LOCUS       sequence-35924-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET mCerulean LIC cloning vector (H6-mCerulean)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(65..778,779..781))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     HFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(110..131)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     459..478
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     718..739
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(818..838)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(839..856)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 cttactactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      121 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca
      181 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      241 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag tgggccttga
      301 tgccgttctt ctgcttgtcg gcggtgatat agacgttgtc gctgatggcg ttgtactcca
      361 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
      421 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
      481 tgaagaagat ggtacgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
      541 gctgcttcat gtggtcgggg tagcgggcga agcactgcac gccccaggtc agggtggtca
      601 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
      661 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacctgtgg ccgtttacgt
      721 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
      781 cggatccgct gctgccgttg ctcccactac caatattgga ttggaagtac aggttttcat
      841 ggtgatggtg atggtgttca tgccattcaa ttttctgcgc ttcaaaa
//
