LOCUS       sequence-359012-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the mammalian expression of the tardigrade protein MAHS_RAMVA. 
            This is one of the bottom performing targets in our apoptosis assay 
            (it induced apoptosis)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Veling MT, Nguyen DT, Thadani NN, Oster ME, Rollins NJ, Brock KP, 
            Bethel NP, Lim S, Baker D, Way JC, Marks DS, Chang RL, Silver PA
  TITLE     Natural and Designed Proteins Inspired by Extremotolerant Organisms 
            Can Form Condensates and Attenuate Apoptosis in Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 
            10.1021/acssynbio.1c00572. Epub 2022 Feb 18.
  PUBMED    35176859
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00572"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1292-1302"
COMMENT     Sequence Label: MAHS_RAMVA_Tanaka pSecTag (pBS0253)
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggattaca aggatgatga tgataagatg atgtccagat acctgctgcg cgatgtccag
       61 gctgtattac gcggagttcg caaagtggcc gagagtagct taaagctgga gacggagaaa
      121 gtcagtctgc ggcttggtga ctttcggtca cagccttccc ttcgcagtgt gcctgcttcc
      181 ctcacaagtc gatcacaggc atttagccta caggagatag ctgctcgtgc cggagttgtt
      241 ctgcgaggag tgcaacaaca gttccgaaac gtcactggag tgaatgccgc tcctgttgta
      301 gcctttgata atggatcagt tctatacagt gaaagaatcc actcgcagag ttcgcagaag
      361 caggccccga ctacagtacc aacaggatcc gtcagcaatt cccctcaacc ggaaggaaag
      421 gcaaacgaag ctgctgaacg cgcaaaacag tttatgaatc ctccagttgc gccaatggat
      481 cctgtcgaca agaatgaatt tgtcgccatg ccggagatgg gtcgtagtaa tggaaatgga
      541 gaaaacaaac aagctgctga tttcatgaaa aaccaaggtg acaccgatat ggattcccag
      601 tacgcgcctg attcatcgaa gaacacgaaa tcggttccca cgaaggaaat cgttgctgaa
      661 gatggttcga tgagcattga ggatatcaag aaagctacgc aggttactcc tggagttgca
      721 gttaaaaacg agggtgttta ggggtgtttg tggttttgtt gtgtggtatt g
//
