LOCUS       sequence-358974-       10959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral plasmid for inducible expression of shRNA;
            neomycin selection. See manual for detailed protocols. No further 
            technical support is available from the depositing laboratory..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Wiederschain D, Wee S, Chen L, Loo A, Yang G, Huang A, Chen Y, 
            Caponigro G, Yao YM, Lengauer C, Sellers WR, Benson JD
  TITLE     Single-vector inducible lentiviral RNAi system for oncology target 
            validation.
  JOURNAL   Cell Cycle. 2009 Feb 1. 8(3):498-504.
  PUBMED    19177017
REFERENCE   2  (bases 1 to 10959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Cycle.
            2009 Feb 1. 8(3):498-504."
COMMENT     Sequence Label: Tet-pLKO-neo
FEATURES             Location/Qualifiers
     source          1..10959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          510..1082
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1090..1109
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1137..1155
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1166..1786
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1239..1258)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    1821..2394
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1988..2005)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2215..2234
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2395..3195
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2455..2474)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3065..3084
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(3224..3323)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             3467..3700
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3772..3893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3809..3828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3863..3882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3933..4068
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4089..4107)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4117..4134)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4117..4133)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4126..4148)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4275..4730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4362..4381)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4572..4593
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4756..4860
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4861..4929,4930..5721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5079..5098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5892..6480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6381..6400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6634..6651
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6768..6789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6804..6809,6810..6827,6828..6834)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6842..6858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6847..6869
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6866..6882
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6866..6882
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6901..6921
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6903..6921
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6949..7175
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7176..7356
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7403..7528
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8021..8254
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8439..8483
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8772..8866
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     8783..8802
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    8820..8838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8848..8866
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    10784..10901
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10950..10959,1..501)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca gggggatctg tgagtttggg gacccttgat tgttctttct
      541 ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta
      601 gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact
      661 ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt
      721 cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag
      781 attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta
      841 cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc
      901 atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac atcctggtca
      961 tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat
     1021 actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc tttttcctac
     1081 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgtaat
     1141 acgactcact atagggcgaa ttgatatgtc tagattagat aaaagtaaag tgattaacag
     1201 cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca
     1261 gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct
     1321 cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg
     1381 ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag
     1441 tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac
     1501 tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata
     1561 tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca
     1621 agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca
     1681 agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt
     1741 gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt acagcggctc
     1801 ccgggagttc tagggatctg cccctctccc tccccccccc ctaacgttac tggccgaagc
     1861 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     1921 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     1981 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     2041 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     2101 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     2161 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     2221 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     2281 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     2341 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataaggatcc
     2401 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     2461 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     2521 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     2581 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     2641 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     2701 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     2761 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     2821 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     2881 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     2941 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3001 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     3061 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     3121 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     3181 gacgagttct tctgaggtac cttttttaag ggtgggcgcg ccgacccagc tttcttgtac
     3241 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     3301 agtcaaaata aaatcattat ttgccatcca gctgatatcc gcccgcccca cgacccgcag
     3361 cgcccgaccg aaaggagcgc acgaccccat gcatcggtac ctttaagacc aatgacttac
     3421 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     3481 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     3541 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3601 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3661 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     3721 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     3781 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3841 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3901 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3961 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4021 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     4081 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     4141 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4201 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4261 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4321 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4381 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4441 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4501 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4561 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4621 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4681 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4741 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4801 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4861 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4921 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4981 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5041 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5101 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5161 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5221 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5281 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5341 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5401 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5461 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5521 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5581 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5641 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5701 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5761 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5821 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6001 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6541 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6601 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6661 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6721 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6781 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6841 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     6901 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     6961 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     7021 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     7081 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     7141 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     7201 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7261 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7321 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7381 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7441 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7501 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7561 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7621 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7681 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7741 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7801 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     7861 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7921 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7981 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8041 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8101 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8161 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8221 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8281 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8341 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8401 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8461 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8521 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8581 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8641 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8701 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
     8761 gagcggccgc aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat
     8821 ccctatcagt gatagagact tataagttcc ctatcagtga tagagacacc ggtgtgttgt
     8881 aaatgagcac acaaaataca catgctaaaa tattatattc tatgaccttt ataaaatcaa
     8941 ccaaaatctt ctttttaata actttagtat caataattag aatttttatg ttcctttttg
     9001 caaactttta ataaaaatga gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt
     9061 ttcttcacct ttaataatag ctactccacc acttgttcct aagcggtcag ctcctgcttc
     9121 aatcattttt tgagcatctt caaatgttct aactccacca gctgctttaa ctaaagcatt
     9181 gtctttaaca actgacttca ttagtttaac atcttcaaat gttgcacctg attttgaaaa
     9241 tcctgttgat gttttaacaa attctaatcc agcttcaaca gctatttcac aagctttcat
     9301 gatttcttct tttgttaata aacaattttc cataatacat ttaacaacat gtgatccagc
     9361 tgcttttttt acagctttca tgtcttctaa aactaattca taatttttgt cttttaatgc
     9421 accaatattt aataccatat caatttctgt tgcaccatct ttaattgctt cagaaacttc
     9481 gaatgctttt gtagctgttg tgcatgcacc tagaggaaaa cctacaacat ttgttattcc
     9541 tacatttgtg ccttttaata attctttaca atagcttgtt caatatgaat taacacaaac
     9601 tgttgcaaaa tcaaattcaa ttgcttcatc acataattgt ttaatttcag ctttcgtagc
     9661 atcttgtttt aataatgtgt gatctatata tttgtttagt ttcatttttt ctcctatata
     9721 ttcattttta attttaattc tttaataatt tcgtctactt taactttagc gttttgaaca
     9781 gattcaccaa cacctataaa ataaattttt agtttaggtt cagttccact tgggcgaaca
     9841 gcaaatcatg acttatcttc taaataaaat tttagtaagt cttgtcctgg catattatac
     9901 attccatcga tgtagtcttc aacattaaca actttaagtc cagcaatttg agttaagggt
     9961 gttgctctca atgatttcat taatggttca atttttaatt tcttttcttc tggtttaaaa
    10021 ttcaagttta aagtgaaagt gtaatatgca cccatttctt taaataaatc ttctaaatag
    10081 tctactaatg ttttattttg ttttttataa aatcaagcag cctctgctat taatatagaa
    10141 gcttgtattc catctttatc tctagctgag tcatcaatta catatccata actttcttca
    10201 taagcaaaaa caaaatttaa tccgttatct tcttctttag caatttctct acccattcat
    10261 ttaaatccag ttaaagtttt tacaatatta actccatatt tttcatgagc gattctatca
    10321 cccaaatcac ttgttacaaa acttgaatat agagccggat tttttggaat gctatttaag
    10381 cgttttagat ttgataattt tcaatcaatt aaaattggtc ctgtttgatt tccatctaat
    10441 cttacaaaat gaccatcatg ttttattgcc attccaaatc tgtcagcatc tgggtcattc
    10501 ataataataa tatctgcatc atgtttaata ccatattcaa gcggtatttt tcatgcagga
    10561 tcaaattctg gatttggatt tacaacattt ttaaatgttt catcttcaaa tgcatgctct
    10621 tcaacctcaa taacgttata tcctgattca cgtaatattt ttggggtaaa tttagttcct
    10681 gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg ctcttttttg
    10741 tagaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
    10801 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    10861 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    10921 gcagagatcc actttggccg cggctcgagg gggttgggg
//
