LOCUS       sequence-359474-        2591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_251
FEATURES             Location/Qualifiers
     source          1..2591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(81..185)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      288..876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     777..796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1811..2591,1..11,12..80))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2434..2453
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
       61 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
      121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
      181 ccgcgcacat ttccccgaaa agtgccacct gtcatgacca aaatccctta acgtgagttt
      241 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
      301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
      361 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
      421 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
      481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      541 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      601 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      661 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      721 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      781 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      841 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      901 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      961 tctgtggata accgtagtcg gttctagaga ctagtgggtc tcatatggtc agtaaaggcg
     1021 aagcagttat caaagagttc atgcgcttca aagttcatat ggaagggtcg atgaacgggc
     1081 acgaatttga aattgaaggc gaaggcgaag gccgcccata tgaagggacc caaaccgcaa
     1141 agcttaaggt tactaaaggc ggtccattac ccttttcgtg ggacatttta agcccacagt
     1201 ttatgtacgg gagtcgcgct ttcatcaagc accctgcgga catcccagat tactacaaac
     1261 agtctttccc cgaggggttc aagtgggagc gcgtgatgaa cttcgaggat ggcggagccg
     1321 tgacggtcac ccaagatacc tctttggagg acggtacgtt gatctacaaa gtgaaattgc
     1381 gtggcacgaa ttttccacct gatgggcctg tcatgcagaa aaagacaatg ggatgggaag
     1441 cttccacgga gcgcctttac ccagaggacg gtgttcttaa aggggatatc aaaatggcgc
     1501 tgcgtcttaa agatggaggc cgctacctgg cggacttcaa gactacttac aaggccaaaa
     1561 aaccagtgca gatgccgggt gcgtacaatg tagatcgtaa attagatatt acaagtcaca
     1621 atgaagatta cacggtcgta gagcagtatg agcgcagtga ggggcgtcac tctacgggcg
     1681 gtatggacga gttatacaag taattcttga gaccaagatt atcaaaaagg atcttcacct
     1741 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1801 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1861 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1921 catctggccc cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat
     1981 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2041 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2101 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2161 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2221 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2281 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2341 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2401 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2461 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2521 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2581 ctttcaccag c
//
