      421 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
      481 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      601 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      721 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1081 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1141 tgttctttcc tgcgttatcc cctgattctg tggataaccg tagtcggtct cattctatgg
     1201 tcagtaaagg cgaagcagtt atcaaagagt tcatgcgctt caaagttcat atggaagggt
     1261 cgatgaacgg gcacgaattt gaaattgaag gcgaaggcga aggccgccca tatgaaggga
     1321 cccaaaccgc aaagcttaag gttactaaag gcggtccatt acccttttcg tgggacattt
     1381 taagcccaca gtttatgtac gggagtcgcg ctttcatcaa gcaccctgcg gacatcccag
     1441 attactacaa acagtctttc cccgaggggt tcaagtggga gcgcgtgatg aacttcgagg
     1501 atggcggagc cgtgacggtc acccaagata cctctttgga ggacggtacg ttgatctaca
     1561 aagtgaaatt gcgtggcacg aattttccac ctgatgggcc tgtcatgcag aaaaagacaa
     1621 tgggatggga agcttccacg gagcgccttt acccagagga cggtgttctt aaaggggata
     1681 tcaaaatggc gctgcgtctt aaagatggag gccgctacct ggcggacttc aagactactt
     1741 acaaggccaa aaaaccagtg cagatgccgg gtgcgtacaa tgtagatcgt aaattagata
     1801 ttacaagtca caatgaagat tacacggtcg tagagcagta tgagcgcagt gaggggcgtc
     1861 actctacggg cggtatggac gagttataca agtaataagt caagagacca agattatcaa
     1921 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1981 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2041 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2101 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgggac ccacgctcac
     2161 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2221 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2281 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2341 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2401 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2461 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2521 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
//
