LOCUS       sequence-359396-        2072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing K.rhaeticus native promoter pRutB (200 bases
            upstream from gene start codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_226
FEATURES             Location/Qualifiers
     source          1..2072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(222..810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(302..321)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        913..1017
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1018..1086,1087..1878)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1236..1255)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcgtcggggc cgccatcgct gcccaaacgg caccaccgtg cgctggaaac accgcttgcc
       61 gctacacagg ccgctgtttc atgctctggg gaaatgcaag ggaaatgggt cagagaccga
      121 ctacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      601 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      661 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      721 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      781 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      841 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgacagg tggcactttt
      901 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      961 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1021 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1081 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1141 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1201 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1261 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1321 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1381 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1441 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1501 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1561 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1621 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1681 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcccgc
     1741 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1801 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1861 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1921 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tcttggtctc
     1981 agccagcaga aggatcggca ggtttactgg ccggcacaag tgctctttcc agcgcggcgt
     2041 ggtgccatat gggcatcatg cccggcgcca ta
//
