LOCUS       sequence-359435-        6195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC4A7_STOP
FEATURES             Location/Qualifiers
     source          1..6195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3752..3851)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3868..3887)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3869..3887)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3892..3908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3892..3908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4010..4029)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4021..4830
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4088..4107)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4977..5565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5466..5485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5895..5922)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6014..6100)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6149..6171
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6163..6180
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6164..6180
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga gaggttccgc
      121 ctggagaaga agctgcctgg cccagatgag gaggccgtgg tggacctggg caagaccagc
      181 tccacagtga ataccaagtt tgagaaggag gagctggaga gccacagggc cgtgtacatc
      241 ggagtgcacg tgcccttctc caaggagtct cggagaaggc accggcacag aggccacaag
      301 caccaccacc gccggagaaa ggacaaggag tctgataagg aggacggccg ggagtctcct
      361 agctatgata ccccaagcca gagagtgcag tttatcctgg gcacagagga cgatgacgag
      421 gagcacatcc ctcacgatct gttcaccgag atggacgagc tgtgctaccg ggatggcgag
      481 gagtatgagt ggaaggagac agccagatgg ctgaagtttg aggaggatgt ggaggacgga
      541 ggcgataggt ggagcaagcc atacgtggcc accctgagcc tgcactccct gttcgagctg
      601 aggagctgta tcctgaatgg cacagtgatg ctggatatga gggcatccac cctggacgag
      661 atcgcagata tggtgctgga caacatgatc gcctctggcc agctggacga gagcatcagg
      721 gagaatgtgc gcgaggccct gctgaagagg caccaccacc agaacgagaa gcggtttacc
      781 tccagaatcc ccctggtgag atctttcgcc gacatcggca agaagcactc cgatcctcac
      841 ctgctggaga ggaatggaga gggactgtcc gcctctaggc acagcctgag gacaggactg
      901 agcgcctcca acctgtccct gagaggcgag tctccactga gcctgctgct gggacacctg
      961 ctgccatcta gcagggcagg aaccccagca ggatctaggt gcaccacacc cgtgcctaca
     1021 ccacagaaca gccccccttc ctctccctct atcagccggc tgacctccag aagctcccag
     1081 gagtctcaga ggcaggcacc agagctgctg gtgtcccctg cctctgatga catcccaaca
     1141 gtggtcatcc acccaccaga ggaggacctg gaggccgccc tgaagggcga ggagcagaag
     1201 aacgaggaga atgtggatct gaccccagga atcctggcaa gcccacagtc cgcccctggc
     1261 aacctggaca attccaagtc tggcgagatc aagggcaatg gctctggcgg cagcagggag
     1321 aactccaccg tggatttctc taaggtggac atgaacttca tgcgcaagat ccctacagga
     1381 gcagaggcaa gcaacgtgct ggtgggagag gtggacttcc tggagaggcc catcatcgcc
     1441 tttgtgcgcc tggcacctgc cgtgctgctg acaggactga ccgaggtgcc agtgcctacc
     1501 agattcctgt ttctgctgct gggaccagca ggcaaggcac ctcagtacca cgagatcggc
     1561 aggtccatcg ccacactgat gaccgatgag atctttcacg acgtggccta taaggccaag
     1621 gacagaaatg atctgctgtc cggcatcgat gagttcctgg accaggtgac cgtgctgcct
     1681 ccaggcgagt gggatccttc tatccggatc gagcccccta agtccgtgcc ttctcaggag
     1741 aagagaaaga tcccagtgtt ccacaacgga agcaccccca cactgggaga gacccctaag
     1801 gaggcagcac accacgcagg accagagctg cagaggacag gccgcctgtt tggcggcctg
     1861 atcctggaca tcaagcgcaa ggcccccttc tttctgagcg acttcaagga tgccctgagc
     1921 ctgcagtgcc tggcctccat cctgtttctg tactgcgcct gtatgtcccc agtgatcaca
     1981 ttcggcggac tgctgggaga ggcaaccgag ggaaggatca gcgccatcga gtccctgttt
     2041 ggagcaagcc tgaccggaat cgcatactcc ctgttcgcag gacagccact gacaatcctg
     2101 ggatccaccg gacccgtgct ggtgttcgag aagatcctgt ataagttttg ccgggattac
