LOCUS       sequence-35932-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Kim SH, Li Z, Sacks DB
  TITLE     E-cadherin-mediated cell-cell attachment activates Cdc42.
  JOURNAL   J Biol Chem. 2000 Nov 24. 275(47):36999-7005.
  PUBMED    10950951
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Nov 24. 275(47):36999-7005."
COMMENT     Sequence Label: pGEX-KG-WASP-GBD
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(338..360)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(505..523)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatcggatct ggttccgcgt ggatcctcag ggaagaagaa gatcagcaaa gctgatattg
       61 gtgcacccag tggattcaag catgtcagcc acgtggggtg ggacccccag aatggatttg
      121 acgtgaacaa cctcgaccca gatctgcgga gtctgttctc cagggcagga atcagcgagg
      181 cccagctcac cgacgccgag acctctaaac ttatctacga cttcattgag gaccagggtt
      241 aagaattcta gactccatgg gtcgactcga gctcaagctt aattcatcgt gactgactga
      301 cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      361 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
      421 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
      481 tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
//
