LOCUS       sequence-358960-       11312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human RUNX1A gene overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11312)
  TITLE     Klusmann Lab plasmids 2022
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 
            SIN40C.TRE.HA.RUNX1A.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
FEATURES             Location/Qualifiers
     source          1..11312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(271..276,277..294,295..301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     314..336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     333..349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     368..388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        433..812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        869..1060
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(892..912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1079..1305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1306..1486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1533..1658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2151..2384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2569..2613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2826..2943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2989..3367
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2997..3015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3069..3087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3141..3159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3177..3195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3213..3231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3276..3300
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3386..3395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(4201..4775,4776..4778,4779..4787)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4368..4385)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4595..4614
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4788..5486
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELY"
     primer_bind     complement(4921..4939)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5189..5208
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5383..5406
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        5509..6008
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5544..5567)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5941..5960
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6015..6024
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6021..6023,6024..6026,6027..6737)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             6750..6806
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6807..7553
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7580..8168
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7633..7653)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8051..8062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8240..8473
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8551..8672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8588..8607)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8642..8661
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8712..8847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8867..8886)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8868..8886)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8896..8913)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8896..8912)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8905..8927)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9054..9509
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9141..9160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9351..9372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9535..9639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9640..9708,9709..10500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9858..9877)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10671..11259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11160..11179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gtttactccc
     3001 tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag aacgtatgca
     3061 gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta tcagtgatag
     3121 agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc
     3181 tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag aacgtataag
     3241 ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
     3301 gcctggagca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
     3361 tcgtaaagga tcctccggaa ccggtgccac catgggatac ccctacgacg tgcccgacta
     3421 ccgtatcccc gtagatgcca gcacgagccg ccgcttcacg ccgccttcca ccgcgctgag
     3481 cccaggcaag atgagcgagg cgttgccgct gggcgctcct gacgccggcg ctgccctggc
     3541 cggcaagctg aggagcggcg accgcagcat ggtggaggtg ctggccgacc acccgggcga
     3601 gctggtgcgc accgacagcc ccaacttcct ctgctccgtg ctgcctacgc actggcgctg
     3661 caacaagacc ctgcccatcg ctttcaaggt ggtggcccta ggggatgttc cagatggcac
     3721 tctggtcact gtgatggctg gcaatgatga aaactactcg gctgagctga gaaatgctac
     3781 cgcagccatg aagaaccagg ttgcaagatt taatgacctc aggtttgtcg gtcgaagtgg
     3841 aagagggaaa agcttcactc tgaccatcac tgtcttcaca aacccaccgc aagtcgccac
     3901 ctaccacaga gccatcaaaa tcacagtgga tgggccccga gaacctcgaa gacatcggca
     3961 gaaactagat gatcagacca agcccgggag cttgagcttc agcgagagac tgagtgaact
     4021 ggagcagctg cggcgcacag ccatgagggt cagcccacac cacccagccc ccacgcccaa
     4081 ccctcgtgcc tccctgaacc actccactgc ctttaaccct cagcctcaga gtcagatgca
     4141 ggaggaagac acagcaccct ggagatgttg aacgcgtatg catgagtaac tgaggatccg
     4201 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     4261 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     4321 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     4381 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     4441 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     4501 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     4561 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     4621 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     4681 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     4741 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg
     4801 gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg
     4861 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     4921 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     4981 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
     5041 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
     5101 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
     5161 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
     5221 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
     5281 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
     5341 agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac atcacctccc
     5401 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
     5461 tcctgtacgg catggacgag ctgtactgag acgacgcgta attctaccgg gtaggggagg
     5521 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     5581 acacaagtgg cctctggcct cgcacacatt ccacatccac cggaaggcgc caaccggctc
     5641 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     5701 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt gtcactagtc
     5761 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     5821 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     5881 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtccacc ggaggcccgg
     5941 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     6001 gcctttcggc tagcgccacc atgggatcta agggcgagga actgttcacc ggcgtggtgc
     6061 ccatcctggt ggaactggat ggcgacgtga acggccacaa gttctctgtg cggggagagg
     6121 gcgaaggcga cgccacaaat ggcaagctga ccctgaagtt catctgcacc accggcaagc
     6181 tgcccgtgcc ttggcctacc ctcgtgacca cactgaccta cggcgtgcag tgcttcagca
     6241 gataccccga ccatatgaag cggcacgact tcttcaagag cgccatgccc gagggctacg
     6301 tgcaggaacg gaccatcagc ttcaaggacg acggcaccta caagaccaga gccgaagtga
     6361 agttcgaggg cgacaccctc gtgaaccgga tcgagctgaa gggcatcgat ttcaaagagg
     6421 acggcaacat cctgggccac aagctggagt acaacttcaa cagccacaat gtgtatatca
     6481 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccggcac aacgtggaag
     6541 atggcagcgt gcagctggcc gaccactacc agcagaacac ccccatcgga gatggccccg
     6601 tgctgctgcc cgacaaccac tacctgagca cccagagcgt gctgagcaag gaccccaacg
     6661 agaagcggga ccacatggtg ctgctggaat tcgtgaccgc cgctggcatc acccacggca
     6721 tggacgagct gtataaggga ggaagcggcg ccaccaattt cagcctgctg aaacaggccg
     6781 gcgacgtgga agagaaccct ggccctatga gccggctgga caagagcaaa gtgatcaaca
     6841 gcgccctgga actgctgaac ggcgtgggca tcgagggcct gaccacccgg aagctggccc
     6901 agaaactggg cgtggaacag cccaccctgt actggcacgt gaagaacaag cgggccctgc
     6961 tggacgccct gcccatcgag atgctggacc ggcaccacac ccacagctgc cctctggaag
     7021 gcgagagctg gcaggacttc ctgcggaaca acgccaagag ctacagatgc gccctgctga
     7081 gccaccggga cggcgccaaa gtgcacctgg gcaccagacc caccgagaag cagtacgaga
     7141 cactggaaaa ccagctggcc ttcctgtgcc agcagggctt cagcctggaa aacgccctgt
     7201 acgccctgag cgccgtgggc cacttcaccc tgggctgcgt gctggaagaa caggaacacc
     7261 aggtcgccaa agaggaaaga gagacaccca ccaccgacag catgcccccc ctgctgaagc
     7321 aggccatcga gctgttcgac agacagggcg ccgagcccgc cttcctgttc ggcctggaac
     7381 tgatcatctg cggcctcgag aagcagctga agtgcgagag cggcggaccc accgacgccc
     7441 tggacgactt cgacctggac atgctgcccg ccgatgccct ggatgatttt gatctggata
     7501 tgctccctgc tgacgctctc gatgacttcg atctcgatat gctgccaggc taatgtacaa
     7561 gtgatcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7621 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7681 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7741 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7801 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7861 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7921 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7981 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8041 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8101 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8161 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8221 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     8281 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8341 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8401 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8461 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     8521 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     8581 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8641 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8701 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8761 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8821 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     8881 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8941 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9001 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     9061 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     9121 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     9181 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     9241 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     9301 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     9361 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     9421 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     9481 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     9541 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9601 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9661 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9721 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9781 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9841 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9901 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9961 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10021 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10081 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10141 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10201 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10261 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10321 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10381 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10441 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10501 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10561 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10621 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10681 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10741 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10801 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10861 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10921 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10981 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11041 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11101 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11161 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11221 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11281 ttacggttcc tggccttttg ctggcctttt gc
//