     2161 cagctgagct atctgtccct gagaacatct atcggcctgt ggaccagctt tctgtgcatc
     2221 gtgctggtgg ccaccgacgc ctctagcctg gtgtgctaca tcacaaggtt caccgaggag
     2281 gcctttgccg ccctgatctg tatcatcttc atctacgagg ccctggagaa gctgttcgac
     2341 ctgggcgaga catatgcctt taatatgcac aacaatctgg ataagctgac aagctattcc
     2401 tgcgtgtgca ccgagccacc caacccaagc aatgagacac tggcccagtg gaagaaggat
     2461 aacatcaccg cccacaatat ctcttggagg aacctgacag tgagcgagtg caagaagctg
     2521 aggggcgtgt ttctgggatc cgcctgtgga caccacggac catacatccc tgacgtgctg
     2581 ttctggtgcg tgatcctgtt ctttaccaca ttctttctgt cctctttcct gaagcagttt
     2641 aagaccaagc ggtacttccc cacaaaggtg agatctacca tcagcgactt cgccgtgttt
     2701 ctgacaatcg tgatcatggt gaccatcgat tatctggtgg gcgtgccatc tcccaagctg
     2761 cacgtgcccg agaagtttga gccaacccac ccagagaggg gatggatcat ctccccactg
     2821 ggcgataatc cctggtggac actgctgatc gccgccatcc ctgccctgct gtgcaccatc
     2881 ctgatcttca tggaccagca gatcacagcc gtgatcatca accggaagga gcacaagctg
     2941 aagaagggcg ccggctatca cctggacctg ctgatggtgg gcgtgatgct gggcgtgtgc
     3001 agcgtgatgg gactgccatg gttcgtggca gcaaccgtgc tgtctatcag ccacgtgaac
     3061 tccctgaagg tggagtccga gtgttctgcc ccaggcgagc agcccaagtt tctgggcatc
     3121 cgggagcaga gagtgaccgg cctgatgatc ttcatcctga tgggcctgag cgtgttcatg
     3181 acatccgtgc tgaagtttat ccctatgcca gtgctgtacg gcgtgtttct gtatatgggc
     3241 gtgagctccc tgaagggcat ccagctgttc gaccgcatca agctgtttgg catgcctgcc
     3301 aagcaccagc cagatctgat ctacctgaga tatgtgcctc tgtggaaggt gcacatcttc
     3361 acagtgatcc agctgacctg cctggtgctg ctgtgggtca tcaaggtgag cgccgcagca
     3421 gtggtgttcc ctatgatggt gctggccctg gtgtttgtga ggaagctgat ggacctgtgc
     3481 ttcaccaagc gcgagctgtc ctggctggat gacctgatgc cagagagcaa gaagaagaag
     3541 gaggatgaca agaagaagaa ggagaaggag gaggccgaga gaatgctgca ggatgacgat
     3601 gacacagtgc acctgccttt tgagggcggc tccctgctgc agatcccagt gaaggccctg
     3661 aagtactctc ctgacaagcc agtgtctgtg aagatcagct tcgaggacga gcccaggaag
     3721 aagtatgtgg atgccgagac cagcctgtaa gacccagctt tcttgtacaa agttggcatt
     3781 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     3841 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     3901 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     3961 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     4021 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     4081 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     4141 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     4201 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     4261 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     4321 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     4381 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     4441 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     4501 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     4561 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     4621 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     4681 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     4741 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     4801 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     4861 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     4921 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5761 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     5821 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     5881 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     5941 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     6001 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     6061 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     6121 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     6181 cttaagctcg ggccc
//
