LOCUS       sequence-35904-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Covassin LD, Siekmann AF, Kacergis MC, Laver E, Moore JC, Villefranc
            JA, Weinstein BM, Lawson ND
  TITLE     A genetic screen for vascular mutants in zebrafish reveals dynamic 
            roles for Vegf/Plcg1 signaling during artery development.
  JOURNAL   Dev Biol. 2009 May 15. 329(2):212-26.
  PUBMED    19269286
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2009 May 15. 329(2):212-26."
COMMENT     Sequence Label: 786 pCSmAKT2Acherry
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             165..185
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcaacaa gtttgtacaa aaaagcaggc tccgcgttta gcttatgggg agcagcaaga
      181 gcaagcccaa gtctagaatg aacgacgtag ccattgtgaa ggagggctgg ctgcacaaac
      241 gaggggaata tattaaaacc tggcggccac gctacttcct cctcaagaac gatggcacct
      301 ttattggcta caaggaacgg cctcaggatg tggatcagcg agagtcccca ctcaacaact
      361 tctcagtggc acaatgccag ctgatgaaga cagagcggcc aaggcccaac acctttatca
      421 tccgctgcct gcagtggacc acagtcattg agcgcacctt ccatgtggaa acgcctgagg
      481 agcgggaaga atgggccacc gccattcaga ctgtggccga tggactcaag aggcaggaag
      541 aagagacgat ggacttccga tcaggctcac ccagtgacaa ctcaggggct gaagagatgg
      601 aggtgtccct ggccaagccc aagcaccgtg tgaccatgaa cgagtttgag tacctgaaac
      661 tactgggcaa gggcaccttt gggaaagtga ttctggtgaa agagaaggcc acaggccgct
      721 actatgccat gaagatcctc aagaaggagg tcatcgtcgc caaggatgag gttgcccaca
      781 cgcttactga gaaccgtgtc ctgcagaact ctaggcatcc cttccttacg gccctcaagt
      841 actcattcca gacccacgac cgcctctgct ttgtcatgga gtatgccaac gggggcgagc
      901 tcttcttcca cctgtctcga gagcgcgtgt tctccgagga ccgggcccgc ttctatggtg
      961 cggaga
//
