LOCUS       sequence-359443-        9320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Orf 63 tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9320)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0339
FEATURES             Location/Qualifiers
     source          1..9320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    914..938
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      977..986
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3767..3832
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     3942..3961
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4133..4157)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4168..4170,4171..4173,4174..4887)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4201..4229)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4824..4844
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4909..4926)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4915..5139
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5185..5613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5272..5291)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5482..5503
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5622..5642)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5627..5956
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5807..5942
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5869..5888
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6023..6817
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6077..6096)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6687..6706
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6993..7114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7030..7049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7084..7103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7163..7179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7163..7179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7176..7198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7187..7203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7211..7217,7218..7235,7236..7241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7256..7277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7394..7411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7565..8153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7645..7664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8324..9115,9116..9184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8947..8966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9185..9289)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttgat caaacaagtt tgtacaaaaa agcaggctct ttaaaggaac caattcagtc
      961 gactggatcc ggtaccgcca ccatggacgg cacagacgct cttgaaaagc ttaccaaagg
     1021 cctgagtggg ggaggcggga gccttcacca gacaaagctg ttgatggaat ttcaactccg
     1081 aggactgccg gtgcctgccc tcttaaacag cagcacaaca gagcagtttt taaatactgt
     1141 tgcccaactg ccgacggacc tatcaaaatt tatacgcgac tatcgcgtgt tcgcactggt
     1201 tcgcgcggcg tattttttag aacccccttc tagcatcgac ccccttgagg cagcgcgcgc
     1261 tcttggacgc ctggttgata tattatcatc acaaccaccg cagaacaccg caccggcgca
     1321 gccacccacc tccgacgaca ccctgaataa ctgtacattg ctcaaactac tagcccacta
     1381 cgcggatcag atagcaggtt tcaaaacccc cgctctccct cccgtgccac ctggaatcat
     1441 cggcctgttc acatgcgtgg aacagatgta ccacgcatgt tttcagaaat actgggcagc
     1501 tgcactaccc ccaatgtgga tactgacata cgaccctccc acttctccgt tacaggactg
     1561 gcttatagtc gcctatggta acaaggaagg actgctactc ccctctggca taccctcgga
     1621 ggaggtgtta gccaaaacat tagtaacaga acaccacgag ttgttcgtat cgcggtcgaa
     1681 ttcgaccgag accgccgtca ccatgcccgt atccaaagaa cgcgccctcg ccatctaccg
     1741 ggtgttcgcc aagggtgagg tggtggcgga aaatactccc attcttgcct tcaccgacgt
     1801 ggaactatcc acactcaaac cccactatct gttcatctat gattttatca tagaggcatt
     1861 atgcaagagc tacacatact catgcacgca ggcccgcctg gaatcctttt tgagccgagg
     1921 tatagacttc atgactgacc taggtcagta cctagatacc gctactagcg gcaagcagca
     1981 gctgacgcac agccaaataa aggaaatcaa atacaggctg ctaagctgcg gtctctcggc
     2041 ttccgcgtgt gatgttttca gaactgtgat catgaccctc ccatatcgac cgacccccaa
     2101 cctcgctaac ctgtccacgt ttatggggat ggttcaccaa ctgaccatgt tcggacacta
     2161 tttctaccgg tgcctgggca gctacagtcc caccggcttg gccttcacag aattgcaaaa
     2221 gatactgaca cgcgccagcg cggagcaaac ggaacgtaac ccgtggagac atccgggtat
     2281 ctcggacatt ccactgcgtt ggaaaatatc gcgtgctcta gcattcttcg tcactccggc
     2341 ccccataaac actttgcagc gcgtgtacgc cgcgctgccc tcgcaactca tgcgggccat
     2401 cttcgagatc tcggtcaaga ccacatgggg aggcgccgta ccggcaaacc tggcgcgcga
     2461 cattgacaca ggaccgaaca cacaacatat ctcctccaca ccaccgccca ccctcaagga
     2521 tgttgagaca tactgtcaag gtctgcgggt gggagacacg gagtacgatg aggacattgt
     2581 gagaagcccg ctctttgcag acgcgtttac caagagtcac ttgttgccta tactgcgcga
     2641 ggttctggaa aaccgcctgc agaaaaacag agctctgttt cagataagat ggctgataat
     2701 atttgctgcc gaggcggcaa ccgggctcat ccctgccagg cgcccgctag ccagagccta
     2761 cttccacatc atggacattc tggaggagag acattcccaa gacgccctat acaacctttt
