LOCUS       sequence-358958-        2659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing LuxR .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_033
FEATURES             Location/Qualifiers
     source          1..2659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     397..416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(635..739)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      842..1430
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1331..1350
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2433..2659,1..565,566..634))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
       61 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
      481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
      541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
      601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
      721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgtcatg accaaaatcc
      781 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      841 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      901 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      961 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1021 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1081 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1141 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1201 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1261 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1321 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1381 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1441 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1501 cgttatcccc tgattctgtg gataaccgta gtcggtctcc tatgatgaaa aacataaatg
     1561 ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta
     1621 atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca
     1681 tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat
     1741 ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta
     1801 actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat
     1861 ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta
     1921 ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact
     1981 atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag
     2041 ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa
     2101 gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa
     2161 tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca
     2221 atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc
     2281 catactttaa aaattaataa cactgatagt gctagtgtag atcacttctt gagaccaaga
     2341 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2401 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2461 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2521 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca
     2581 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2641 agtggtcctg caactttat
//
