LOCUS       sequence-359481-        8321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reverse complement antiRTA3kb pOME0238 Human herpesvirus 8 strain 
            JSC-1 clone BAC16, complete genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8321)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0239M
FEATURES             Location/Qualifiers
     source          1..8321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3912..3929)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3918..4142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4188..4616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4275..4294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4485..4506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4625..4645)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4630..4959
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4810..4945
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4872..4891
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5026..5820
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5080..5099)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5690..5709
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5994..6115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6031..6050)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6085..6104
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6164..6180)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6164..6180)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6177..6199)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6188..6204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6212..6218,6219..6236,6237..6242))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6257..6278
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6395..6412)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6566..7154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6646..6665)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7325..8116,8117..8185))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7948..7967
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8186..8290)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgctgacaag gtaaagatcg acctttttat
      961 tgtatactga acaatgcgtg tttacaatgg tgtaggtggg agcagagttc gccaagctct
     1021 acgtccgaac agtcgggtgt cagggctctt attaagtgtt cggtgtactt gaccaaagcc
     1081 gcggaaccta ggttgggtct gtacaggtcg taccaggcaa aaaaggatcg ggcggtgctt
     1141 ttcaggagag ttagggacgt gctgattatg tggacaagct tctgctcgta aatgcaccgc
     1201 tggtacatct gaacgacagc tgtccaaaaa aaacaaaggt tcagctgcac gttaaaatct
     1261 gtatcctgaa agtcctcgta aatgacagtt tctaccaaga aaaacttttt taccacgctg
     1321 gccatccact gaaaggaggg agcacacgtc ccgttgtgcg ttgttaggat atccctaact
     1381 tcggagcgga gacggccgga cgctcccaca aaatgggaga ggcaccactc tgtgcagtcc
     1441 gcggtcgggg gttctgattc caggggcgcc gtgtgggggt attggagagt caaaactctg
     1501 ggcagtccct taatgagctc tctctcaaaa cctatgcagc cagcgtccac tagtggcagc
     1561 atgccgttaa taacacccct tatcttgtcg ttgccaagtt tgtacaactg ctgcagggaa
     1621 taagccaaat tcgccctagc cgcgggaacc aggtacggct cgctttgtcg gtgctggacc
     1681 aatatctgaa tggtctttgc aaggtatagg gtcttctcaa cgtttagagc gggtacgtgg
     1741 cagtctggat tgagggtggc gacggacagg gtatctaact cctgaagtat ctgatcccag
     1801 gacgggtaat gatacctaaa cagatggttg aacaggtgat ctttaagggg ccttctcgat
     1861 gtcattgtaa aaactatgac acgccactct ctccttaggg taagaagctt cggcggtcct
     1921 gtgtggaaag cttcgtcggc ctctcggacg aactgaaggc ccaactctac cagtgtgtgc
     1981 tccttataaa tgacgcatac gaaacaatct acgatcccag tgacctaaat agagtggtgg
     2041 aagatgtgtg cattcggatt atgaaagaat gttccaagct tggtgcgcta tgtggtctgt
     2101 ttacagacat taacatgttt aaccttttct gcttttttcg tgcctctcga atgaggacca
     2161 aaggcgcggc cgggtacaac gtgccatgcg cagaggcatc ccaaggcatt attcggatcc
     2221 tcacggagag gatcttattc tgcacagaaa aggcatttct gacagccgca tgcagcgggg
     2281 tgagcctgcc tccagccata tgtaagctac tacacgaaat atacactgaa atgaaggcca
     2341 aatgcctggg ggcctggagg cgactcgtct gcaatcggag gcccattatg atattaacct
     2401 cttccctact gaagctctac aacacgtacg ataccgccgg gctgctctct gagcagtcca
     2461 gggccctctg ccttttggtt ttccaaccgg tctaccttcc gaggattatg gcgccgctgg
     2521 agatcatgac caagggtcag ctcgcccctg aaaactttta cagcatcacc ggttctgctg
     2581 agaaacgccg gccaattacc accggcaagg tcactggact gtcctatcca ggaagcggtc
     2641 tcatgccaga atctttaatt ttgccaatcc tggagccagg actgttgccg gcttccatgg
     2701 tagacctcag cgatgtgctg gcaaaacccg ccgttattct gagcgcccct gccctgagcc
     2761 agtttgtcat tagcaaaccc catcccaaca tgccgcacac cgtcagcatc atccccttta
     2821 acccatcggg tacagacccg gcgtttatta gtacgtggca ggccgcgtca cagaatatgg
     2881 tgtacaacac atccaccgcg cccttaaaac cggccaccgg tagttcacag acggtgtcag
     2941 tcaaggcggt tgctcaaggg gccgtgatta ctgcgacaac ggtgccgcag gcaatgccag
     3001 cgcggggtac cggaggggag ttgcctgtaa tgtcagcgtc cactcctgca agagatcagg
     3061 tcgctgcatg ttttgtcgca gagaacaccg gagattctcc cgacaacccg agctctttcc
     3121 tgacgtcatg tcacccttgc gatccgaaca cggttatagt ggcccagcaa tttcaaccac
     3181 cgcaatgcgt tacgttgttg caggttacct gtgccccctc ttcgacacca ccccccgatt
     3241 caacagtccg ggccccggtg gtgcagttgc caacagtagt ccctctgccg gccagcgcgt
     3301 tcctcccggc gctcgcccaa ccagaagcct cgggcgaaga gcttccgggc ggtcatgacg
     3361 gagaccaagg tgtgccgtgt agagattcaa cggcggcggc tacggcggca gaggcgacaa
     3421 cacccaaacg aaagcagaga agcaaagaga ggagctcaaa gaagcgtaag gctttgaccg
     3481 tgccagaagc cgacaccacg ccatcgacca cgacacctgg tacctctttg ggatcaatta
     3541 ccacccccca ggatgtgcac gccacggatg tcgccacgtc tgagggacca tcggaggcac
     3601 aacccccgct actgtcgtta cccccgccac tggacgtaga tcagagtcta ttcgccctgt
     3661 tagacgaagc gggccctgaa acatgggatg tcgggtcgcc tctctccccc actgacgacg
     3721 cgctgttgtc cagtattctg caaggactgt accagctgga cacgccaccg cctctgcggt
     3781 caccctcccc cgcttccttc ggcccggagt ctccggcgga tataccgtca ccttctggtg
     3841 gagagaattc caccacactg gactagtgga tccgagctcg gtaccaagct taagtttaaa
     3901 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3961 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4021 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4081 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4141 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4261 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4381 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4501 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4561 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4621 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4681 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4741 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4801 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4861 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4921 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4981 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     5041 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5101 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5161 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     5221 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5281 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5341 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5401 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5461 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5521 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5581 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5641 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5701 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5761 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5821 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5881 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5941 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6001 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6061 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6121 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6181 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6241 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6301 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6361 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6421 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6481 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6541 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6601 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6661 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6721 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6781 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6841 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6901 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6961 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7021 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7081 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7141 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7201 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7261 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7321 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7381 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7441 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7501 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7561 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7621 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7681 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7741 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7801 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7861 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7921 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7981 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8041 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8101 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8161 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8221 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8281 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
