LOCUS       sequence-35896-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Rui X, Tsao J, Scheys JO, Hammer GD, Schimmer BP
  TITLE     Contributions of specificity protein-1 and steroidogenic factor 1 to
            Adcy4 expression in Y1 mouse adrenal cells.
  JOURNAL   Endocrinology. 2008 Jul . 149(7):3668-78.
  PUBMED    18388192
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology. 2008 Jul . 149(7):3668-78."
COMMENT     Sequence Label: SF-1 shRNA #2
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(148..165)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     rep_origin      254..682
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(341..360)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     551..572
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(690..710)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtggatcc tagaggtagc cagccagagg tttgatatcc
       61 gacctctggc tggctacctc tacgcttaag aataaaggat cttttatttt cattggatct
      121 gtgtgttggt tttttgtgtg cggccgccct cgactgtgcc ttctagaaga caatagcagg
      181 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
      241 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      301 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      361 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      421 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
      481 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
      541 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
      601 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
      661 taacaaaaat ttaacgcgaa ttaattcgtg gaatgtgtgt cagttagggt gtggaaagtc
      721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      781 gtgtggaaag tccccaggct ccccag
//
