LOCUS       sequence-358968-       12017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human RUNX1C gene overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12017)
  TITLE     Klusmann Lab plasmids 2022
REFERENCE   2  (bases 1 to 12017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 
            SIN40C.TRE.HA.RUNX1C.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
FEATURES             Location/Qualifiers
     source          1..12017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(271..276,277..294,295..301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     314..336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     333..349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     368..388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        433..812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        869..1060
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(892..912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1079..1305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1306..1486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1533..1658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2151..2384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2569..2613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2826..2943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2989..3367
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2997..3015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3069..3087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3141..3159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3177..3195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3213..3231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3276..3300
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3402..3411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3414..3440
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4906..5480,5481..5483,5484..5492)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5073..5090)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5300..5319
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5493..6191
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELY"
     primer_bind     complement(5626..5644)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5894..5913
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6088..6111
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        6214..6713
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6249..6272)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6646..6665
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6720..6729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6726..6728,6729..6731,6732..7442)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             7455..7511
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7512..8258
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8285..8873
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8338..8358)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8756..8767)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8945..9178
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9256..9377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9293..9312)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9347..9366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9417..9552
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9572..9591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9573..9591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9601..9618)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9601..9617)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9610..9632)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9759..10214
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9846..9865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10056..10077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10240..10344
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10345..10413,10414..11205)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10563..10582)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11376..11964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11865..11884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gtttactccc
     3001 tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag aacgtatgca
     3061 gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta tcagtgatag
     3121 agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc
     3181 tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag aacgtataag
     3241 ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
     3301 gcctggagca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
     3361 tcgtaaagga tcctccggaa ccggtactag tatactccgg agccaccatg ggatacccct
     3421 acgacgtgcc cgactacgct tcagacagca tatttgagtc atttccttcg tacccacagt
     3481 gcttcatgag agaatgcata cttggaatga atccttctag ggacgtccac gatgccagca
     3541 cgagccgccg cttcacgccg ccttccaccg cgctgagccc aggcaagatg agcgaggctt
     3601 tgcctctggg cgccccagac gccggcgctg ccctggccgg caagctgagg agcggcgacc
     3661 gcagcatggt ggaggtgctg gccgaccacc cgggcgagct ggtgcgcacc gacagcccca
     3721 acttcctctg ctccgtgctg cctacgcact ggcgctgcaa caagaccctg cccatcgctt
     3781 tcaaggtggt ggccctaggg gatgttccag atggcactct ggtcactgtg atggctggca
     3841 atgatgaaaa ctactcggct gagctgagaa atgctaccgc agccatgaag aaccaggttg
     3901 caagatttaa tgacctcagg tttgtcggtc gaagtggaag agggaaaagc ttcactctga
     3961 ccatcactgt cttcacaaac ccaccgcaag tcgccaccta ccacagagcc atcaaaatca
     4021 cagtggatgg gccccgagaa cctcgaagac atcggcagaa actagatgat cagaccaagc
     4081 ccgggagctt gagcttcagc gagagactga gtgaactgga gcagctgcgg cgcacagcca
     4141 tgagggtcag cccacaccac ccagccccca cgcccaaccc tcgtgcctcc ctgaaccact
     4201 ccactgcctt taaccctcag cctcagagtc agatgcagga tacaaggcag atccaaccat
     4261 ctcctccctg gagctatgac cagtcctacc aatacctggg aagcattgcc tctccttctg
     4321 tgcacccagc aacgcccatt tcacctggac gtgccagcgg catgacaacc ctctctgcag
     4381 aactttccag tcgactctca acggcacccg acctgacagc gttcagcgac ccgcgccagt
     4441 tccccgcgct gccctccatc tccgaccccc gcatgcacta tccaggcgcc ttcacctact
     4501 ccccgacgcc ggtcacctcg ggcatcggca tcggcatgtc ggccatgggc tcggccacgc
     4561 gctaccacac ctacctgccg ccgccctacc ccggctcgtc gcaagcgcag ggaggcccgt
     4621 tccaagccag ctcgccctcc taccacctgt actacggcgc ctcggccggc tcctaccagt
     4681 tctccatggt gggcggcgag cgctcgccgc cgcgcatcct gccgccctgc accaacgcct
     4741 ccaccggctc cgcgctgctc aaccccagcc tcccgaacca gagcgacgtg gtggaggccg
     4801 agggcagcca cagcaactcc cccaccaaca tggcgccctc cgcgcgcctg gaggaggccg
     4861 tgtggaggcc ctactgacgc gtatgcatga gtaactgagg atccgcccct ctccctcccc
     4921 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     4981 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     5041 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     5101 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     5161 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     5221 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     5281 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     5341 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     5401 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     5461 ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag gaggtcatca
