LOCUS       sequence-35897-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 597 pCSmCherry
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     51..68
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    124..148
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             154..864
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(299..317)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     567..586
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     761..784
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
       61 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcaagga
      121 tcaacaagtt tgtacaaaaa agcaggctcc accatggtga gcaagggcga ggaggacaac
      181 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      241 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      301 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtcccctcag
      361 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      421 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      481 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      541 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      601 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      661 ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta caaggccaag
      721 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agctggacat cacctcccac
      781 aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca ctccaccggc
      841 ggcatggacg agctgtacaa gtaa
//
