LOCUS       sequence-358973-        8727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human RUNX1A gene overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8727)
  TITLE     Klusmann Lab plasmids 2022
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRRL.PPT.SFFV.HA.RUNX1A.IRES.eGFP
FEATURES             Location/Qualifiers
     source          1..8727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1960..2366
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2407..2416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(3222..3796,3797..3799,3800..3808)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3389..3406)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3616..3635
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3809..3811,3812..3814,3815..4528)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3809..3811,3812..3814,3815..4525)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3854..3875)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4115..4134)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4462..4483
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4760..5163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             5253..5486
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5558..5679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5595..5614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5649..5668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5719..5854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5874..5893)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5875..5893)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5903..5920)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5903..5919)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5912..5934)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6061..6516
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6148..6167)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6358..6379
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6542..6646
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6647..6715,6716..7507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6865..6884)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7678..8266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8167..8186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8420..8437
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8554..8575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8590..8595,8596..8613,8614..8620)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8628..8644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8633..8655
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8652..8668
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8652..8668
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8687..8707
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8689..8707
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag ctagctgcag taacgccatt ttgcaaggca
     1981 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2041 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2101 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2161 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2221 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2281 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2341 gtcctccgac agactgagtc ggccggtcga atcaagctta tcgataccgt cgacggatcc
     2401 accggtgcca ccatgggata cccctacgac gtgcccgact accgtatccc cgtagatgcc
     2461 agcacgagcc gccgcttcac gccgccttcc accgcgctga gcccaggcaa gatgagcgag
     2521 gcgttgccgc tgggcgctcc tgacgccggc gctgccctgg ccggcaagct gaggagcggc
     2581 gaccgcagca tggtggaggt gctggccgac cacccgggcg agctggtgcg caccgacagc
     2641 cccaacttcc tctgctccgt gctgcctacg cactggcgct gcaacaagac cctgcccatc
     2701 gctttcaagg tggtggccct aggggatgtt ccagatggca ctctggtcac tgtgatggct
     2761 ggcaatgatg aaaactactc ggctgagctg agaaatgcta ccgcagccat gaagaaccag
     2821 gttgcaagat ttaatgacct caggtttgtc ggtcgaagtg gaagagggaa aagcttcact
     2881 ctgaccatca ctgtcttcac aaacccaccg caagtcgcca cctaccacag agccatcaaa
     2941 atcacagtgg atgggccccg agaacctcga agacatcggc agaaactaga tgatcagacc
     3001 aagcccggga gcttgagctt cagcgagaga ctgagtgaac tggagcagct gcggcgcaca
     3061 gccatgaggg tcagcccaca ccacccagcc cccacgccca accctcgtgc ctccctgaac
     3121 cactccactg cctttaaccc tcagcctcag agtcagatgc aggaggaaga cacagcaccc
     3181 tggagatgtt gaacgcgtat gcatgagtaa ctgaggatcc gcccctctcc ctcccccccc
     3241 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     3301 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     3361 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     3421 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     3481 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     3541 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     3601 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     3661 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt
     3721 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     3781 aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3841 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3901 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3961 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     4021 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4081 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4141 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4201 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4261 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     4321 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4381 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4441 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4501 tctcggcatg gacgagctgt acaagtaaag cggccgcgtc gacggatccc ccgggctgca
     4561 ggaattcgag catcttaccg ccatttattc ccatatttgt tctgtttttc ttgatttggg
     4621 tatacattta aatgttaata aaacaaaatg gtggggcaat catttacatt tttagggata
     4681 tgtaattact agttcaggtg tattgccaca agacaaacat gttaagaaac tttcccgtta
     4741 tttacgctct gttcctgtta atcaacctct ggattacaaa atttgtgaaa gattgactga
     4801 tattcttaac tatgttgctc cttttacgct gtgtggatat gctgctttaa tgcctctgta
     4861 tcatgctatt gcttcccgta cggctttcgt tttctcctcc ttgtataaat cctggttgct
     4921 gtctctttat gaggagttgt ggcccgttgt ccgtcaacgt ggcgtggtgt gctctgtgtt
     4981 tgctgacgca acccccactg gctggggcat tgccaccacc tgtcaactcc tttctgggac
     5041 tttcgctttc cccctcccga tcgccacggc agaactcatc gccgcctgcc ttgcccgctg
     5101 ctggacaggg gctaggttgc tgggcactga taattccgtg gtgttgtcgg ggaagctgac
     5161 gtcctttcga attcgatatc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     5221 tcttagccac tttttaaaag aaaagggggg actggaaggg ctacgtaact cccaacgaag
     5281 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5341 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5401 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5461 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5521 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5581 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5641 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5821 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     5881 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5941 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6001 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6061 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6121 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6181 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6241 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6301 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6361 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6421 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6481 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6541 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6601 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6661 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6721 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6781 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6841 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6901 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6961 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7021 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7081 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7141 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7201 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7261 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7321 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7381 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7441 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7501 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7561 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7621 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7681 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7741 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7801 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7861 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7921 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7981 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8041 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8101 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8161 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8221 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8281 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8341 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8401 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8461 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8521 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8581 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8641 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     8701 ctaaagggaa caaaagctgg agctgca
//
