LOCUS       sequence-35909-e        5020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a phytochrome-based near-infrared fluorescent protein, also known as
            iRFP713, used for in vivo imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV
  TITLE     Bright and stable near-infrared fluorescent protein for in vivo 
            imaging.
  JOURNAL   Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.
  PUBMED    21765402
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918."
COMMENT     Sequence Label: pBAD/His-B-iRFP
FEATURES             Location/Qualifiers
     source          1..5020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..59)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      212..298
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      390..417
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        437..528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(529..597,598..1389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(747..766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1560..2148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2049..2068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2302..2319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2334..2474
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2400..2419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2560..2582
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2738..3616)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3643..3927
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3850..3869
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             3974..3991
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3995..4027
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(4031..4039,4040..4054)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(4073..5020,1..3)
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MTEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
ORIGIN
        1 tgaaagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
       61 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
      121 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
      181 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      241 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      301 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
      361 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      421 gtttctacaa actctttttg tttatttttc taaatacatt caaatatgta tccgctcatg
      481 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      541 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      601 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      661 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      721 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
      781 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      841 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      901 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      961 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1021 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1081 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1141 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1201 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1261 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1321 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1381 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1441 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1501 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1561 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1621 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1681 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1741 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1801 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1861 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1921 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1981 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2041 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2101 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2161 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2221 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2281 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2341 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2401 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     2461 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2521 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2581 ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga
     2641 agcggcatgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
     2701 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
     2761 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     2821 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     2881 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
     2941 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
     3001 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     3061 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
     3121 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     3181 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
     3241 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
     3301 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     3361 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     3421 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     3481 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
     3541 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
     3601 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
     3661 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg
     3721 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
     3781 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
     3841 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     3901 tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctaacagg aggaattaac
     3961 catggggggt tctcatcatc atcatcatca tggtatggct agcatgactg gtggacagca
     4021 aatgggtcgg gatctgtacg acgatgacga taaggatccg agctcgagat ctatgacaga
     4081 aggatccgtc gccaggcagc ctgacctctt gacctgcgac gatgagccga tccatatccc
     4141 cggtgccatc caaccgcatg gactgctgct cgccctcgcc gccgacatga cgatcgttgc
     4201 cggcagcgac aaccttcccg aactcaccgg actggcgatc ggcgccctga tcggccgctc
     4261 tgcggccgat gtcttcgact cggagacgca caaccgtctg acgatcgcct tggccgagcc
     4321 cggggcggcc gtcggagcac cgatcactgt cggcttcacg atgcgaaagg acgcaggctt
     4381 catcggctcc tggcatcgcc atgatcagct catcttcctc gagctcgagc ctccccagcg
     4441 ggacgtcgcc gagccgcagg cgttcttccg ccgcaccaac agcgccatcc gccgcctgca
     4501 ggccgccgaa accttggaaa gcgcctgcgc cgccgcggcg caagaggtgc ggaagattac
     4561 cggcttcgat cgggtgatga tctatcgctt cgcctccgac ttcagcggcg aagtgatcgc
     4621 agaggatcgg tgcgccgagg tcgagtcaaa actaggcctg cactatcctg cctcaaccgt
     4681 gccggcgcag gcccgtcggc tctataccat caacccggta cggatcattc ccgatatcaa
     4741 ttatcggccg gtgccggtca ccccagacct caatccggtc accgggcggc cgattgatct
     4801 tagcttcgcc atcctgcgca gcgtctcgcc cgtccatctg gaattcatgc gcaacatagg
     4861 catgcacggc acgatgtcga tctcgatttt gcgcggcgag cgactgtggg gattgatcgt
     4921 ttgccatcac cgaacgccgt actacgtcga tctcgatggc cgccaagcct gcgagctagt
     4981 cgcccaggtt ctggcctggc agatcggcgt gatggaagag
//
