LOCUS       sequence-359006-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the mammalian expression of the tardigrade protein CAHS2. This 
            is one of the bottom performing targets in our apoptosis assay (it 
            induced apoptosis)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Veling MT, Nguyen DT, Thadani NN, Oster ME, Rollins NJ, Brock KP, 
            Bethel NP, Lim S, Baker D, Way JC, Marks DS, Chang RL, Silver PA
  TITLE     Natural and Designed Proteins Inspired by Extremotolerant Organisms 
            Can Form Condensates and Attenuate Apoptosis in Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 
            10.1021/acssynbio.1c00572. Epub 2022 Feb 18.
  PUBMED    35176859
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00572"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1292-1302"
COMMENT     Sequence Label: CAHS2 (pBS0249)
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggattaca aggatgatga tgataagatg gaggccatga atatgaatat cccccgcgat
       61 gccatgtttg ttccgccacc ggaatctgag caaaatgggt atcatgagaa gtcagaagtt
      121 cagcaaacaa gttatatgca gagtcaagtc aaagtgccac attataattt cccgacacca
      181 tattttacga cttccttttc tgcgcaagag ctgctgggcg aagggtttca agcctcaatt
      241 tcccgtattt cagccgttac ggaagacatg cagagcatgg aaatcccgga gttcgttgaa
      301 gaggcccgcc gtgattacgc agccaaaaca cgtgaaaatg agatgctggg gcaacaatat
      361 gaaaaagagc tggaacgtaa gtccgaagcc taccgcaaac atcaggaagt agaggccgac
      421 aaaatccgca aagaacttga aaaacagcat atgcgtgata ttgaatttcg gaaagaaatt
      481 gcagaactgg cgattgagaa ccaaaaacgt atgatcgatc ttgaatgccg ctatgcaaaa
      541 aaagacatgg accgggaacg cacaaaagtt cgtatgatgc tggagcaaca gaaattccat
      601 agtgatatcc aggtaaatct ggattcttct gcggctggga ccgagagcgg aggtcatgta
      661 gtgagccagt ctgaaaagtt caccgaacgt aaccgcgaga tgaaacgcta a
//
