LOCUS       sequence-35938-e         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  TITLE     TALE Nuclease (TALEN)
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TALEN Backbone
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             76..141
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             148..168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 ataggggcca ccatggacta taaggaccac gacggagact acaaggatca tgatattgat
      121 tacaaagacg atgacgataa gatggcccca aagaagaagc ggaaggtcgg tatccacgga
      181 gtcccagcag ccgtagattt gagaactttg ggatattcac agcagcagca ggaaaagatc
      241 aagcccaaag tgaggtcgac agtcgcgcag catcacgaag cgctggtggg tcatgggttt
      301 acacatgccc acatcgtagc cttgtcgcag caccctgcag cccttggcac ggtcgccgtc
      361 aagtaccagg acatgattgc ggcgttgccg gaagccacac atgaggcgat cgtcggtgtg
      421 gggaaacagt ggagcggagc ccgagcgctt gaggccctgt tgacggtcgc gggagagctg
      481 agagggcctc cccttcagct ggacacgggc cagttgctga agatcgcgaa gcggggagga
      541 gtcacggcgg tcgaggcggt gcacgcgtgg cgcaatgcgc tcacgggagc acccctcaac
      601 ctgaccgaga ccgtacatga aa
//
