LOCUS       sequence-35900-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ
  TITLE     A critical function for beta-amyloid precursor protein in neuronal 
            migration revealed by in utero RNA interference.
  JOURNAL   J Neurosci. 2007 Dec 26. 27(52):14459-69.
  PUBMED    18160654
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2007 Dec 26. 27(52):14459-69."
COMMENT     Sequence Label: pENTR APP shRNA 2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..103
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        155..403
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     155..175
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     326..345
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(460..559)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(576..595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(577..595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(600..616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(600..616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(718..737)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(796..815)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggcccaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
       61 caatgctttt ttataatgcc aactttgtac aaaaaagcag gctttaaagg aaccaattca
      121 gtcgactgga tccggtacca aggtcgggca ggaagagggc ctatttccca tgattccttc
      181 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
      241 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
      301 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
      361 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgcactaa cttgcacgac
      421 tatgcgaaca tagtcgtgca agttagtgct tttttctaga cccagctttc ttgtacaaag
      481 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
      541 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
      601 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
      661 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
      721 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
      781 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
      841 tctatcgctt
//
