LOCUS       sequence-359419-        8322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8322)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0274M
FEATURES             Location/Qualifiers
     source          1..8322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3913..3930)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3919..4143
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4189..4617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4276..4295)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4486..4507
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4626..4646)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4631..4960
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4811..4946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4873..4892
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5027..5821
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5081..5100)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5691..5710
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5995..6116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6032..6051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6086..6105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6165..6181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6165..6181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6178..6200)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6189..6205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6213..6219,6220..6237,6238..6243))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6258..6279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6396..6413)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6567..7155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6647..6666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7326..8117,8118..8186))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7949..7968
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8187..8291)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgctgacaag gtaaagatcg acctttttat
      961 tgtatactga acaatgcgtg tttacaatgg tgtaggtggg agcagagttc gccaagctct
     1021 acgtccgaac agtcgggtgt cagggctctt attaagtgtt cggtgtactt gaccaaagcc
     1081 gcggaaccta ggttgggtct gtacaggtcg taccaggcaa aaaaggatcg ggcggtgctt
     1141 ttcaggagag ttagggacgt gctgattatg tggacaagct tctgctcgta aatgcaccgc
     1201 tggtacatct gaacgacagc tgtccaaaaa aaacaaaggt tcagctgcac gttaaaatct
     1261 gtatcctgaa agtcctcgta aatgacagtt tctaccaaga aaaacttttt taccacgctg
     1321 gccatccact gaaaggaggg agcacacgtc ccgttgtgcg ttgttaggat atccctaact
     1381 tcggagcgga gacggccgga cgctcccaca aaatgggaga ggcaccactc tgtgcagtcc
     1441 gcggtcgggg gttctgattc caggggcgcc gtgtgggggt attggagagt caaaactctg
     1501 ggcagtccct taatgagctc tctctcaaaa cctatgcagc cagcgtccac tagtggcagc
     1561 atgccgttaa taacacccct tatcttgtcg ttgccaagtt tgtacaactg ctgcagggaa
     1621 taagccaaat tcgccctagc cgcgggaacc aggtacggct cgctttgtcg gtgctggacc
     1681 aatatctgaa tggtctttgc aaggtatagg gtcttctcaa cgtttagagc gggtacgtgg
     1741 cagtctggat tgagggtggc gacggacagg gtatctaact cctgaagtat ctgatcccag
     1801 gacgggtaat gatacctaaa cagatggttg aacaggtgat ctttaagggg ccttctcgat
     1861 gtcattgtaa aaactatgac acgccactct ctccttaggg taagaagctt cggcggtcct
     1921 gtgtggaaag cttcgtcggc ctctcggacg aactgaaggc ccaactctac cagtgtgtgc
     1981 tccttataaa tgacgcatac gaaacaatct acgatcccag tgacctaaat agagtggtgg
     2041 aagatgtgtg cattcggatt atgaaagaat gttccaagct tggtgcgcta tgtggtctgt
     2101 ttacagacat taacatgttt aaccttttct gcttttttcg tgcctctcga atgaggacca
     2161 aaggcgcggc cgggtacaac gtgccatgcg cagaggcatc ccaaggcatt attcggatcc
     2221 tcacggagag gatcttattc tgcacagaaa aggcatttct gacagccgca tgcagcgggg
     2281 tgagcctgcc tccagccata tgtaagctac tacacgaaat atacactgaa atgaaggcca
     2341 aatgcctggg ggcctggagg cgactcgtct gcaatcggag gcccattatg atattaacct
     2401 cttccctact gaagctctac aacacgtacg ataccgccgg gctgctctct gagcagtcca
     2461 gggccctctg ccttttggtt ttccaaccgg tctaccttcc gaggattatg gcgccgctgg
     2521 agatcatgac caagggtcag ctcgcccctg aaaactttta cagcatcacc ggttctgctg
     2581 agaaacgccg gccaattacc accggcaagg tcactggact gtcctatcca ggaagcggtc
     2641 tcatgccaga atctttaatt ttgccaatcc tggagccagg actgttgccg gcttccatgg
     2701 tagacctcag cgatgtgctg gcaaaacccg ccgttattct gagcgcccct gccctgagcc
     2761 agtttgtcat tagcaaaccc catcccaaca tgccgcacac cgtcagcatc atccccttta
     2821 acccatcggg tacagacccg gcgtttatta gtacgtggca ggccgcgtca cagaatatgg
     2881 tgtacaacac atccaccgcg cccttaaaac cggccaccgg tagttcacag acggtgtcag
     2941 tcaaggcggt tgctcaaggg gccgtgatta ctgcgacaac ggtgccgcag gcaatgccag
     3001 cgcggggtac cggaggggag ttgcctgtaa tgtcagcgtc cactcctgca agagatcagg
     3061 tcgctgcatg ttttgtcgca gagaacaccg gagattctcc cgacaacccg agctctttcc
     3121 tgacgtcatg tcacccttgc gatccgaaca cggttatagt ggcccagcaa tttcaaccac
     3181 cgcaatgcgt tacgttgttg caggttacct gtgccccctc ttcgacacca ccccccgatt
     3241 caacagtccg ggccccggtg gtgcagttgc caacagtagt ccctctgccg gccagcgcgt
     3301 tcctcccggc gctcgcccaa ccagaagcct cgggcgaaga gcttccgggc ggtcatgacg
     3361 gagaccaagg tgtgccgtgt agagattcaa cggcggcggc tacggcggca gaggcgacaa
     3421 cacccaaacg aaagcagaga agcaaagaga ggagctcaaa gaagcgtaag gctttgaccg
     3481 tgccagaagc cgacaccacg ccatcgacca cgacacctgg tacctctttg ggatcaatta
     3541 ccacccccca ggatgtgcac gccacggatg tcgccacgtc tgagggacca tcggaggcac
     3601 aacccccgct actgtcgtta cccccgccac tggacgtaga tcagagtcta ttcgccctgt
     3661 tagacgaagc gggccctgaa acatgggatg tcgggtcgcc tctctccccc actgacgacg
     3721 cgctgttgtc cagtattctg caaggactgt accagctgga cacgccaccg cctctgcggt
     3781 caccctcccc cgcttccttc ggcccggagt ctccggcgga tataccgtca ccttctggtg
     3841 gagaggaatt ccaccacact ggactagtgg atccgagctc ggtaccaagc ttaagtttaa
     3901 accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     3961 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4021 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4081 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4141 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     4201 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4261 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4321 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4381 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4441 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4501 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4561 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     4621 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4681 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4741 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4801 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4861 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     4921 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     4981 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     5041 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5101 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5161 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5221 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5281 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5341 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5401 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5461 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5521 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     5581 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5641 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5701 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5761 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5821 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5881 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5941 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     6001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6121 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6181 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6241 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6301 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6361 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6421 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6481 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6541 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6601 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6661 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6721 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6781 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6841 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6901 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6961 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7021 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7081 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7141 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7201 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7261 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7321 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7381 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7441 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7501 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7561 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7621 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7681 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7741 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7801 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7861 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7921 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7981 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8041 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8101 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8161 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8221 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8281 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