     2821 ggactgtatc caggagctct tcacccacat caggcaggct gttccagacg cacagtgtcc
     2881 gcacgccttt ctacagtccc tgttcgtctt tcaattccgc cctttcgtac tcaaacacca
     2941 gcagggtgta accttgtttc tagatggctt gcagacatcc ctccccccgg tgataagtct
     3001 ggccaacctt ggagacaagc tgtgtcgtct cgagttcgag tacgacagcg agggcgactt
     3061 cgtgcgcgtg ccagttgcac cgccagaaca accaccgcac gtacatctgt cgcatttcaa
     3121 gaagacaata cagaccatcg aacaggccac cagggaggcc accgtagcca tgacaacaat
     3181 cgcaaagcca atataccccg cctacatccg gttactgcag cggctagaat atcttaacag
     3241 actcaaccac cacattctca ggattccctt cccacaggac gccctttctg aactccagga
     3301 aacctacctg gcggcgtttg cacggttgac aaaattggca gcggacgcag caaacacttg
     3361 tagctactcc ctcaccaagt actttggagt tttattccaa caccagctgg tccccacggc
     3421 catcgttaaa aaactgctac atttcgacga ggctaaagat accacagaag cctttttaca
     3481 gagcctggca caacccgtag tgcagggaca acggcagggg gcggctggcg ggtcgggtgt
     3541 cctgacgcag aaagaacttg agctcttgaa caaaataaac ccacagttta cagacgctca
     3601 ggctaacatt cctccatcta ttaaacgttc atattcaaat aaatatgacg tccctgaggt
     3661 ctcagtcgac tgggaaacgt actcccggtc tgccttcgag gcaccggacg acgaactccg
     3721 ttttgtccca ctgacgctgg caggcctccg gaaactgttt gtcgaagact acaaagacca
     3781 tgacggtgat tataaagatc atgatatcga ttacaaggat gacgatgaca agtagtaacc
     3841 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtcccccg
     3901 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     3961 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     4021 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     4081 acacagccag tctgcaggtc gacgaattcg cggccgcact cgagatatct agacccagct
     4141 ttcttgtaca aagtggttcg atctagaatg gctagcaaag gagaagaact tttcactgga
     4201 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     4261 ggagagggtg aaggtgatgc tacatacgga aagcttaccc ttaaatttat ttgcactact
     4321 ggaaaactac ctgttccatg gccaacactt gtcactactt tctcttatgg tgttcaatgc
     4381 ttttcccgtt atccggatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa
     4441 ggttatgtac aggaacgcac tatatctttc aaagatgacg ggaactacaa gacgcgtgct
     4501 gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaagg tattgatttt
     4561 aaagaagatg gaaacattct cggacacaaa ctcgagtaca actataactc acacaatgta
     4621 tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat tcgccacaac
     4681 attgaagatg gatccgttca actagcagac cattatcaac aaaatactcc aattggcgat
     4741 ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgccct ttcgaaagat
     4801 cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca
     4861 catggcatgg atgagctcta caaataatga attaaacccg ctgatcagcc tcgactgtgc
     4921 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4981 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5041 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5101 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     5161 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5221 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5281 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5341 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5401 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5461 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5521 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5581 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5641 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5701 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5761 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5821 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5881 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5941 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     6001 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     6061 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     6121 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     6181 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     6241 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     6301 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     6361 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6421 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6481 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6541 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6601 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6661 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6721 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6781 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa
     6841 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6901 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6961 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     7021 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7081 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     7141 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7201 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7261 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7321 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9301 gaaaagtgcc acctgacgtc
//
