LOCUS       sequence-359390-        2562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing eforRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_345
FEATURES             Location/Qualifiers
     source          1..2562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1043..1631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1123..1142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1734..1838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1839..1907,1908..2562,1..137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2057..2076)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
       61 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
      121 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
      181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat cttggtctca
      241 agaactagta tgggagagcc ttcggcagcg gtgagaaatg gccttcggct gcctcctgct
      301 ggtcccaagt tgtgaaatct ttatcgtgtt tggtcagttc caggcgatgt tcaacgaaat
      361 gaaattctgg cagcttgaca ggttttttgg ctttatacgt cgttttgaaa tcaacacgat
      421 gatgaccgcc tcctttcagt ttaaaaacca ttgcagtgtc actttttaac atcccgtcac
      481 gcacgtacag tgtttccacg ctcggctccc atccagcaat ggttttttgc attacgggcc
      541 catccggagg caggttcaca ccatgaaatt taacatcata gatgaaacag ttctcttgca
      601 agtttatgct ggagtgatta ctggcggtgc atacgccacc atcttcaaaa atcatcgacc
      661 gctcccaaga cataccttcg ggaaaagact gtttgtggta gtctgggata tccgctggat
      721 acttattaaa cggtttgctt ccatacatgt ggctaggtgt aagaatatga acggaaaacg
      781 gcagaggcgc gcctttggtg actgtcattt tcatgtgctg taacccttcg tacggcttgc
      841 cttcaccttt accctcgatc gtaaaatcat ggccattgac agtgccctca aggtgcaact
      901 tggtcttcat tacctgctta atcactgaca taggagaccg actacggtta tccacagaat
      961 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1021 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1081 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1141 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1201 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1261 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1321 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1381 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1441 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1501 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1561 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1621 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1681 actcacgtta agggattttg gtcatgacag gtggcacttt tcggggaaat gtgcgcggaa
     1741 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1801 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1861 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1921 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1981 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2041 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2101 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2161 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2221 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2281 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2341 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2401 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2461 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2521 ttattgctga taaatctgga gccggtgagc gtgggtcccg cg
//
