LOCUS       sequence-359010-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the mammalian expression of the tardigrade protein CAHS7_HYPDU. 
            This is one of the top performing targets in our apoptosis assay (it
            protected from apoptosis)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Veling MT, Nguyen DT, Thadani NN, Oster ME, Rollins NJ, Brock KP, 
            Bethel NP, Lim S, Baker D, Way JC, Marks DS, Chang RL, Silver PA
  TITLE     Natural and Designed Proteins Inspired by Extremotolerant Organisms 
            Can Form Condensates and Attenuate Apoptosis in Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 
            10.1021/acssynbio.1c00572. Epub 2022 Feb 18.
  PUBMED    35176859
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00572"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1292-1302"
COMMENT     Sequence Label: CAHS7_HYPDU_Boothby pSecTag (pBS0251)
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggattaca aggatgatga tgataagatg atgtttctgg cccgcaatgt aagcagagtg
       61 gctcttcgat ctgcgagcct ctctcctgca gcgatcccac agcagcagca tgcaggagtg
      121 gctgccgtct atgcggtcag gttcgcctca tcatccgggt cagggcggcc cgctgacaac
      181 tgggccgagt ctcaaaagga gaaggccaaa gccggcctca aggacgctca ggccgaggtc
      241 gggaaggtgg ctagagaagt taaggacaag gccgctggag ggatcgagca ggccaaggat
      301 gccgtgaagc agggagccaa tgacctgaaa cggtcaggga gtaggacctt tgaaaatgcc
      361 aaggatgaca ttcaggcgaa agcccagcac gcgaaatccg acctgaaggg ggcgaagcac
      421 caggccgaag gcgtcgttga aaatgtgaaa gaagcagcag aaaatgcatg ggagaagaca
      481 aaggacgtcg ctgagaacct gaaagacaag gtgcaatccc cgggtgggct cgcagacaaa
      541 gctgcgaatg cctgggagac agtaaaggat agggctcagg acgctgcctc tgaagttaag
      601 cacaaggctg gcgacctgaa agataaggcc cagcaggtaa ttcacgatgc gaccacacaa
      661 agcggagaca ataggaagca ggatcagcag caacgaagag acagccaggg ttctcagtca
      721 ggtcagaatt ctcggtctcg gaacgggtgt ttgtggtttt gttgtgtggt attgtga
//