     5521 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     5581 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     5641 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     5701 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     5761 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     5821 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     5881 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     5941 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     6001 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     6061 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     6121 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
     6181 acgagctgta ctgagacgac gcgtaattct accgggtagg ggaggcgctt ttcccaaggc
     6241 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     6301 ggcctcgcac acattccaca tccaccggaa ggcgccaacc ggctccgttc tttggtggcc
     6361 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     6421 cgtcgtgcag gacgtgacaa atggaagtag cacgtgtcac tagtctcgtg cagatggaca
     6481 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     6541 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     6601 ggctcagggg cggggcgggc gcccgaaggt ccaccggagg cccggcattc tgcacgcttc
     6661 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcggctagcg
     6721 ccaccatggg atctaagggc gaggaactgt tcaccggcgt ggtgcccatc ctggtggaac
     6781 tggatggcga cgtgaacggc cacaagttct ctgtgcgggg agagggcgaa ggcgacgcca
     6841 caaatggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccttggc
     6901 ctaccctcgt gaccacactg acctacggcg tgcagtgctt cagcagatac cccgaccata
     6961 tgaagcggca cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag gaacggacca
     7021 tcagcttcaa ggacgacggc acctacaaga ccagagccga agtgaagttc gagggcgaca
     7081 ccctcgtgaa ccggatcgag ctgaagggca tcgatttcaa agaggacggc aacatcctgg
     7141 gccacaagct ggagtacaac ttcaacagcc acaatgtgta tatcaccgcc gacaagcaga
     7201 agaacggcat caaggccaac ttcaagatcc ggcacaacgt ggaagatggc agcgtgcagc
     7261 tggccgacca ctaccagcag aacaccccca tcggagatgg ccccgtgctg ctgcccgaca
     7321 accactacct gagcacccag agcgtgctga gcaaggaccc caacgagaag cgggaccaca
     7381 tggtgctgct ggaattcgtg accgccgctg gcatcaccca cggcatggac gagctgtata
     7441 agggaggaag cggcgccacc aatttcagcc tgctgaaaca ggccggcgac gtggaagaga
     7501 accctggccc tatgagccgg ctggacaaga gcaaagtgat caacagcgcc ctggaactgc
     7561 tgaacggcgt gggcatcgag ggcctgacca cccggaagct ggcccagaaa ctgggcgtgg
     7621 aacagcccac cctgtactgg cacgtgaaga acaagcgggc cctgctggac gccctgccca
     7681 tcgagatgct ggaccggcac cacacccaca gctgccctct ggaaggcgag agctggcagg
     7741 acttcctgcg gaacaacgcc aagagctaca gatgcgccct gctgagccac cgggacggcg
     7801 ccaaagtgca cctgggcacc agacccaccg agaagcagta cgagacactg gaaaaccagc
     7861 tggccttcct gtgccagcag ggcttcagcc tggaaaacgc cctgtacgcc ctgagcgccg
     7921 tgggccactt caccctgggc tgcgtgctgg aagaacagga acaccaggtc gccaaagagg
     7981 aaagagagac acccaccacc gacagcatgc cccccctgct gaagcaggcc atcgagctgt
     8041 tcgacagaca gggcgccgag cccgccttcc tgttcggcct ggaactgatc atctgcggcc
     8101 tcgagaagca gctgaagtgc gagagcggcg gacccaccga cgccctggac gacttcgacc
     8161 tggacatgct gcccgccgat gccctggatg attttgatct ggatatgctc cctgctgacg
     8221 ctctcgatga cttcgatctc gatatgctgc caggctaatg tacaagtgat cgcgttaagt
     8281 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     8341 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     8401 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     8461 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     8521 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     8581 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     8641 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     8701 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     8761 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8821 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     8881 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8941 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     9001 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     9061 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9121 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     9181 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     9241 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9301 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9361 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     9421 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     9481 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     9541 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     9601 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     9661 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     9721 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     9781 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     9841 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9901 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9961 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    10021 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    10081 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    10141 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    10201 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    10261 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    10321 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    10381 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    10441 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    10501 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    10561 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    10621 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    10681 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    10741 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    10801 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    10861 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    10921 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    10981 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    11041 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    11101 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    11161 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    11221 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    11281 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    11341 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    11401 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    11461 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    11521 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    11581 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    11641 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    11701 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    11761 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    11821 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    11881 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11941 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    12001 ttttgctggc cttttgc
//
