LOCUS       sequence-35914-e        8314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a phytochrome-based near-infrared fluorescent protein, also known as
            iRFP713, used for in vivo imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV
  TITLE     Bright and stable near-infrared fluorescent protein for in vivo 
            imaging.
  JOURNAL   Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.
  PUBMED    21765402
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918."
COMMENT     Sequence Label: pShuttle-CMV-iRFP
FEATURES             Location/Qualifiers
     source          1..8314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        409..712
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        713..916
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     867..887
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      953..962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             959..1909
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     polyA_signal    2081..2202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2118..2137)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2172..2191
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5536..5553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5707..6295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5787..5806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7106..7900
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7106..7900
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7160..7179)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7770..7789
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8010..8031)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8222..8241
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaattaann ntcccttcca gctctctgcc ccttttggat tgaagccaat atgataatga
       61 gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt
      121 ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag
      181 tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc
      241 ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact
      301 gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta annnntaata
      361 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      421 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      481 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      541 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      601 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      661 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      721 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      781 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      841 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      901 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagagatctg ccgccaccat
      961 ggcggaagga tccgtcgcca ggcagcctga cctcttgacc tgcgacgatg agccgatcca
     1021 tatccccggt gccatccaac cgcatggact gctgctcgcc ctcgccgccg acatgacgat
     1081 cgttgccggc agcgacaacc ttcccgaact caccggactg gcgatcggcg ccctgatcgg
     1141 ccgctctgcg gccgatgtct tcgactcgga gacgcacaac cgtctgacga tcgccttggc
     1201 cgagcccggg gcggccgtcg gagcaccgat cactgtcggc ttcacgatgc gaaaggacgc
     1261 aggcttcatc ggctcctggc atcgccatga tcagctcatc ttcctcgagc tcgagcctcc
     1321 ccagcgggac gtcgccgagc cgcaggcgtt cttccgccgc accaacagcg ccatccgccg
     1381 cctgcaggcc gccgaaacct tggaaagcgc ctgcgccgcc gcggcgcaag aggtgcggaa
     1441 gattaccggc ttcgatcggg tgatgatcta tcgcttcgcc tccgacttca gcggcgaagt
     1501 gatcgcagag gatcggtgcg ccgaggtcga gtcaaaacta ggcctgcact atcctgcctc
     1561 aaccgtgccg gcgcaggccc gtcggctcta taccatcaac ccggtacgga tcattcccga
     1621 tatcaattat cggccggtgc cggtcacccc agacctcaat ccggtcaccg ggcggccgat
     1681 tgatcttagc ttcgccatcc tgcgcagcgt ctcgcccgtc catctggaat tcatgcgcaa
     1741 cataggcatg cacggcacga tgtcgatctc gattttgcgc ggcgagcgac tgtggggatt
     1801 gatcgtttgc catcaccgaa cgccgtacta cgtcgatctc gatggccgcc aagcctgcga
     1861 gctagtcgcc caggttctgg cctggcagat cggcgtgatg gaagagtgag tcgacgcggc
     1921 cgctcgagcc taagcttcta gataagatat ccgatccacc ggatctagat aactgatcat
     1981 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     2041 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     2101 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2161 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcnnnn taagggtggg
     2221 aaagaatata taaggtgggg gtcttatgta gttttgtatc tgttttgcag cagccgccgc
     2281 cgccatgagc accaactcgt ttgatggaag cattgtgagc tcatatttga caacgcgcat
     2341 gcccccatgg gccggggtgc gtcagaatgt gatgggctcc agcattgatg gtcgccccgt
     2401 cctgcccgca aactctacta ccttgaccta cgagaccgtg tctggaacgc cgttggagac
     2461 tgcagcctcc gccgccgctt cagccgctgc agccaccgcc cgcgggattg tgactgactt
     2521 tgctttcctg agcccgcttg caagcagtgc agcttcccgt tcatccgccc gcgatgacaa
     2581 gttgacggct cttttggcac aattggattc tttgacccgg gaacttaatg tcgtttctca
     2641 gcagctgttg gatctgcgcc agcaggtttc tgccctgaag gcttcctccc ctcccaatgc
     2701 ggtttaaaac ataaataaaa aaccagactc tgtttggatt tggatcaagc aagtgtcttg
     2761 ctgtctttat ttaggggttt tgcgcgcgcg gtaggcccgg gaccagcggt ctcggtcgtt
     2821 gagggtcctg tgtatttttt ccaggacgtg gtaaaggtga ctctggatgt tcagatacat
