LOCUS       sequence-359388-        8694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Hsu SC, Hung MC
  TITLE     Characterization of a novel tripartite nuclear localization sequence
            in the EGFR family.
  JOURNAL   J Biol Chem. 2007 Apr 6;282(14):10432-40. Epub 2007 Feb 5.
  PUBMED    17283074
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-04-6"; volume: "282(14)"; pages: "10432-40. Epub
            2007 Feb 5"
COMMENT     Sequence Label: pCDNA6A-EGFR WT
FEATURES             Location/Qualifiers
     source          1..8694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(686..757,758..2620,2621..2689,2690..4315)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             4331..4360
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4376..4393
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4416..4433)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4422..4646
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4692..5120
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4779..4798)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4989..5010
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5129..5149)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5134..5463
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5314..5449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5376..5395
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5511..5558
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5577..5975
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6133..6254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6170..6189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6224..6243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6303..6319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6303..6319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6316..6338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6327..6343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6351..6357,6358..6375,6376..6381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6396..6417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6534..6551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6705..7293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6785..6804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7464..8255,8256..8324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8325..8429)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8504..8523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagttaag cttcggggag cagcgatgcg accctccggg acggccgggg cagcgctcct
      721 ggcgctgctg gctgcgctct gcccggcgag tcgggctctg gaggaaaaga aagtttgcca
      781 aggcacgagt aacaagctca cgcagttggg cacttttgaa gatcattttc tcagcctcca
      841 gaggatgttc aataactgtg aggtggtcct tgggaatttg gaaattacct atgtgcagag
      901 gaattatgat ctttccttct taaagaccat ccaggaggtg gctggttatg tcctcattgc
      961 cctcaacaca gtggagcgaa ttcctttgga aaacctgcag atcatcagag gaaatatgta
     1021 ctacgaaaat tcctatgcct tagcagtctt atctaactat gatgcaaata aaaccggact
     1081 gaaggagctg cccatgagaa atttacagga aatcctgcat ggcgccgtgc ggttcagcaa
     1141 caaccctgcc ctgtgcaacg tggagagcat ccagtggcgg gacatagtca gcagtgactt
     1201 tctcagcaac atgtcgatgg acttccagaa ccacctgggc agctgccaaa agtgtgatcc
     1261 aagctgtccc aatgggagct gctggggtgc aggagaggag aactgccaga aactgaccaa
     1321 aatcatctgt gcccagcagt gctccgggcg ctgccgtggc aagtccccca gtgactgctg
     1381 ccacaaccag tgtgctgcag gctgcacagg cccccgggag agcgactgcc tggtctgccg
     1441 caaattccga gacgaagcca cgtgcaagga cacctgcccc ccactcatgc tctacaaccc
     1501 caccacgtac cagatggatg tgaaccccga gggcaaatac agctttggtg ccacctgcgt
     1561 gaagaagtgt ccccgtaatt atgtggtgac agatcacggc tcgtgcgtcc gagcctgtgg
     1621 ggccgacagc tatgagatgg aggaagacgg cgtccgcaag tgtaagaagt gcgaagggcc
     1681 ttgccgcaaa gtgtgtaacg gaataggtat tggtgaattt aaagactcac tctccataaa
     1741 tgctacgaat attaaacact tcaaaaactg cacctccatc agtggcgatc tccacatcct
     1801 gccggtggca tttaggggtg actccttcac acatactcct cctctggatc cacaggaact
     1861 ggatattctg aaaaccgtaa aggaaatcac agggtttttg ctgattcagg cttggcctga
     1921 aaacaggacg gacctccatg cctttgagaa cctagaaatc atacgcggca ggaccaagca
     1981 acatggtcag ttttctcttg cagtcgtcag cctgaacata acatccttgg gattacgctc
     2041 cctcaaggag ataagtgatg gagatgtgat aatttcagga aacaaaaatt tgtgctatgc
     2101 aaatacaata aactggaaaa aactgtttgg gacctccggt cagaaaacca aaattataag
     2161 caacagaggt gaaaacagct gcaaggccac aggccaggtc tgccatgcct tgtgctcccc
     2221 cgagggctgc tggggcccgg agcccaggga ctgcgtctct tgccggaatg tcagccgagg
     2281 cagggaatgc gtggacaagt gcaaccttct ggagggtgag ccaagggagt ttgtggagaa
