LOCUS       sequence-359084-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the mammalian expression of the tardigrade protein CAHS6_HYPDU. 
            This is one of the top performing targets in our apoptosis assay (it
            protected from apoptosis)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Veling MT, Nguyen DT, Thadani NN, Oster ME, Rollins NJ, Brock KP, 
            Bethel NP, Lim S, Baker D, Way JC, Marks DS, Chang RL, Silver PA
  TITLE     Natural and Designed Proteins Inspired by Extremotolerant Organisms 
            Can Form Condensates and Attenuate Apoptosis in Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 
            10.1021/acssynbio.1c00572. Epub 2022 Feb 18.
  PUBMED    35176859
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00572"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1292-1302"
COMMENT     Sequence Label: pST_CAHS6_HYPDU (pBS0331)
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             694..717
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgagcggca gaaacgtgga gtctcatatg gagcgcaacg agaaagtggt cgtgaataac
       61 tctggacacg cagacgtgaa gaaacagcag cagcaggtgg aacataccga gtttactcat
      121 accgaagtga aagcccctct gatccaccca gcccccccta tcatcagcac cggggccgcc
      181 gggctggccg aggagattgt gggccagggc ttcactgcca gcgccgccag aatcagcggg
      241 ggcacagccg aagtacacct gcagcccagc gcagctatga ccgaggaggc cagaagagac
      301 caggagagat acaggcagga acaggagtcc atcgccaagc agcaggagcg tgagatggag
      361 aagaagaccg aggcctacag aaagaccgcc gaggccgagg ctgagaagat ccgcaaggag
      421 ctggagaagc agcacgcccg cgacgtggag ttcagaaagg acctgatcga gagcaccatc
      481 gacagacaga agagagaggt ggacctggag gctaagatgg ccaagagaga gctggacaga
      541 gagggccagc tggccaagga ggcactggag agaagcagac tggccaccaa cgtggaggtg
      601 aacttcgaca gcgccgccgg ccatacagtg tctgggggca ccacagtgag cacctctgat
      661 aagatggaga tcaagagaaa ctgaggatct ggggactaca aggacgacga cgacaagtga
//
