LOCUS       sequence-359436-        4053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC16A1_STOP
FEATURES             Location/Qualifiers
     source          1..4053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1610..1709)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1726..1745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1727..1745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1750..1766)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1750..1766)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1868..1887)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1879..2688
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1946..1965)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2835..3423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3324..3343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3577..3594
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3753..3780)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3872..3958)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4007..4029
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4021..4038
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4022..4038
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgcc acctgcagtg
      121 ggaggaccag tgggatatac cccaccagac ggaggatggg gatgggcagt ggtcatcggc
      181 gccttcatct ccatcggctt ctcttacgcc tttcccaaga gcatcacagt gttctttaag
      241 gagatcgagg gcatctttca cgccaccaca agcgaggtgt cctggatcag ctccatcatg
      301 ctggccgtga tgtatggcgg cggccccatc tctagcatcc tggtgaataa gtacggctcc
      361 cggatcgtga tgatcgtggg aggatgcctg tccggatgtg gactgatcgc cgcctctttc
      421 tgcaacaccg tgcagcagct gtacgtgtgc atcggcgtga tcggaggact gggactggcc
      481 ttcaacctga atcctgccct gacaatgatc ggcaagtact tttataagcg gagaccactg
      541 gcaaacggac tggcaatggc aggaagcccc gtgtttctgt gcaccctggc ccctctgaat
      601 caggtgttct ttggcatctt cggctggagg ggctcctttc tgatcctggg cggcctgctg
      661 ctgaattgct gcgtggcagg cgccctgatg aggcctatcg gacccaagcc tacaaaggcc
      721 ggcaaggata agtctaaggc cagcctggag aaggccggca agtctggcgt gaagaaggac
      781 ctgcacgatg caaacaccga cctgatcggc cggcacccaa agcaggagaa gagatccgtg
      841 ttccagacaa tcaatcagtt tctggatctg accctgttca cacacagggg ctttctgctg
      901 tatctgtccg gcaacgtgat catgttcttt ggcctgttcg cccctctggt gtttctgtcc
      961 tcttatggca agtctcagca ctacagctcc gagaagagcg ccttcctgct gtccatcctg
     1021 gcctttgtgg acatggtggc aaggccaagc atgggactgg tggccaacac caagccaatc
     1081 aggccccgca tccagtactt ctttgcagca agcgtggtgg caaatggcgt gtgccacatg
     1141 ctggcaccac tgtccaccac atatgtgggc ttctgcgtgt acgccggctt ctttggcttc
     1201 gcctttggct ggctgtctag cgtgctgttc gagaccctga tggatctggt gggaccacag
     1261 cggttttcct ctgccgtggg cctggtgaca atcgtggagt gctgtcctgt gctgctggga
     1321 cctccactgc tgggcagact gaatgacatg tacggcgatt acaagtatac ctactgggcc
     1381 tgtggcgtgg tgctgatcat cagcggcatc tatctgttca tcggcatggg catcaactac
     1441 agactgctgg ccaaggagca gaaggccaat gagcagaaga aggagtctaa ggaggaggag
     1501 acaagcatcg acgtggccgg caagccaaac gaggtgacca aggccgccga gtctcccgat
     1561 cagaaggaca cagatggcgg ccctaaggag gaggagagcc cagtgtaaga cccagctttc
     1621 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1681 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1741 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1801 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1861 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     1921 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1981 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2041 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2101 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2161 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2221 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2281 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2341 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2401 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2461 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2521 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2581 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2641 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2701 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     2761 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     2821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2941 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3001 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3061 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3481 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3541 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3601 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3661 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     3721 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     3781 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     3841 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     3901 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     3961 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4021 tgtaaaacga cggccagtct taagctcggg ccc
//