     2341 ctctgagtgc atacagtgcc acccagagtg cctgcctcag gccatgaaca tcacctgcac
     2401 aggacgggga ccagacaact gtatccagtg tgcccactac attgacggcc cccactgcgt
     2461 caagacctgc ccggcaggag tcatgggaga aaacaacacc ctggtctgga agtacgcaga
     2521 cgccggccat gtgtgccacc tgtgccatcc aaactgcacc tacggatgca ctgggccagg
     2581 tcttgaaggc tgtccaacga atgggcctaa gatcccgtcc atcgccactg ggatggtggg
     2641 ggccctcctc ttgctgctgg tggtggccct ggggatcggc ctcttcatgc gaaggcgcca
     2701 catcgttcgg aagcgcacgc tgcggaggct gctgcaggag agggagcttg tggagcctct
     2761 tacacccagt ggagaagctc ccaaccaagc tctcttgagg atcttgaagg aaactgaatt
     2821 caaaaagatc aaagtgctgg gctccggtgc gttcggcacg gtgtataagg gactctggat
     2881 cccagaaggt gagaaagtta aaattcccgt cgctatcaag gaattaagag aagcaacatc
     2941 tccgaaagcc aacaaggaaa tcctcgatga agcctacgtg atggccagcg tggacaaccc
     3001 ccacgtgtgc cgcctgctgg gcatctgcct cacctccacc gtgcaactca tcacgcagct
     3061 catgcccttc ggctgcctcc tggactatgt ccgggaacac aaagacaata ttggctccca
     3121 gtacctgctc aactggtgtg tgcagatcgc aaagggcatg aactacttgg aggaccgtcg
     3181 cttggtgcac cgcgacctgg cagccaggaa cgtactggtg aaaacaccgc agcatgtcaa
     3241 gatcacagat tttgggctgg ccaaactgct gggtgcggaa gagaaagaat accatgcaga
     3301 aggaggcaaa gtgcctatca agtggatggc attggaatca attttacaca gaatctatac
     3361 ccaccagagt gatgtctgga gctacggggt gaccgtttgg gagttgatga cctttggatc
     3421 caagccatat gacggaatcc ctgccagcga gatctcctcc atcctggaga aaggagaacg
     3481 cctccctcag ccacccatat gtaccatcga tgtctacatg atcatggtca agtgctggat
     3541 gatagacgca gatagtcgcc caaagttccg tgagttgatc atcgaattct ccaaaatggc
     3601 ccgagacccc cagcgctacc ttgtcattca gggggatgaa agaatgcatt tgccaagtcc
     3661 tacagactcc aacttctacc gtgccctgat ggatgaagaa gacatggacg acgtggtgga
     3721 tgccgacgag tacctcatcc cacagcaggg cttcttcagc agcccctcca cgtcacggac
     3781 tcccctcctg agctctctga gtgcaaccag caacaattcc accgtggctt gcattgatag
     3841 aaatgggctg caaagctgtc ccatcaagga agacagcttc ttgcagcgat acagctcaga
     3901 ccccacaggc gccttgactg aggacagcat agacgacacc ttcctcccag tgcctgaata
     3961 cataaaccag tccgttccca aaaggcccgc tggctctgtg cagaatcctg tctatcacaa
     4021 tcagcctctg aaccccgcgc ccagcagaga cccacactac caggaccccc acagcactgc
     4081 agtgggcaac cccgagtatc tcaacactgt ccagcccacc tgtgtcaaca gcacattcga
     4141 cagccctgcc cactgggccc agaaaggcag ccaccaaatt agcctggaca accctgacta
     4201 ccagcaggac ttctttccca aggaagccaa gccaaatggc atctttaagg gctccacagc
     4261 tgaaaatgca gaatacctaa gggtcgcgcc acaaagcagt gaatttattg gagcatctag
     4321 agggcccttc gaacaaaaac tcatctcaga agaggatctg aatatgcata ccggtcatca
     4381 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     4441 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4501 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4561 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4621 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4681 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4741 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4801 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4861 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4921 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4981 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5041 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5101 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5161 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5221 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5281 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5341 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5401 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5461 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     5521 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     5581 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     5641 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     5701 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     5761 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     5821 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     5881 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     5941 aattgctgcc ctctggttat gtgtgggagg gctaagcact tcgtggccga ggagcaggac
     6001 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6061 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6121 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6241 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8461 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     8521 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     8581 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     8641 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