     2881 gggcataagc ccgtctctgg ggtggaggta gcaccactgc agagcttcat gctgcggggt
     2941 ggtgttgtag atgatccagt cgtagcagga gcgctgggcg tggtgcctaa aaatgtcttt
     3001 cagtagcaag ctgattgcca ggggcaggcc cttggtgtaa gtgtttacaa agcggttaag
     3061 ctgggatggg tgcatacgtg gggatatgag atgcatcttg gactgtattt ttaggttggc
     3121 tatgttccca gccatatccc tccggggatt catgttgtgc agaaccacca gcacagtgta
     3181 tccggtgcac ttgggaaatt tgtcatgtag cttagaagga aatgcgtgga agaacttgga
     3241 gacgcccttg tgacctccaa gattttccat gcattcgtcc ataatgatgg caatgggccc
     3301 acgggcggcg gcctgggcga agatatttct gggatcacta acgtcatagt tgtgttccag
     3361 gatgagatcg tcataggcca tttttacaaa gcgcgggcgg agggtgccag actgcggtat
     3421 aatggttcca tccggcccag gggcgtagtt accctcacag atttgcattt cccacgcttt
     3481 gagttcagat ggggggatca tgtctacctg cggggcgatg aagaaaacgg tttccggggt
     3541 aggggagatc agctgggaag aaagcaggtt cctgagcagc tgcgacttac cgcagccggt
     3601 gggcccgtaa atcacaccta ttaccgggtg caactggtag ttaagagagc tgcagctgcc
     3661 gtcatccctg agcagggggg ccacttcgtt aagcatgtcc ctgactcgca tgttttccct
     3721 gaccaaatcc gccagaaggc gctcgccgcc cagcgatagc agttcttgca aggaagcaaa
     3781 gtttttcaac ggtttgagac cgtccgccgt aggcatgctt ttgagcgttt gaccaagcag
     3841 ttccaggcgg tcccacagct cggtcacctg ctctacggca tctcgatcca gcatatctcc
     3901 tcgtttcgcg ggttggggcg gctttcgctg tacggcagta gtcggtgctc gtccagacgg
     3961 gccagggtca tgtctttcca cgggcgcagg gtcctcgtca gcgtagtctg ggtcacggtg
     4021 aaggggtgcg ctccgggctg cgcgctggcc agggtgcgct tgaggctggt cctgctggtg
     4081 ctgaagcgct gccggtcttc gccctgcgcg tcggccaggt agcatttgac catggtgtca
     4141 tagtccagcc cctccgcggc gtggcccttg gcgcgcagct tgcccttgga ggaggcgccg
     4201 cacgaggggc agtgcagact tttgagggcg tagagcttgg gcgcgagaaa taccgattcc
     4261 ggggagtagg catccgcgcc gcaggccccg cagacggtct cgcattccac gagccaggtg
     4321 agctctggcc gttcggggtc aaaaaccagg tttcccccat gctttttgat gcgtttctta
     4381 cctctggttt ccatgagccg gtgtccacgc tcggtgacga aaaggctgtc cgtgtccccg
     4441 tatacagact nnngtttaaa cgaattcnnn atataaaatg caaggtgctg ctcaaaaaat
     4501 caggcaaagc ctcgcgcaaa aaagaaagca catcgtagtc atgctcatgc agataaaggc
     4561 aggtaagctc cggaaccacc acagaaaaag acaccatttt tctctcaaac atgtctgcgg
     4621 gtttctgcat aaacacaaaa taaaataaca aaaaaacatt taaacattag aagcctgtct
     4681 tacaacagga aaaacaaccc ttataagcat aagacggact acggccatgc cggcgtgacc
     4741 gtaaaaaaac tggtcaccgt gattaaaaag caccaccgac agctcctcgg tcatgtccgg
     4801 agtcataatg taagactcgg taaacacatc aggttgattc atcggtcagt gctaaaaagc
     4861 gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca
     4921 taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct
     4981 gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttca cagcggcagc
     5041 ctaacagtca gccttaccag taaaaaagaa aacctattaa aaaaacacca ctcgacacgg
     5101 caccagctca atcagtcaca gtgtaaaaaa gggccaagtg cagagcgagt atatatagga
     5161 ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc cagaaaaccg cacgcgaacc
     5221 tacgcccaga aacgaaagcc aaaaaaccca caacttcctc aaatcgtcac ttccgttttc
     5281 ccacgttacg taacttccca ttttaagaaa actacaattc ccaacacata caagttactc
     5341 cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc gcgccacgtc acaaactcca
     5401 ccccctcatt atcatattgg cttcaatcca aaataaggta tattattgat gatnnnttaa
     5461 ttaaggatcc nnncggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     5521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5881 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6121 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgnnnnaaa
     6781 aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc
     6841 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa
     6901 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     6961 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     7021 aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca
     7081 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     7141 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     7201 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     7261 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     7321 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     7381 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     7441 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     7501 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     7561 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     7621 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     7681 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     7741 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     7801 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     7861 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt
     7921 aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat aaatcaaaag
     7981 aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     8041 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     8101 aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta aatcggaacc
     8161 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     8221 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     8281 gcgtaaccac cacacccgcg cgcttaatgc gccg
//
