LOCUS       sequence-359448-        8337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8337)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0273P
FEATURES             Location/Qualifiers
     source          1..8337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3890..3908)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3891..3908)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3928..3945)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3934..4158
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4204..4632
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4291..4310)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4501..4522
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4641..4661)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4646..4975
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4826..4961
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4888..4907
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5042..5836
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5096..5115)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5706..5725
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6010..6131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6047..6066)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6101..6120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6180..6196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6180..6196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6193..6215)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6204..6220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6228..6234,6235..6252,6253..6258))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6273..6294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6411..6428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6582..7170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6662..6681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7341..8132,8133..8201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7964..7983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8202..8306)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcctctcca ccagaaggtg
      961 acggtatatc cgccggagac tccgggccga aggaagcggg ggagggtgac cgcagaggcg
     1021 gtggcgtgtc cagctggtac agtccttgca gaatactgga caacagcgcg tcgtcagtgg
     1081 gggagagagg cgacccgaca tcccatgttt cagggcccgc ttcgtctaac agggcgaata
     1141 gactctgatc tacgtccagt ggcgggggta acgacagtag cgggggttgt gcctccgatg
     1201 gtccctcaga cgtggcgaca tccgtggcgt gcacatcctg gggggtggta attgatccca
     1261 aagaggtacc aggtgtcgtg gtcgatggcg tggtgtcggc ttctggcacg gtcaaagcct
     1321 tacgcttctt tgagctcctc tctttgcttc tctgctttcg tttgggtgtt gtcgcctctg
     1381 ccgccgtagc cgccgccgtt gaatctctac acggcacacc ttggtctccg tcatgaccgc
     1441 ccggaagctc ttcgcccgag gcttctggtt gggcgagcgc cgggaggaac gcgctggccg
     1501 gcagagggac tactgttggc aactgcacca ccggggcccg gactgttgaa tcggggggtg
     1561 gtgtcgaaga gggggcacag gtaacctgca acaacgtaac gcattgcggt ggttgaaatt
     1621 gctgggccac tataaccgtg ttcggatcgc aagggtgaca tgacgtcagg aaagagctcg
     1681 ggttgtcggg agaatctccg gtgttctctg cgacaaaaca tgcagcgacc tgatctcttg
     1741 caggagtgga cgctgacatt acaggcaact cccctccggt accccgcgct ggcattgcct
     1801 gcggcaccgt tgtcgcagta atcacggccc cttgagcaac cgccttgact gacaccgtct
     1861 gtgaactacc ggtggccggt tttaagggcg cggtggatgt gttgtacacc atattctgtg
     1921 acgcggcctg ccacgtacta ataaacgccg ggtctgtacc cgatgggtta aaggggatga
     1981 tgctgacggt gtgcggcatg ttgggatggg gtttgctaat gacaaactgg ctcagggcag
     2041 gggcgctcag aataacggcg ggttttgcca gcacatcgct gaggtctacc atggaagccg
     2101 gcaacagtcc tggctccagg attggcaaaa ttaaagattc tggcatgaga ccgcttcctg
     2161 gataggacag tccagtgacc ttgccggtgg taattggccg gcgtttctca gcagaaccgg
     2221 tgatgctgta aaagttttca ggggcgagct gacccttggt catgatctcc agcggcgcca
     2281 taatcctcgg aaggtagacc ggttggaaaa ccaaaaggca gagggccctg gactgctcag
     2341 agagcagccc ggcggtatcg tacgtgttgt agagcttcag tagggaagag gttaatatca
     2401 taatgggcct ccgattgcag acgagtcgcc tccaggcccc caggcatttg gccttcattt
     2461 cagtgtatat ttcgtgtagt agcttacata tggctggagg caggctcacc ccgctgcatg
     2521 cggctgtcag aaatgccttt tctgtgcaga ataagatcct ctccgtgagg atccgaataa
     2581 tgccttggga tgcctctgcg catggcacgt tgtacccggc cgcgcctttg gtcctcattc
     2641 gagaggcacg aaaaaagcag aaaaggttaa acatgttaat gtctgtaaac agaccacata
     2701 gcgcaccaag cttggaacat tctttcataa tccgaatgca cacatcttcc accactctat
     2761 ttaggtcact gggatcgtag attgtttcgt atgcgtcatt tataaggagc acacactggt
     2821 agagttgggc cttcagttcg tccgagaggc cgacgaagct ttccacacag gaccgccgaa
     2881 gcttcttacc ctaaggagag agtggcgtgt catagttttt acaatgacat cgagaaggcc
     2941 ccttaaagat cacctgttca accatctgtt taggtatcat tacccgtcct gggatcagat
     3001 acttcaggag ttagataccc tgtccgtcgc caccctcaat ccagactgcc acgtacccgc
     3061 tctaaacgtt gagaagaccc tataccttgc aaagaccatt cagatattgg tccagcaccg
     3121 acaaagcgag ccgtacctgg ttcccgcggc tagggcgaat ttggcttatt ccctgcagca
     3181 gttgtacaaa cttggcaacg acaagataag gggtgttatt aacggcatgc tgccactagt
     3241 ggacgctggc tgcataggtt ttgagagaga gctcattaag ggactgccca gagttttgac
     3301 tctccaatac ccccacacgg cgcccctgga atcagaaccc ccgaccgcgg actgcacaga
     3361 gtggtgcctc tcccattttg tgggagcgtc cggccgtctc cgctccgaag ttagggatat
     3421 cctaacaacg cacaacggga cgtgtgctcc ctcctttcag tggatggcca gcgtggtaaa
     3481 aaagtttttc ttggtagaaa ctgtcattta cgaggacttt caggatacag attttaacgt
     3541 gcagctgaac ctttgttttt tttggacagc tgtcgttcag atgtaccagc ggtgcattta
     3601 cgagcagaag cttgtccaca taatcagcac gtccctaact ctcctgaaaa gcaccgcccg
     3661 atcctttttt gcctggtacg acctgtacag acccaaccta ggttccgcgg ctttggtcaa
     3721 gtacaccgaa cacttaataa gagccctgac acccgactgt tcggacgtag agcttggcga
     3781 actctgctcc cacctacacc attgtaaaca cgcattgttc agtatacaat aaaaaggtcg
     3841 atctttacct tgtcagcggc cgctcgagca tgcatctaga gggccctatt ctatagtgtc
     3901 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     3961 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4021 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4081 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4141 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     4201 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4261 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4321 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4381 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4441 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4501 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4561 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4621 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4681 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4741 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4801 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4861 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4921 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4981 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     5041 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5101 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5161 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5221 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5281 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5341 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5401 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5461 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5521 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5581 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5641 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5701 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5761 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5821 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5881 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5941 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6001 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6061 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6121 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     6181 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6241 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6301 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6361 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6421 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6481 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6541 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6601 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6661 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6721 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6781 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6841 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6901 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6961 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7021 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7081 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7141 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7201 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7261 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7321 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7381 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7441 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7501 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7561 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7621 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7681 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7741 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7801 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7861 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7921 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7981 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8041 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8101 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8161 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8221 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8281 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
