LOCUS       sequence-359430-        8336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  antiRTA3kb Human herpesvirus 8 strain JSC-1 clone BAC16, complete 
            genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8336)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0238P
FEATURES             Location/Qualifiers
     source          1..8336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3889..3907)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3890..3907)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3927..3944)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3933..4157
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4203..4631
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4290..4309)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4500..4521
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4640..4660)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4645..4974
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4825..4960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4887..4906
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5041..5835
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5095..5114)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5705..5724
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6009..6130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6046..6065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6100..6119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6179..6195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6179..6195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6192..6214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6203..6219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6227..6233,6234..6251,6252..6257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6272..6293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6410..6427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6581..7169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6661..6680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7340..8131,8132..8200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7963..7982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8201..8305)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctctccac cagaaggtga
      961 cggtatatcc gccggagact ccgggccgaa ggaagcgggg gagggtgacc gcagaggcgg
     1021 tggcgtgtcc agctggtaca gtccttgcag aatactggac aacagcgcgt cgtcagtggg
     1081 ggagagaggc gacccgacat cccatgtttc agggcccgct tcgtctaaca gggcgaatag
     1141 actctgatct acgtccagtg gcgggggtaa cgacagtagc gggggttgtg cctccgatgg
     1201 tccctcagac gtggcgacat ccgtggcgtg cacatcctgg ggggtggtaa ttgatcccaa
     1261 agaggtacca ggtgtcgtgg tcgatggcgt ggtgtcggct tctggcacgg tcaaagcctt
     1321 acgcttcttt gagctcctct ctttgcttct ctgctttcgt ttgggtgttg tcgcctctgc
     1381 cgccgtagcc gccgccgttg aatctctaca cggcacacct tggtctccgt catgaccgcc
     1441 cggaagctct tcgcccgagg cttctggttg ggcgagcgcc gggaggaacg cgctggccgg
     1501 cagagggact actgttggca actgcaccac cggggcccgg actgttgaat cggggggtgg
     1561 tgtcgaagag ggggcacagg taacctgcaa caacgtaacg cattgcggtg gttgaaattg
     1621 ctgggccact ataaccgtgt tcggatcgca agggtgacat gacgtcagga aagagctcgg
     1681 gttgtcggga gaatctccgg tgttctctgc gacaaaacat gcagcgacct gatctcttgc
     1741 aggagtggac gctgacatta caggcaactc ccctccggta ccccgcgctg gcattgcctg
     1801 cggcaccgtt gtcgcagtaa tcacggcccc ttgagcaacc gccttgactg acaccgtctg
     1861 tgaactaccg gtggccggtt ttaagggcgc ggtggatgtg ttgtacacca tattctgtga
     1921 cgcggcctgc cacgtactaa taaacgccgg gtctgtaccc gatgggttaa aggggatgat
     1981 gctgacggtg tgcggcatgt tgggatgggg tttgctaatg acaaactggc tcagggcagg
     2041 ggcgctcaga ataacggcgg gttttgccag cacatcgctg aggtctacca tggaagccgg
     2101 caacagtcct ggctccagga ttggcaaaat taaagattct ggcatgagac cgcttcctgg
     2161 ataggacagt ccagtgacct tgccggtggt aattggccgg cgtttctcag cagaaccggt
     2221 gatgctgtaa aagttttcag gggcgagctg acccttggtc atgatctcca gcggcgccat
     2281 aatcctcgga aggtagaccg gttggaaaac caaaaggcag agggccctgg actgctcaga
     2341 gagcagcccg gcggtatcgt acgtgttgta gagcttcagt agggaagagg ttaatatcat
     2401 aatgggcctc cgattgcaga cgagtcgcct ccaggccccc aggcatttgg ccttcatttc
     2461 agtgtatatt tcgtgtagta gcttacatat ggctggaggc aggctcaccc cgctgcatgc
     2521 ggctgtcaga aatgcctttt ctgtgcagaa taagatcctc tccgtgagga tccgaataat
     2581 gccttgggat gcctctgcgc atggcacgtt gtacccggcc gcgcctttgg tcctcattcg
     2641 agaggcacga aaaaagcaga aaaggttaaa catgttaatg tctgtaaaca gaccacatag
     2701 cgcaccaagc ttggaacatt ctttcataat ccgaatgcac acatcttcca ccactctatt
     2761 taggtcactg ggatcgtaga ttgtttcgta tgcgtcattt ataaggagca cacactggta
     2821 gagttgggcc ttcagttcgt ccgagaggcc gacgaagctt tccacacagg accgccgaag
     2881 cttcttaccc taaggagaga gtggcgtgtc atagttttta caatgacatc gagaaggccc
     2941 cttaaagatc acctgttcaa ccatctgttt aggtatcatt acccgtcctg ggatcagata
     3001 cttcaggagt tagataccct gtccgtcgcc accctcaatc cagactgcca cgtacccgct
     3061 ctaaacgttg agaagaccct ataccttgca aagaccattc agatattggt ccagcaccga
     3121 caaagcgagc cgtacctggt tcccgcggct agggcgaatt tggcttattc cctgcagcag
     3181 ttgtacaaac ttggcaacga caagataagg ggtgttatta acggcatgct gccactagtg
     3241 gacgctggct gcataggttt tgagagagag ctcattaagg gactgcccag agttttgact
     3301 ctccaatacc cccacacggc gcccctggaa tcagaacccc cgaccgcgga ctgcacagag
     3361 tggtgcctct cccattttgt gggagcgtcc ggccgtctcc gctccgaagt tagggatatc
     3421 ctaacaacgc acaacgggac gtgtgctccc tcctttcagt ggatggccag cgtggtaaaa
     3481 aagtttttct tggtagaaac tgtcatttac gaggactttc aggatacaga ttttaacgtg
     3541 cagctgaacc tttgtttttt ttggacagct gtcgttcaga tgtaccagcg gtgcatttac
     3601 gagcagaagc ttgtccacat aatcagcacg tccctaactc tcctgaaaag caccgcccga
     3661 tccttttttg cctggtacga cctgtacaga cccaacctag gttccgcggc tttggtcaag
     3721 tacaccgaac acttaataag agccctgaca cccgactgtt cggacgtaga gcttggcgaa
     3781 ctctgctccc acctacacca ttgtaaacac gcattgttca gtatacaata aaaaggtcga
     3841 tctttacctt gtcagcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca
     3901 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3961 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4021 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4081 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4141 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4201 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4261 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4321 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4381 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4441 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4501 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4561 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4621 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4681 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4741 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4801 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4861 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4921 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4981 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     5041 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5101 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5161 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5221 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5281 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5341 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5401 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5461 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5521 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5581 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5641 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5701 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5761 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5821 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5881 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5941 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6001 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6061 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6121 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6181 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6241 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6301 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6361 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6421 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6481 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6541 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6601 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6661 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6721 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6781 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6841 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6901 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6961 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7021 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7081 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7141 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7201 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7261 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7321 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7381 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7441 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7501 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7561 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7621 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7681 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7741 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7801 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7861 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7921 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7981 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8041 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8101 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8161 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8221 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8281 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
